Patent US4317811A successfully refreshed from Google Patents.
US4317811A
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Herpes simplex type 1 subunit vaccine
Assignee: Merck and Co Inc
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Filing: 1980-09-11
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Granted: 1982-03-02
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Expires: 2000-09-11
Abstract
Abstract A herpes simplex type 1 subunit vaccine is prepared from infected chick embryo cells by urea-extraction of the virus while the infected cells are still attached to the growth surface.
Inventors
- 👤 Alexander U. Bertland
- 👤 George P. Lampson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:52
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/245; A61K2039/555; A61K2039/55505; C; C12; C12N; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16611; C12N2710/16634
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
47 words
A herpes simplex type 1 subunit vaccine prepared by the process comprising extracting cells infected with herpes simplex type 1 virus with urea while the cells are attached to the growth surface, clarifying the extract, concentrating the extract, and treating the extract to remove substantially all DNA.
Claim 2
Dependent Claim
47 words
A herpes simplex type 1 subunit vaccine prepared by the process comprising extracting cells infected with herpes simplex type 1 virus with urea while the cells are attached to the growth surface, clarifying the extract, concentrating the extract, and treating the extract to remove substantially all DNA.
Claim 3
Dependent Claim
21 words
A vaccine according to claim 1 wherein the urea has a concentration of from about 2 M to about 8 M.
Claim 4
Dependent Claim
16 words
A vaccine according to claim 1 wherein the urea extract is clarified by centrifugation or filtration.
Claim 5
Dependent Claim
14 words
A vaccine according to claim 1 wherein the clarified extract is concentrated by filtration.
Claim 6
Dependent Claim
18 words
A vaccine according to claim 1 wherein DNA is removed by treatment with an enzyme which degrades DNA.
Claim 7
Dependent Claim
11 words
A vaccine according to claim 5 wherein the enzyme is DNase.
Claim 8
Dependent Claim
16 words
A vaccine according to claim 1 wherein the extract after removal of DNA is gel chromatographed.
Claim 9
Dependent Claim
17 words
A vaccine according to claim 7 wherein the fractions at void volume are pooled and sterile filtered.
Claim 10
Dependent Claim
13 words
A vaccine according to claim 8 wherein the fractions are sonicated before filtering.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-03-01 | 1985-08-31 | 1986-03-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-03-01 | 1989-08-31 | 1990-03-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-03-01 | 1993-08-31 | 1994-03-02 | $7700.00 | Window Closed / Lapsed |