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US4330626A
Expired / Lapsed
US
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Method of preparing high-activity, low-bacteria, urease enzyme
Assignee: Enzyme Center Inc
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Filing: 1980-03-19
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Granted: 1982-05-18
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Expires: 2000-03-19
Abstract
Abstract In a method of preparing the enzyme, urease, from jack beans, wherein the jack beans are reduced to a fine particle size and the fine particles so reduced constitute urease, the fine jack-bean particles thereafter irradiated for a period of time, the irradiated jack beans then recovered and employed as a source of urease enzyme, the improvement which comprises: irradiating the whole jack beans prior to any size reduction of the seeds of the jack beans, to provide a urease derived from the white seeds of the irradiated whole jack beans having an improved activity level.
Inventors
- 👤 Henry E. Blair
- 👤 Stanley E. Charm
- 👤 Richard P. Crowley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 15:14
CPC Classifications
C; C12; C12N; C12N13/00; C12N9/00; C12N9/14; C12N9/78; C12N9/80
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
93 words
In a method of preparing the enzyme, urease, from jack beans, wherein the jack beans are reduced to a fine particle size and the fine jack-bean particles thereafter irradiated by gamma irradiation for a period of time, the irradiated jack beans then recovered and employed as a source of urease enzyme, the improvement which comprises: irradiating the whole jack beans with gamma irradiation prior to size reduction of the jack beans, to provide a urease having lower loss of activity than where the jack beans are first reduced in size and then irradiated.
Claim 2
Dependent Claim
93 words
In a method of preparing the enzyme, urease, from jack beans, wherein the jack beans are reduced to a fine particle size and the fine jack-bean particles thereafter irradiated by gamma irradiation for a period of time, the irradiated jack beans then recovered and employed as a source of urease enzyme, the improvement which comprises: irradiating the whole jack beans with gamma irradiation prior to size reduction of the jack beans, to provide a urease having lower loss of activity than where the jack beans are first reduced in size and then irradiated.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein the amount of gamma irradiation is from about 0.1 to 5.0 megarads.
Claim 4
Dependent Claim
35 words
The method of claim 1 wherein the specific activity of the urease is from about 170 to 300 based on the milligrams of ammonia liberated per gram of urease per 5 minutes at 25° C.
Claim 5
Dependent Claim
26 words
The method of claim 1 wherein the jack beans, having high levels of microbiological contamination, are removed prior to the size reduction of the jack beans.
Claim 6
Dependent Claim
22 words
The method of claim 1 wherein the irradiated jack beans are ground into a particle size of less than about 60 mesh.
Claim 7
Dependent Claim
112 words
In a method of preparing the enzyme, urease, from jack beans, wherein the jack beans are reduced to a fine particle size and the fine jack-bean particles thereafter irradiated by gamma irradiation for a period of time, the irradiated jack beans then recovered and employed as a source of urease enzyme, the improvement which comprises: irradiating the whole jack beans with from about 0.3 to 2.5 megarads of gamma-ray irradiation prior to size reduction of the jack beans to less than about 60 mesh, to provide a urease having a specific activity of from about 170 to 300 milligrams of ammonia liberated per gram of urease per 5 minutes at 25° C.
Claim 8
Dependent Claim
112 words
In a method of preparing the enzyme, urease, from jack beans, wherein the jack beans are reduced to a fine particle size and the fine jack-bean particles thereafter irradiated by gamma irradiation for a period of time, the irradiated jack beans then recovered and employed as a source of urease enzyme, the improvement which comprises: irradiating the whole jack beans with from about 0.3 to 2.5 megarads of gamma-ray irradiation prior to size reduction of the jack beans to less than about 60 mesh, to provide a urease having a specific activity of from about 170 to 300 milligrams of ammonia liberated per gram of urease per 5 minutes at 25° C.
References Cited (Backward Citations)
US3249513A
Cited
US4193845A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-17 | 1985-11-16 | 1986-05-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-17 | 1989-11-16 | 1990-05-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-17 | 1993-11-16 | 1994-05-18 | $7700.00 | Window Closed / Lapsed |