Immobilization of starch degrading enzymes

Claim 2

In a process for preparing immobilized starch-degrading enzyme consisting essentially of immobilizing by physical adsorption a starch-degrading enzyme onto a water-insoluble carrier, thereby insolubilizing said enzyme, the improvement whereby the activity of the enzyme after physical adsorption is enhanced, comprising, prior to the physical adsorption step, modifying or cross-linking, in solution, the starch-degrading enzyme with a mono- or poly-functional reagent at a pH in the range of 3 to 10 and temperatures in the range of 0° to 80° C., in a manner that said enzyme is not substantially insolubilized.

Claim 3

A process as set forth in claim 1, wherein said starch-degrading enzyme is α-amylase, β-amylase, glucoamylase, isoamylase, or cyclodextrin glucanotransferase.

Claim 4

A process as set forth in claim 1, wherein said modifying or cross-linking step is carried out with said starch-degrading enzyme at a concentration of 0.01 to 5 w/v%, and said mono- or poly-functional reagent at a concentration of 0.001 to 5 w/v%, for 0.1 to 50 hours.

Claim 5

A process as set forth in claim 1, wherein said reagent is selected from the group consisting of diazo compounds including bis-azo-benzidine; acid anhydrides including succinic anhydride and maleic anhydride; carboxylic acids; isocyanate compounds including hexamethylene diisocyanate and toluene 2,4-diisocyanate; isothiocyanate compounds; thioamides; imide compounds including N-acetoxy-succinimide and N,N'-ethylene-bis-maleimide; carbodiimides including 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide metho-p-toluene sulfonate; imidocarbonates; aldehyde compounds including acetaldehyde, propionaldehyde, butyraldehyde, octyl aldehyde, lauraldehyde oleyl aldehyde, benzaldehyde, p-nitrobenzaldehyde, naphthaldehyde, glyoxal, glutaraldehyde, succindiadehyde, dialdehyde starch, and dialdehyde pullulan; acetal compounds; halides including cyanuric chloride; and halosulfonyl compounds.

Claim 6

A process as set forth in claim 1, wherein said water-insoluble carrier is activated carbon, porous glass, Japanese acid clay, bleaching clay, kaolinite, alumina, silica gel, bentonite, ceramics, hydroxylapatite, calcium phosphate gel, starch, amylose, pullulan, dextran, cellulose, porous co-polymer resin, or any of their derivatives.

Claim 7

A process as set forth in claim 1, wherein said water-insoluble carrier is a porous co-polymer resin synthesized by co-polymerizing styrene monomer and one or more members of the group consisting of methyl styrene, nitrostyrene, styryl halide, acrylonitrile, vinyl acetate, vinyl chloride, divinylbenzene, butadiene, and isopurene monomers.

Claim 8

A process in accordance with claim 1, wherein said reagent is glutaraldehyde.

Claim 9

A process in accordance with claim 1, wherein said water-insoluble carrier is porous beads of styrene-divinyl benzene copolymer.

Claim 10

In a process for the production of a reaction product from amylaceous material, comprising subjecting the amylaceous material to the action of a soluble or immobilized starch-degrading enzyme, the improvement wherein the starch-degrading enzyme is an immobilized starch-degrading enzyme obtained by a process in accordance with claim 1.

Claim 11

A process as set forth in claim 9, wherein said reaction product prepared with said immobilized-starch-degrading enzyme is corn syrup, glucose, maltose, cyclodextrin, glycosylsucrose, glycosyllactose, glycosylriboflavin, glycosylesculin, glycosylrutin or glycosylstevioside.