Patent US4328309A successfully refreshed from Google Patents.
US4328309A
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Method for producing tripdiolide, triptolide and celastrol
Assignee: United States Department of Health and Human Services
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Filing: 1980-07-02
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Granted: 1982-05-04
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Expires: 2000-07-02
Abstract
Abstract A process for the production of tripdiolide, triptolide and celastrol comprises the steps of: (a) preparing a cellular inoculum from Tripterygium wilfordii Hook F; (b) inoculating a nutrient growth medium with the cellular inoculum and incubating the inoculated growth medium at 20°-30° C. for up to 8 weeks to produce a cellular product; (c) harvesting the cellular product from the inoculated growth medium; and (d) isolating tripdiolide, triptolide and celastrol from the cellular product and supernatant inoculated growth medium.
Inventors
- 👤 William T. Chalmers
- 👤 James P. Kutney
- 👤 Phillip J. Salisbury
- 👤 Kenneth L. Stuart
- 👤 Phillip M. Townsley
- 👤 Brian R. Worth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 18:43
CPC Classifications
C; C12; C12N; C12N1/00; C12P; C12P15/00; C12P17/00; C12P17/02; C12R; C12R2001/00; C12R2001/91
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
82 words
A process for obtaining tripdiolide, triptolide and celastrol comprising the steps of: (a) preparing a cellular inoculum from Tripteryguim wilfordii Hook F; (b) inoculating a nutrient growth medium with the cellular inoculum and incubating the inoculated growth medium at 20°-30° C. for up to 8 weeks to produce a cellular product at a pH below 7; (c) harvesting the cellular product from the inoculated growth medium; and (d) isolating tripdiolide, triptolide and celastrol from the cellular product and supernatant inoculated growth medium.
Claim 2
Dependent Claim
82 words
A process for obtaining tripdiolide, triptolide and celastrol comprising the steps of: (a) preparing a cellular inoculum from Tripteryguim wilfordii Hook F; (b) inoculating a nutrient growth medium with the cellular inoculum and incubating the inoculated growth medium at 20°-30° C. for up to 8 weeks to produce a cellular product at a pH below 7; (c) harvesting the cellular product from the inoculated growth medium; and (d) isolating tripdiolide, triptolide and celastrol from the cellular product and supernatant inoculated growth medium.
Claim 3
Dependent Claim
19 words
The process of claim 1, wherein the cellular inoculum is prepared from the leaves of T. wilfordii Hook F.
Claim 4
Dependent Claim
36 words
The process of claim 1, wherein preparation of the cellular inoculum and incubation of the cellular inoculum to produce cellular product is done in media containing sources of carbon, nitrogen, magnesium, calcium, phosphorus, sulfur and potassium;
Claim 5
Dependent Claim
10 words
The process of claim 3, wherein the media contain vitamins.
Claim 6
Dependent Claim
56 words
The process of claim 1, wherein preparation of the cellular inoculum is done in the presence of light in a medium containing a first phytohormone and coconut milk to produce a callus and wherein material from the callus is further incubated in the dark in a medium containing casein hydrolysate, coconut milk and a second phytohormone.
Claim 7
Dependent Claim
19 words
The process of claim 5, wherein the first phytohormone is indole-3-acetic acid and the second phytohormone is 2,4-dichlorophenoxyacetic acid.
Claim 8
Dependent Claim
35 words
The process of claim 1, wherein incubation of the cellular inoculum in the nutrient growth medium to produce cellular product is done under subdued light and the pH of the medium is maintained below 7.
Claim 9
Dependent Claim
13 words
The process of claim 7, wherein the nutrient growth medium contains coconut milk.
Claim 10
Dependent Claim
130 words
The process of claim 1, wherein the cellular inoculum is prepared from the leaves of T. wilfordii Hook F; wherein preparation of the cellular inoculum and incubation of the cellular inoculum to produce cellular product is done in media containing coconut milk, vitamins and sources of carbon, nitrogen, magnesium, calcium, phosphorus, sulfur and potassium; wherein preparation of the cellular inoculum is done in the presence of light in a medium containing indole-3-acetic acid to produce a callus and material from the callus is further incubated in the dark in a medium containing casein hydrolysate and 2,4-dichlorophenoxyacetic acid and wherein incubation of the cellular inoculum in the nutrient growth medium to produce cellular product is done under subdued light and the pH of the nutrient growth medium is maintained below 7.
References Cited (Backward Citations)
US4005108A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-03 | 1985-11-02 | 1986-05-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-03 | 1989-11-02 | 1990-05-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-03 | 1993-11-02 | 1994-05-04 | $7700.00 | Window Closed / Lapsed |