US4329427A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fermentative preparation of L-isoleucine
Assignee: WR Grace and Co
•
Filing: 1980-10-06
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Granted: 1982-05-11
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Expires: 2000-10-06
Abstract
Abstract L-isoleucine is prepared by cultivation of an analogue-resistant mutant of Brevibacterium thiogenitalis in an aqueous nutrient medium under aerobic conditions in the presence of from 0.001 to 15 w/v percent of a post-threonine precursor for L-isoleucine. The cultivation is preferably carried out at about 30° C. and at a pH of 5 to 9. L-isoleucine is recovered from the fermentation broth.
Inventors
- 👤 Mark H. Updike
- 👤 Gary J. Calton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 20:39
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/06; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/13; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
105 words
A process for preparing L-isoleucine which comprises cultivating under aerobic conditions a mutant strain of Brevibacterium thiogenitalis resistant to an analogue of L-isoleucine selected from the group consisting of α-amino-β-hydroxyvaleric acid; methylglycine; gammadehydroisoleucine; 3-cyclopentene-1-glycine; 2-cyclopentene-1-glycine; o-methylthreonine; and β-hydroxyleucine, said cultivation carried out in the presence of a post-threonine biosynthetic precursor of L-isoleucine selected from the group consisting of α-hydroxy butyric acid, α-aceto-α-hydroxy butyric acid, α,β-dihydroxy-β-methylvaleric acid, α-keto butyric acid, and D,L-α-amino-n-butyric acid and alkali metal salts thereof, to yield a fermentation broth, accumulating from about 0.1 to about 6 w/v percent L-isoleucine in said fermentation broth, and recovering the accumulated L-isoleucine from said fermentation broth.
Claim 2
Dependent Claim
105 words
A process for preparing L-isoleucine which comprises cultivating under aerobic conditions a mutant strain of Brevibacterium thiogenitalis resistant to an analogue of L-isoleucine selected from the group consisting of α-amino-β-hydroxyvaleric acid; methylglycine; gammadehydroisoleucine; 3-cyclopentene-1-glycine; 2-cyclopentene-1-glycine; o-methylthreonine; and β-hydroxyleucine, said cultivation carried out in the presence of a post-threonine biosynthetic precursor of L-isoleucine selected from the group consisting of α-hydroxy butyric acid, α-aceto-α-hydroxy butyric acid, α,β-dihydroxy-β-methylvaleric acid, α-keto butyric acid, and D,L-α-amino-n-butyric acid and alkali metal salts thereof, to yield a fermentation broth, accumulating from about 0.1 to about 6 w/v percent L-isoleucine in said fermentation broth, and recovering the accumulated L-isoleucine from said fermentation broth.
Claim 3
Dependent Claim
14 words
A process as in claim 1 wherein the mutant is Brevibacterium thiogenitalis ATCC 31723.
Claim 4
Dependent Claim
24 words
A process as in claim 1 wherein the concentration of precursor in the media prior to fermentation is from 0.001 to 15 w/v percent.
Claim 5
Dependent Claim
27 words
A process as in claim 1 wherein the fermentation is carried out at a temperature of from 20° to 45° C. for from 8 to 176 hours.
Claim 6
Dependent Claim
20 words
A process as in claim 1 wherein the pH of the fermentation media during cultivation is from 5 to 9.
Claim 7
Dependent Claim
38 words
A process as in claim 1 wherein the mutant is Brevibacterium thiogenitalis ATCC 31723 and fermentation is carried out at from 20° to 45° C. for 8 to 176 hours at a pH of from 5 to 9.
Claim 8
Dependent Claim
19 words
A process as in claim 1 wherein the precursor is α-keto butyric acid or an alkali metal salt thereof.
Claim 9
Dependent Claim
18 words
A process as in claim 1 wherein the precursor is D,L-α-amino-n-butyric acid or an alkali metal salt thereof.
Claim 10
Dependent Claim
13 words
A process as in claim 1 wherein the analogue is α-amino-β-hydroxy valeric acid.
References Cited (Backward Citations)
US4237228A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-10 | 1985-11-09 | 1986-05-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-10 | 1989-11-09 | 1990-05-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-10 | 1993-11-09 | 1994-05-11 | $7700.00 | Window Closed / Lapsed |