Patent US4329152A successfully refreshed from Google Patents.
US4329152A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Determination of β2 -microglobulin in human urine and serum by latex immunoassay
Assignee: International Lead Zinc Research Organization Inc
•
Filing: 1980-10-06
•
Granted: 1982-05-11
•
Expires: 2000-10-06
Abstract
Abstract A highly sensitive method has been developed for the determination of β 2 -microglobulin in human urine or serum samples. This method is based on the agglutination of β 2 -microglobulin by latex particles coated with an anti-body against β 2 -microglobulin in a stabilizing solution.
Inventors
- 👤 Robert R. Lauwerys
- 👤 Alfred M. Bernard
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 15:52
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/68; G01N33/6893
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
90 words
A method for determining β 2 -microglobulin concentration in a human urine or serum sample comprising the steps of: (a) coating latex particles with an antibody against the β 2 -microglobulin; (b) incubating the antibody-coated particles diluted in a stabilizing solution with the sample so as to permit the β 2 -microglobulin to agglutinate a proportion of the particles, said proportion being related to the concentration of β 2 -microglobulin in the sample; and (c) quantitating the unagglutinated particles and thereby determining the β 2 -microglobulin concentration in the sample.
Claim 2
Independent Claim
90 words
A method for determining β 2 -microglobulin concentration in a human urine or serum sample comprising the steps of: (a) coating latex particles with an antibody against the β 2 -microglobulin; (b) incubating the antibody-coated particles diluted in a stabilizing solution with the sample so as to permit the β 2 -microglobulin to agglutinate a proportion of the particles, said proportion being related to the concentration of β 2 -microglobulin in the sample; and (c) quantitating the unagglutinated particles and thereby determining the β 2 -microglobulin concentration in the sample.
Claim 3
Dependent Claim
19 words
A method according to claim 1, wherein the stabilizing solution consists of glycine buffered saline containing bovine serum albumin.
Claim 4
Dependent Claim
16 words
A method according to claim 2, wherein said stabilizing solution is adjusted to about pH 10.
Claim 5
Dependent Claim
72 words
A method according to claim 1 or 2 wherein the latex particles are coated with antibody against the β 2 -microglobulin by the following steps: (a) diluting the antibody with glycine buffered saline; (b) adding the latex particles to the diluted antibody with agitation; and (c) precipitating the latex particles followed by the redispersement of the latex particles in glycine buffered saline; the resulting antibody-coated latex particles being suitable for long-term storage.
References Cited (Backward Citations)
US4162895A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-10 | 1985-11-09 | 1986-05-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-10 | 1989-11-09 | 1990-05-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-10 | 1993-11-09 | 1994-05-11 | $7700.00 | Window Closed / Lapsed |