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US4330463A
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Process of preparing blood cell protein from hemoglobin
Assignee: INTERNATIONALE OCTRODI "OCTROPA"BV ROCHUSSENSTRAAT 78 ROTTERDAM (THE NETHERLANDS) A Co OF NETHERLANDS Mij
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Filing: 1980-11-03
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Granted: 1982-05-18
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Expires: 2000-11-03
Abstract
Abstract Tasteless and odourless blood cell protein isolate and heme are prepared from hemoglobin by converting the hemoglobin into a solid form by dehydration and extracting the heme from the solid product by solvent extraction with an organic solvent, preferably methanol or a methanol/ethanol mixture or a methanol/ethanol/water mixture, in the presence of acid, preferably hydrochloric acid, and recovering the blood cell protein isolate from the extraction residue. The acid may also be added to the hemoglobin before its conversion into the solid form.
Inventors
- 👤 Jan H. Luijerink
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 13:20
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/06; A23K; A23K10/00; A23K10/20; A23K10/24; A23K20/00; A23K20/20; A23K20/30; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
86 words
A process of preparing bland blood cell protein isolate from hemoglobin, which comprise converting the hemoglobin into a solid form by dehydration, extracting the heme from this solid by solvent extraction with an organic solvent selected from the group consisting of (a) methanol, (b) a mixture of methanol and ethanol, and (c) a mixture of methanol, ethanol and water, said extraction being effected in the presence of sufficient acid to effect a pH below 3.5, and recovering the blood cell protein isolate as the extraction residue.
Claim 2
Dependent Claim
86 words
A process of preparing bland blood cell protein isolate from hemoglobin, which comprise converting the hemoglobin into a solid form by dehydration, extracting the heme from this solid by solvent extraction with an organic solvent selected from the group consisting of (a) methanol, (b) a mixture of methanol and ethanol, and (c) a mixture of methanol, ethanol and water, said extraction being effected in the presence of sufficient acid to effect a pH below 3.5, and recovering the blood cell protein isolate as the extraction residue.
Claim 3
Dependent Claim
20 words
A process according to claim 1, in which at least part of the acid is present in the organic solvent.
Claim 4
Dependent Claim
20 words
A process according to claim 1, in which at least part of the acid is present in the solid hemoglobin.
Claim 5
Dependent Claim
26 words
A process according to claim 1, in which the acid is added to the hemoglobin solution before conversion of the hemoglobin into solid form by dehydration.
Claim 6
Dependent Claim
15 words
A process according to claims 1,2,3 or 4, in which the acid is hydrochloric acid.
Claim 7
Dependent Claim
93 words
A process of preparing bland blood cell protein isolate from red blood cell concentrate, which comprises combining the red blood cell concentrate with a strong acid to pH value below 3.5 with vigorous stirring, converting the acidified solution into solid form by dehydration, separating the heme from this solid product by solvent extraction with an organic solvent selected from the group consisting of (a) methanol, (b) a mixture of methanol and ethanol, and (c) a mixture of methanol, ethanol and water, and recovering the bland blood cell protein isolate as the extraction residue.
Claim 8
Dependent Claim
93 words
A process of preparing bland blood cell protein isolate from red blood cell concentrate, which comprises combining the red blood cell concentrate with a strong acid to pH value below 3.5 with vigorous stirring, converting the acidified solution into solid form by dehydration, separating the heme from this solid product by solvent extraction with an organic solvent selected from the group consisting of (a) methanol, (b) a mixture of methanol and ethanol, and (c) a mixture of methanol, ethanol and water, and recovering the bland blood cell protein isolate as the extraction residue.
Claim 9
Dependent Claim
14 words
A process according to claim 6, in which the strong acid is hydrochloric acid.
Claim 10
Dependent Claim
89 words
A process of preparing bland blood cell protein isolate from red blood cell concentrate, which comprises spray-drying the red blood cell concentrate in the presence of sufficient gaseous hydrochloric acid to effect a pH below 3.5, extracting the heme from the solid, acid red blood cell concentrate thus obtained with an organic solvent selected from the group consisting of (a) methanol, (b) a mixture of methanol and ethanol, and (c) a mixture of methanol, ethanol and water, and recovering the bland blood cell protein isolate as the extraction residue.
Claim 11
Dependent Claim
89 words
A process of preparing bland blood cell protein isolate from red blood cell concentrate, which comprises spray-drying the red blood cell concentrate in the presence of sufficient gaseous hydrochloric acid to effect a pH below 3.5, extracting the heme from the solid, acid red blood cell concentrate thus obtained with an organic solvent selected from the group consisting of (a) methanol, (b) a mixture of methanol and ethanol, and (c) a mixture of methanol, ethanol and water, and recovering the bland blood cell protein isolate as the extraction residue.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-17 | 1985-11-16 | 1986-05-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-17 | 1989-11-16 | 1990-05-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-17 | 1993-11-16 | 1994-05-18 | $7700.00 | Window Closed / Lapsed |