US4335226A
Expired / Lapsed
US
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Adsorbent for the affinity-specific separation of macromolecular materials
Assignee: Roche Diagnostics GmbH
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Filing: 1978-02-24
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Granted: 1982-06-15
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Expires: 1998-02-24
Abstract
Abstract Adsorbents are provided for the affinity-specific separation of macromolecular materials, particularly biopolymers, the adsorbents comprising a polymeric carrier on to which an affine residue for the macromolecular material is covalently bound, either directly or via polymeric spacer. Preferred affine residue is comprised of a specific complex formers which are base-specific and/or structure specific for the macromolecular material. The invention also provides novel complex formers comprising a certain dyestuff residues and processes for their preparation.
Inventors
- 👤 Werner Muller
- 👤 Hans Bunemann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 08:33
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3255; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3253; C; C07; C07D; C07D219/00; C07D219/04; C07D219/08; C07D219/10; C07D219/12; C07D221/00; C07D221/02; C07D221/04; C07D221/06; C07D221/10; C07D221/12; C08; C08F; C08F265/00; C08F265/10; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1006; C12N15/101
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
34 words
Adsorbent for the affinity-specific separation of nucleic acids, comprising a polymeric carrier material onto which an affine residue group, consisting of a base and/or structure specific complex former for nucleic acids, is covalently bound.
Claim 2
Dependent Claim
34 words
Adsorbent for the affinity-specific separation of nucleic acids, comprising a polymeric carrier material onto which an affine residue group, consisting of a base and/or structure specific complex former for nucleic acids, is covalently bound.
Claim 3
Dependent Claim
20 words
Adsorbent as claimed in claim 1 wherein said affine residue group is directly covalently bound to said polymeric carrier material.
Claim 4
Dependent Claim
23 words
Adsorbent as claimed in claim 1 wherein said affine residue group is covalently bound via a polymeric spacer to said polymeric carrier material.
Claim 5
Dependent Claim
48 words
Adsorbent as claimed in claim 3 wherein said polymeric carrier material is a small-pored, low compressability polymeric carrier material onto which is grafted said affine residue group via, as the polymeric spacer, a copolymer of at least two copolymerizable monomers, one of which carriers said affine residue group.
Claim 6
Dependent Claim
20 words
Adsorbent as claimed in claim 1 wherein said polymeric carrier carries, as the affine residue, a base specific complex former.
Claim 7
Dependent Claim
91 words
Adsorbent as claimed in claim 1 wherein said complex former is a residue of the dyestuff of the formula ##STR7## wherein X is CH or nitrogen; Y is an oxygen or sulfur atom or an NH group, or a group of the formula ##STR8## wherein each R 1 is individually selected from hydrogen and methyl R 2 is hydrogen or methyl; R 3 is hydrogen or methyl; R 4 is hydrogen or methyl; and A.sup.⊖ is an anion, or the residue of an intercalating dyestuff containing a planar polycyclic ring system.
Claim 8
Dependent Claim
55 words
Adsorbent as claimed in claim 1 wherein said complex former is a residue of a dyestuff with a planar polycyclic ring system, and the planar polycyclic ring system of the intercalating dyestuff is a bi-, tri-, or tetracyclic condensed ring system, which optionally contains a hetero atom selected from a nitrogen, oxygen or sulfur atom.
Claim 9
Dependent Claim
46 words
Adsorbent as claimed in claim 1 wherein the polymeric carrier material carries, as base- and/or structure specific complex former, the residue group of malachite green, crystal violet, methyl green, auramine, the dyestuff Hoechst 33258, di-tert.-butyl-proflavine, di-tert.-butylacriflavine, diamidinophenylindole (DAPI), 1-methylphenyl neutral red, ethidium bromide, acridine or 9-(acryloylaminoethylamino)-2-methoxy-6-chloroacridine.
Claim 10
Dependent Claim
20 words
Adsorbent as claimed in claim 1 wherein the base- and/or structure-specific complex former is a radical of the formula ##STR9##
Claim 11
Dependent Claim
20 words
Adsorbent as claimed in claim 1 wherein the base- and/or structure-specific complex former is a radical of the formula ##STR10##
Claim 12
Dependent Claim
55 words
Adsorbent as claimed in claim 1 wherein the polymeric carrier material is a polymer or copolymer of monomers which, in addition to the functional group necessary for the formation of the polymer or copolymer, contain at least one further functional group via which can be covalently bound the polymeric spacer with the affine residue group.
Claim 13
Dependent Claim
66 words
Adsorbent as claimed in claim 1 wherein the polymeric carrier material is a poly-bisacrylamide on to which is grafted the affine residue group in the presence of an excess of a further copolymerizable monomer by copolymerization, or the carrier material is a cross-linked, hydrophilic polymer with hydroxyl or amino groups into which acryloyl radicals are subsequently introduced or the carrier material is an aminated epoxypropyl methacrylate.
Claim 14
Dependent Claim
39 words
Adsorbent as claimed in claim 1 wherein the affine residue group carrying a free double bond is covalently bound to the polymeric carrier material in the presence of acrylamide by copolymerization via the free double bond of the poly-bisacrylamide.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-14 | 1985-12-14 | 1986-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-14 | 1989-12-14 | 1990-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-14 | 1993-12-14 | 1994-06-15 | $7700.00 | Window Closed / Lapsed |