US4308232A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Anticoagulant stopper coating
Assignee: Covidien AG
•
Filing: 1979-07-09
•
Granted: 1981-12-29
•
Expires: 1999-07-09
Abstract
Abstract A stopper for a blood collection tube adapted to receive whole blood for centrifugal separation into its relatively light and heavy phases. The stopper is provided with a coating which prevents red blood cells from adhering to it so as to avoid red cell contamination of the lighter phase after phase separation. The stopper is coated with a cationic binding agent, such as tridodecylmethylammonium chloride, and then with an anionic anticoagulant such as dextran sulfate. A lubricant, such as silicone oil, is applied to the coated stopper to facilitate insertion of the stopper into the tube.
Inventors
- 👤 Ronald Crouther
- 👤 Fred E. Satchell
- 👤 Glen Stone
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 00:05
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/491; A; A61; A61L; A61L33/00; A61L33/0005; A61L33/0011; A61L33/0017; A61L33/06; A61L33/08; B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5021; B01L3/508; B01L3/5082; B01L3/50825; B01L2200/00; B01L2200/12; B01L2300/00; B01L2300/16; B01L2300/161; Y; Y10; Y10T; Y10T428/00; Y10T428/31504; Y10T428/31652; Y10T428/31663
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
73 words
A stopper for a blood collection container capable of sealingly closing one end of the container with a bottom surface thereof facing the opposed end of the container comprising an elastomeric closure member, a first layer of a binding agent covering said bottom surface, and a second layer including dextran sulfate bonded to and covering said first layer to inhibit adherence of red blood cells to said closure member when contacted by blood.
Claim 2
Dependent Claim
73 words
A stopper for a blood collection container capable of sealingly closing one end of the container with a bottom surface thereof facing the opposed end of the container comprising an elastomeric closure member, a first layer of a binding agent covering said bottom surface, and a second layer including dextran sulfate bonded to and covering said first layer to inhibit adherence of red blood cells to said closure member when contacted by blood.
Claim 3
Dependent Claim
13 words
The stopper of claim 1 wherein said binding agent is a cationic surfactant.
Claim 4
Dependent Claim
14 words
The stopper of claim 1 wherein said binding agent is a quaternary ammonium surfactant.
Claim 5
Dependent Claim
12 words
The stopper of claim 3 wherein said binding agent is tridodecylmethylammonium chloride.
Claim 6
Dependent Claim
18 words
The stopper of claim 1 further including a layer of a lubricant covering at least said second layer.
Claim 7
Dependent Claim
26 words
The stopper of claim 1 wherein the stopper includes a plug portion having an annular sealing surface, and a lubricant on at least said sealing surface.
Claim 8
Dependent Claim
37 words
The stopper of claim 1 wherein said binding agent is a quaternary ammonium salt, said binding agent layer covers substantially the entire surface of said member, and said second layer covers substantially the entire binding agent layer.
Claim 9
Dependent Claim
86 words
In a blood collection device including a blood collection tube, and a stopper closing one end of the tube, the improvement comprising a first layer of a cationic surfactant binding agent on at least the bottom surface of the stopper within the tube, a second layer including dextran sulfate covering said first layer and being bound to said first layer such that it is substantially insoluble in whole blood, said second layer inhibiting adherence of red blood cells to the stopper during use of the device.
Claim 10
Dependent Claim
86 words
In a blood collection device including a blood collection tube, and a stopper closing one end of the tube, the improvement comprising a first layer of a cationic surfactant binding agent on at least the bottom surface of the stopper within the tube, a second layer including dextran sulfate covering said first layer and being bound to said first layer such that it is substantially insoluble in whole blood, said second layer inhibiting adherence of red blood cells to the stopper during use of the device.
Claim 11
Dependent Claim
12 words
The device of claim 8 wherein said first layer includes tridodecylmethylammonium chloride.
Claim 12
Dependent Claim
17 words
The device of claim 1 or 9 wherein the stopper has a lubricant coating said second layer.
Claim 13
Dependent Claim
12 words
The device of claim 10 wherein said lubricant is a silicone oil.
Claim 14
Dependent Claim
33 words
The device of claim 1 or 9 wherein said first layer covers substantially the entire outer surface of the stopper, said second layer covers substantially the entire outer surface of said first layer.
Claim 15
Dependent Claim
18 words
The device of claim 12 wherein a lubricant covers substantially the entire outer surface of said second layer.
