US4332793A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of recovering cell antigen and preparation of feline leukemia vaccine therefrom
Assignee: Ohio State University Research Foundation
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Filing: 1979-12-18
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Granted: 1982-06-01
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Expires: 1999-12-18
Abstract
Abstract Disclosed is a vaccine which prevents disease caused by Feline leukemia virus (FeLV) and which comprises a protease enzyme-inhibited, cell-free, ostensibly virus-free, in vitro produced feline leukemia neoantigens including FOCMA which evokes an immune response in cats by the appearance of antibodies thereto and a FeLV virion gp70 neoantigen which evokes an immune response in cats by the appearance of antibodies thereto. A novel method for making the vaccine is disclosed also.
Inventors
- 👤 Richard G. Olson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 05:46
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61P; A61P31/00; A61P31/12; A61K; A61K39/00; C12; C12N; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/13011; C12N2740/13022; Y; Y10; Y10S; Y10S424/00; Y10S424/819
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
155 words
A vaccine for the prevention of disease caused by Feline leukemia virus (FeLV) which comprises: protease enzyme-inhibited, substantially virus-free, cell-free, in vitro produced feline leukemia neoantigens comprising (a) a FOCMA-type neoantigen which is capable of reading with FOCMA-antisera and which evokes an immune response in cats by the appearance of antibodies thereto; and (b) a FeLV virion gp70 neoantigen which is capable of reacting with gp70-antisera and which evokes an immune response in cats by the appearance of antibodies thereto, wherein said protease enzyme inhibition has been effected by at least one of the following techniques: (i) adding to said neoantigens an effective porportion of a protease enzyme inhibition; (ii) heating said neoantigens to a temperature effective to inhibit protease enzymes therein; (iii) changing the pH of said neoantigens to a value effective to inhibit protease enzymes therein; (iv) changing the ionic strength of said neoantigens to a value effective to inhibit protease enzymes therein.
Claim 2
Dependent Claim
155 words
A vaccine for the prevention of disease caused by Feline leukemia virus (FeLV) which comprises: protease enzyme-inhibited, substantially virus-free, cell-free, in vitro produced feline leukemia neoantigens comprising (a) a FOCMA-type neoantigen which is capable of reading with FOCMA-antisera and which evokes an immune response in cats by the appearance of antibodies thereto; and (b) a FeLV virion gp70 neoantigen which is capable of reacting with gp70-antisera and which evokes an immune response in cats by the appearance of antibodies thereto, wherein said protease enzyme inhibition has been effected by at least one of the following techniques: (i) adding to said neoantigens an effective porportion of a protease enzyme inhibition; (ii) heating said neoantigens to a temperature effective to inhibit protease enzymes therein; (iii) changing the pH of said neoantigens to a value effective to inhibit protease enzymes therein; (iv) changing the ionic strength of said neoantigens to a value effective to inhibit protease enzymes therein.
Claim 3
Dependent Claim
23 words
The vaccine of claim 1 wherein protease enzyme-inhibition is by the addition of an effective proportion of a protease inhibitor to said noeantigens.
Claim 4
Dependent Claim
31 words
The vaccine of claim 1 or 2 wherein said neoantigens further contain (c) a FeLV virion p27 antigen; (d) a FeLV virion p15 neoantigen; and (e) a FeLV virion p12 neoantigen.
Claim 5
Dependent Claim
13 words
The vaccine of claim 1 or 2 further comprising a pharmaceutically acceptable adjuvant.
Claim 6
Dependent Claim
23 words
A method for protecting a cat from Feline leukemia disease which comprises inoculated said cat with the vaccine of claim 1 or 2.
Claim 7
Dependent Claim
16 words
The method of claim 5 wherein said cat is inoculated with the vaccine of claim 3.
Claim 8
Dependent Claim
16 words
The method of claim 5 wherein said cat is inoculated with the vaccine of claim 4.
References Cited (Backward Citations)
US4086134A
Cited
DE2951509A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-31 | 1985-11-30 | 1986-06-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-31 | 1989-11-30 | 1990-06-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-31 | 1993-11-30 | 1994-06-01 | $7700.00 | Window Closed / Lapsed |