Patent US4336331A successfully refreshed from Google Patents.
US4336331A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for assaying the activity of γ-glutamyl transpeptidase in serum
Assignee: Nitto Boseki Co Ltd
•
Filing: 1981-03-20
•
Granted: 1982-06-22
•
Expires: 2001-03-20
Abstract
Abstract Novel L- gamma -glutamyl-3-carboxy-4-hydroxyanilide of the formula, <IMAGE> an acid addition salt thereof, or an alkali metal or amine salt thereof, which is useful for determining the activity of gamma -glutamyl transpeptidase.
Inventors
- 👤 Takeshi Nagasawa
- 👤 Katsumasa Kuroiwa
- 👤 Katsuyuki Takabayashi
- 👤 Norimasa Takizawa
- 👤 Kazuyo Hagihara
- 👤 Tadami Akatsuka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 13:09
CPC Classifications
C; C07; C07C; C07C237/00; C07C237/02; C07C237/04; C12; C12Q; C12Q1/00; C12Q1/48; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/91; G01N2333/91045; G01N2333/91074; G01N2333/9108; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
70 words
A method for assaying the activity of gamma-glutamyl transpeptidase in serum which comprises allowing the compound L-gamma-glutamyl-3-carboxy-4-hydroxyanilide represented by the formula ##STR3## an acid addition salt thereof, or an alkali metal or amine salt thereof, to react with gamma-glutamyl transpeptidase in serum in a buffer solution, allowing 3-carboxy-4-hydroxyaniline formed by the reaction to undergo oxidative condensation with a coupler and then determining colorimetrically a colored substance formed by the condensation.
Claim 2
Independent Claim
70 words
A method for assaying the activity of gamma-glutamyl transpeptidase in serum which comprises allowing the compound L-gamma-glutamyl-3-carboxy-4-hydroxyanilide represented by the formula ##STR3## an acid addition salt thereof, or an alkali metal or amine salt thereof, to react with gamma-glutamyl transpeptidase in serum in a buffer solution, allowing 3-carboxy-4-hydroxyaniline formed by the reaction to undergo oxidative condensation with a coupler and then determining colorimetrically a colored substance formed by the condensation.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-21 | 1985-12-21 | 1986-06-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-21 | 1989-12-21 | 1990-06-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-21 | 1993-12-21 | 1994-06-22 | $7700.00 | Window Closed / Lapsed |