Patent US4320194A successfully refreshed from Google Patents.
US4320194A
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Process for the production of carrier particles from yeast cells and for producing diagnostic agents therefrom and test kits containing such agents
Assignee: Gist Brocades NV
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Filing: 1980-07-28
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Granted: 1982-03-16
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Expires: 2000-07-28
Abstract
Abstract Yeast cells are converted into carrier particles useful as carriers for proteins and other materials useful in agglutination tests by cross-linking the proteins of the cell cytoplasm followed by stabilization of the carbohydrates of the cell wall by reaction with an epoxide. The stabilized cells obtained may be dyed and may be esterified or etherified to block the hydroxyl groups in their surfaces. They may be coupled to the protein or other material by activation, e.g. with cyanuric chloride, followed by reaction with the protein or other material to produce diagnostic agents useful in test kits.
Inventors
- 👤 Frederick G. Bull
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 14:45
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/16; C12N1/00; C12N1/005; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/554; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/975
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
46 words
Process for the production of carrier particles from yeast cells which comprises cross-linking the proteins in the cell cytoplasm by reaction with a cross-linking agent and stabilizing the carbohydrate components of the yeast cell wall by reaction with an epihalohydrin or other low molecular weight epoxide.
Claim 2
Dependent Claim
46 words
Process for the production of carrier particles from yeast cells which comprises cross-linking the proteins in the cell cytoplasm by reaction with a cross-linking agent and stabilizing the carbohydrate components of the yeast cell wall by reaction with an epihalohydrin or other low molecular weight epoxide.
Claim 3
Dependent Claim
15 words
Process according to claim 1 in which the said cross-linking agent is formaldehyde or glutaraldehyde.
Claim 4
Dependent Claim
12 words
Process according to claim 1 in which the said epihalohydrin is epichlorohydrin.
Claim 5
Dependent Claim
14 words
Process according to claim 1 in which the said yeast cells are also dyed.
Claim 6
Dependent Claim
15 words
Process according to claim 4 in which the cells are dyed with a reactive dyestuff.
Claim 7
Dependent Claim
15 words
Process according to claim 4 in which the cells are dyed with a fluorescent dyestuff.
Claim 8
Dependent Claim
23 words
Process according to claim 1 in which the cells are treated so as to block the hydroxyl functions in the cell wall carbohydrates.
Claim 9
Dependent Claim
15 words
Process according to claim 7 in which the said hydroxyl functions are esterified or etherified.
Claim 10
Dependent Claim
18 words
Process according to claim 8 in which the said hydroxyl functions are acetylated, benzoylated, methylated, tetrahydropyranylated, or silylated.
Claim 11
Dependent Claim
34 words
Process for the production of a diagnostic reagent which comprises coupling yeast cells with an antibody, antigen, enzyme or lectin at any stage during a process as defined in claim 1, 4 or 7.
Claim 12
Dependent Claim
53 words
Process according to claim 10 in which the coupling takes place after the cross-linking of the proteins in the cell wall, the stabilization of the carbohydrate in the cell wall, and any optional dyeing of the yeast cells but before any optional treatment to block the hydroxyl functions of the cell wall carbohydrate.
Claim 13
Dependent Claim
50 words
Process according to claim 10 in which the coupling takes place after the cross-linking of the proteins in the cell wall, the stabilization of the carbohydrate in the cell wall, the treatment to block the hydroxyl functions of the cell wall carbohydrate, and any optional dyeing of the yeast cells.
Claim 14
Dependent Claim
35 words
Process according to claim 10 in which the yeast cells are first activated by reaction with cyanuric chloride and then coupled to the antibody, antigen, enzyme, or lectin by reaction of the activated cells therewith.
Claim 15
Dependent Claim
35 words
Process according to claim 10 in which the yeast cells are first activated by reaction with cyanogen bromide and then coupled to the antibody, antigen, enzyme or lectin by reaction of the activated cells therewith.
Claim 16
Dependent Claim
22 words
A test kit which comprises a water-soluble mannose oligomer and at least one diagnostic agent produced by the process of claim 10.
Claim 17
Dependent Claim
25 words
A test kit which comprises a water-soluble extract of yeast cell walls and at least one diagnostic agent produced by the process of claim 10.
Claim 18
Dependent Claim
10 words
Stabilized carrier particles produced by the process of claim 1.
Claim 19
Dependent Claim
10 words
A diagnostic reagent produced by the process of claim 10.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-03-15 | 1985-09-14 | 1986-03-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-03-15 | 1989-09-14 | 1990-03-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-03-15 | 1993-09-14 | 1994-03-16 | $7700.00 | Window Closed / Lapsed |