US4339537A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of culturing biological substances
Assignee: Olympus Corp
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Filing: 1979-11-06
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Granted: 1982-07-13
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Expires: 1999-11-06
Abstract
Abstract Biological substances such as tissues, cells, vaccines and viruses are cultured by the steps of: introducing a culture solution having the substances suspended therein into a closed culture vessel having a plurality of disc-like implantation plates arranged horizontally with a certain space between successive plates so that a predetermined number of the implantation plates is immersed in the culture solution; culturing the substances on the surface of the implantation plates; removing the substances from the implantation plates; suspending the removed cultured substances in a new culture solution; and successively repeating the steps of culturing, removing the cultured substances and suspending in a new culture solution while each time increasing the amount of newly supplied culture solution to progressively increase the number of implantation plates immersed in the culture solution. This process enables production of a large amount of biological substance from a small amount of seed substance by mass production in a simple operation without any contamination.
Inventors
- 👤 Shinroku Sogi
- 👤 Masao Izawa
- 👤 Shinichiro Hattori
- 👤 Ikuo Tawara
- 👤 Daizo Shinohara
- 👤 Sachiko Tachikawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 15:35
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/04
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
239 words
A method of culturing biological substances capable of propagation in a culture solution, said method comprising culturing the biological substances in a substantially closed culture space formed by a culture vessel containing a plurality of implantation plates which are arranged substantially horizontally and spaced from each other in a vertical direction by the steps of: (a) introducing a given small amount of biological substances together with a given amount of culture solution into the culture vessel to such a level that a predetermined number of the implantation plates is immersed in the culture solution having the biological substances suspended therein; (b) propagating the biological substances on the surface of the immersed implantation plates, while an atmosphere inside the culture vessel is controlled in a desired manner; (c) introducing into the culture vessel an agent which makes it easy to peel the propagated biological substances from the surface of the immersed implantation plates, peeling the biological substances from the plates and then discharging the agent; (d) introducing a culture solution into the culture vessel to suspend uniformly the peeled biological substances therein; (e) repeating said steps (b), (c) and (d) in succession, while the propagated biological substances are kept in the culture vessel and the number of implantation plates to be immersed in the culture solution having the biological substances suspended therein is progressively increased by increasing the amount of culture solution introduced into the culture vessel in step (d).
Claim 2
Independent Claim
239 words
A method of culturing biological substances capable of propagation in a culture solution, said method comprising culturing the biological substances in a substantially closed culture space formed by a culture vessel containing a plurality of implantation plates which are arranged substantially horizontally and spaced from each other in a vertical direction by the steps of: (a) introducing a given small amount of biological substances together with a given amount of culture solution into the culture vessel to such a level that a predetermined number of the implantation plates is immersed in the culture solution having the biological substances suspended therein; (b) propagating the biological substances on the surface of the immersed implantation plates, while an atmosphere inside the culture vessel is controlled in a desired manner; (c) introducing into the culture vessel an agent which makes it easy to peel the propagated biological substances from the surface of the immersed implantation plates, peeling the biological substances from the plates and then discharging the agent; (d) introducing a culture solution into the culture vessel to suspend uniformly the peeled biological substances therein; (e) repeating said steps (b), (c) and (d) in succession, while the propagated biological substances are kept in the culture vessel and the number of implantation plates to be immersed in the culture solution having the biological substances suspended therein is progressively increased by increasing the amount of culture solution introduced into the culture vessel in step (d).
Claim 3
Dependent Claim
44 words
The method according to claim 1, further comprising between the steps (b) and (c), a step of discharging the culture solution when propagation is complete; and a step of washing the propagated substances on the surface of the implantation plates with a buffer solution.
Claim 4
Dependent Claim
25 words
The method according to claim 1, wherein the agent for making it easy to peel the propagated substances from the implantation plates is an enzyme.
Claim 5
Dependent Claim
36 words
The method according to claim 1, further comprising between the steps (c) and (d), a step for moving mechanically the implantation plates so as to promote the peeling of the propagated substances from the implantation plates.
Claim 6
Dependent Claim
30 words
The method according to claim 1, further comprising between the steps (b) and (c), a step for detecting the propagation condition of the biological substances grown on the implantation plates.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-07-12 | 1986-01-11 | 1986-07-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-07-12 | 1990-01-11 | 1990-07-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-07-12 | 1994-01-11 | 1994-07-13 | $7700.00 | Window Closed / Lapsed |