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Patent US4334498A successfully refreshed from Google Patents.
US4334498A
Expired / Lapsed
US
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Assemblage for rearing nematodes
Assignee: Commonwealth Scientific and Industrial Research Organisation
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Filing: 1981-04-06
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Granted: 1982-06-15
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Expires: 2001-04-06
Abstract
Abstract An assemblage for rearing nematodes comprises a growth medium suitable for the culture of the nematodes, the growth medium being dispersed to allow free movement of fluids over the surface of the medium and to maximize the ratio of the surface area of the medium to volume of the assemblage. The assemblage may comprise an animal tissue homogenate as growth medium dispersed over the surface of elements of inert material such as wood-wool or crumbed plastics foam.
Inventors
- 👤 Robyn A. Bedding
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 10:47
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/30; A01K67/34; A01K67/36; A01K67/362; A01N; A01N63/00; A01N63/10; A01N63/12
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
53 words
A method for rearing nematodes comprising introducing a growth medium into a sealable container having a suitable support medium therein, sterilizing the contents of said container, inoculating said growth medium with a symbiotic bacteria for said nematodes, inoculating said growth medium with a nematode culture and incubating said nematode culture in said container.
Claim 2
Independent Claim
53 words
A method for rearing nematodes comprising introducing a growth medium into a sealable container having a suitable support medium therein, sterilizing the contents of said container, inoculating said growth medium with a symbiotic bacteria for said nematodes, inoculating said growth medium with a nematode culture and incubating said nematode culture in said container.
Claim 3
Dependent Claim
24 words
A method for rearing nematodes as set forth in claim 1, comprising deriving said symbiotic bacteria from the strain of nematodes to be cultured.
Claim 4
Dependent Claim
41 words
A method for rearing nematodes as set forth in claim 1, wherein said incubating is carried out by maintaining the inoculated growth medium in a moist aerated condition for a sufficient period of time to allow for multiplication of said nematodes.
Claim 5
Dependent Claim
39 words
A method for rearing nematodes as set forth in claim 1, further comprising the steps of harvesting said nematodes by washing said growth medium with sterile water or salt solution and filtering the harvested nematodes from the wash solution.
Claim 6
Dependent Claim
26 words
A method for rearing nematodes as set forth in claim 1, wherein said support medium is comprised of crumbed or broken up plastics, foam or sponge.
Claim 7
Dependent Claim
28 words
A method for rearing nematodes as set forth in claim 1, wherein said growth medium is comprised of an homogenate dispersed over the surface of said support medium.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-14 | 1985-12-14 | 1986-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-14 | 1989-12-14 | 1990-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-14 | 1993-12-14 | 1994-06-15 | $7700.00 | Window Closed / Lapsed |