US4237228A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of producing L-isoleucine using Brevibacterium flavum
Assignee: Individual
•
Filing: 1978-06-22
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Granted: 1980-12-02
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Expires: 1998-06-22
Abstract
Abstract A method of producing L-isoleucine which comprises culturing the strain of Brevibacterium flavum VNII Genetika 10-89 deposited under the number CMIM B-1507 in a nutrient medium containing sources of nitrogen, carbon, mineral salts and vitamins and under aerobic conditions. Said strain makes it possible to produce L-isoleucine with a yield of 17 g/l and more.
Inventors
- 👤 Nelli I. Zhdanova
- 👤 Tatyana V. Leonova
- 👤 Ljudmila F. Kozyreva
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 20:40
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/06; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
87 words
A method of producing L-isoleucine by direct fermentation of a producing strain comprising: (A) providing a nutrient medium consisting essentially of sources of carbon, nitrogen, mineral salts, vitamins, and water, with the proviso that precursors of L-isoleucine and amino acids are absent; (B) cultivating the strain of Brevibacterium flavum VNII Genetika 10-89 deposited under the number CMIM B-1507 in said medium under aerobic conditions until L-isoleucine is accumulated in an amount of at least 17 grams per liter; and (C) recovering the accumulated L-isoleucine from said medium.
Claim 2
Independent Claim
87 words
A method of producing L-isoleucine by direct fermentation of a producing strain comprising: (A) providing a nutrient medium consisting essentially of sources of carbon, nitrogen, mineral salts, vitamins, and water, with the proviso that precursors of L-isoleucine and amino acids are absent; (B) cultivating the strain of Brevibacterium flavum VNII Genetika 10-89 deposited under the number CMIM B-1507 in said medium under aerobic conditions until L-isoleucine is accumulated in an amount of at least 17 grams per liter; and (C) recovering the accumulated L-isoleucine from said medium.
Claim 3
Dependent Claim
22 words
The method of claim 1 wherein the strain of Brevibacterium flavum VNII Genetika 10-89 is resistant to the inhibiting effect of O-methylthreonine.
Claim 4
Dependent Claim
14 words
The method of claim 2 wherein the source of carbon is glucose or sacchrose.
Claim 5
Dependent Claim
28 words
The method of claim 3 wherein the vitamins are selected from at least one of the group consisting of biotin, desthiobiotin, thiamine and feed concentrate B 12 .
Claim 6
Dependent Claim
12 words
The method of claim 4 wherein the vitamins are biotin and thiamine.
Claim 7
Dependent Claim
16 words
The method of claim 4 wherein the vitamins are biotin and feed concentrate B 12 .
Claim 8
Dependent Claim
12 words
The method of claim 4 wherein the vitamins are thiamine and desthiobiotin.
Claim 9
Dependent Claim
16 words
The method of claim 4 wherein the vitamins are desthiobiotin and feed concentrate B 12 .
Claim 10
Dependent Claim
17 words
The method of claim 4 wherein the nitrogen source is (NH 4 ) 2 SO 4 .
Claim 11
Dependent Claim
21 words
The method of claim 9 wherein the mineral salts are KH 2 PO 4 , MgSO 4 and CaCO 3 .
References Cited (Backward Citations)
GB889187A
Cited
US3058888A
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US3262861A
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US3231478A
Cited
US3502544A
Cited
US3767529A
Cited
JPS516237A
Cited
JPS5121077A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-02 | 1984-06-02 | 1984-12-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-02 | 1988-06-02 | 1988-12-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-02 | 1992-06-02 | 1992-12-02 | $7700.00 | Window Closed / Lapsed |