US4330528A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Drug for stimulating the rate of proliferation of liver cells and method for producing same
Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
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Filing: 1980-07-03
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Granted: 1982-05-18
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Expires: 2000-07-03
Abstract
Abstract A drug for stimulating the rate of proliferation of liver cells having as s active ingredient a blood plasma extract (NP) and a method of preparing such a drug wherein blood plasma is first acidified to a pH of 5.5, then heat denatured at approximately 95° C. and subsequently centrifuged to provide the extract.
Inventors
- 👤 Gerhard Ruhenstroth-Bauer
- 👤 Michel Goldberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 12:44
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61P; A61P1/00; A61P1/16; A61P43/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
96 words
A factor for stimulating the rate of proliferation of liver cells formed by a process comprising the steps of providing a quantity of blood plasma of normal animals. acidifying said blood plasma to a pH level of 5.5, heat denaturing said acidified plasma at a temperature of approximately 95° C., centrifuging said acidified and heat denatured blood plasma to remove the denatured proteins from the plasma, and treating the supernatant from said centrifugation with a peptide-peptidyl-hydrolase to obtain said factor, said factor being a neuraminic-acid-free and betagalactose-free peptide with a molecular weight of approximately 1200 D.
Claim 2
Dependent Claim
96 words
A factor for stimulating the rate of proliferation of liver cells formed by a process comprising the steps of providing a quantity of blood plasma of normal animals. acidifying said blood plasma to a pH level of 5.5, heat denaturing said acidified plasma at a temperature of approximately 95° C., centrifuging said acidified and heat denatured blood plasma to remove the denatured proteins from the plasma, and treating the supernatant from said centrifugation with a peptide-peptidyl-hydrolase to obtain said factor, said factor being a neuraminic-acid-free and betagalactose-free peptide with a molecular weight of approximately 1200 D.
Claim 3
Dependent Claim
16 words
A factor in accordance with claim 1, wherein said peptide-peptidyl hydrolases (NPTC) are trypsin and chymotrypsin.
Claim 4
Dependent Claim
97 words
A factor for stimulating the rate of proliferation of liver cells formed by a process comprising the steps of providing a quantity of blood plasma of normal animals, acidifying said blood plasma to a pH level of 5.5, heat denaturing said acidified plasma at a temperature of approximately 95° C., centrifuging said acidified and heat denatured blood plasma to remove the denatured protein from the plasma, and treating the supernatant from said centrifugation with trypsin or chymotrypsin to obtain said factor, said factor being a neuraminic-acid-free and betagalactose-free peptide with a molecular weight of approximately 1200 D.
Claim 5
Dependent Claim
97 words
A factor for stimulating the rate of proliferation of liver cells formed by a process comprising the steps of providing a quantity of blood plasma of normal animals, acidifying said blood plasma to a pH level of 5.5, heat denaturing said acidified plasma at a temperature of approximately 95° C., centrifuging said acidified and heat denatured blood plasma to remove the denatured protein from the plasma, and treating the supernatant from said centrifugation with trypsin or chymotrypsin to obtain said factor, said factor being a neuraminic-acid-free and betagalactose-free peptide with a molecular weight of approximately 1200 D.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-17 | 1985-11-16 | 1986-05-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-17 | 1989-11-16 | 1990-05-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-17 | 1993-11-16 | 1994-05-18 | $7700.00 | Window Closed / Lapsed |