Claim 16
Dependent Claim
29 words
The device of claim 13 wherein the tube has a partial vacuum therein, and the stopper is pierceable by a needle cannula for introducing whole blood into the tube.
Claim 17
Dependent Claim
14 words
The device of claim 14 wherein the stopper comprises a material including butyl rubber.
Claim 18
Dependent Claim
35 words
The stopper of claim 1 or 8 wherein said second layer has a heparin activity that substantially does not effect an increase the clotting time of blood when disposed in the container during use thereof.
Claim 19
Independent Claim
104 words
The method of treating an elastomeric closure stopper for a blood collection container to inhibit the adhesion of red blood cells thereto during use comprising the steps of coating at least that portion of the stopper that is subject to come in contact with blood with a solution of a binding agent, drying the stopper to effect a dry binding agent layer, coating the binding agent layer with a solution including dextran sulfate, and drying the stopper to effect a dried layer including dextran sulfate bonded to the binding agent layer, to inhibit red blood cell adhesion to the stopper when contacted by blood.
Claim 20
Independent Claim
104 words
The method of treating an elastomeric closure stopper for a blood collection container to inhibit the adhesion of red blood cells thereto during use comprising the steps of coating at least that portion of the stopper that is subject to come in contact with blood with a solution of a binding agent, drying the stopper to effect a dry binding agent layer, coating the binding agent layer with a solution including dextran sulfate, and drying the stopper to effect a dried layer including dextran sulfate bonded to the binding agent layer, to inhibit red blood cell adhesion to the stopper when contacted by blood.
Claim 21
Dependent Claim
18 words
The method of claim 17 wherein said binding agent and said dextran sulfate are cationic and anionic, respectively.
Claim 22
Dependent Claim
14 words
The method of claim 17 wherein said binding agent includes a quaternary ammonium salt.
Claim 23
Dependent Claim
12 words
The method of claim 19 wherein said binding agent is tridodecylmethylammonium chloride.
Claim 24
Dependent Claim
23 words
The method of claim 17 or 20 further including the step of applying a coating of a lubricant over said dextran sulfate layer.
Claim 25
Dependent Claim
21 words
The method of claim 17 wherein the binding agent solution includes a quaternary ammonium salt and a solvent for said salt.
Claim 26
Dependent Claim
29 words
The method of claim 22 wherein the binding agent solution comprises the quaternary ammonium salt in a range between about 0.1 to about 0.8 grams per milliliter of solvent.
Claim 27
Dependent Claim
25 words
The method of claim 23 wherein about 0.3 grams of quaternary ammonium chloride per milliliter of solvent is used to form the binding agent solution.
Claim 28
Dependent Claim
13 words
The method of claim 24 wherein the quaternary ammonium chloride is tridodecylmethylammonium chloride.
Claim 29
Dependent Claim
13 words
The method of claim 25 wherein the solvent includes petroleum ether and toluene.
Claim 30
Dependent Claim
17 words
The method of claim 26 wherein the solvent has approximately equal amounts of petroleum ether and toluene.
Claim 31
Dependent Claim
29 words
The method of claim 17 or 22 wherein the solution including dextran sulfate has heparin units of activity which are more than 15 units and less than 160 units.
Claim 32
Dependent Claim
22 words
The method of claim 17 wherein said dried dextran sulfate layer is aged in an oven at a temperature above the ambient.
Claim 33
Dependent Claim
14 words
The method of claim 17 wherein said solution including dextran sulfate further includes water.
Claim 34
Dependent Claim
20 words
The method of claim 30 further including the step of applying a lubricant to cover the dried dextran sulfate layer.
Claim 35
Dependent Claim
29 words
The method of claim 31 wherein each of the steps of applying said binding agent solution and said solution including dextran sulfate include immersing the stopper in said solutions.
Claim 36
Dependent Claim
18 words
The method of claim 17 wherein the solution including dextran sulfate has approximately 80 heparin units of activity.
References Cited (Backward Citations)
US3634123A
Cited
US3617344A
Cited
US3673612A
Cited
US3810781A
Cited
US3882021A
Cited
US3958572A
Cited
US4118485A
Cited
US4116898A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-12-28 | 1985-06-29 | 1985-12-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-12-28 | 1989-06-29 | 1989-12-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-12-28 | 1993-06-29 | 1993-12-29 | $7700.00 | Window Closed / Lapsed |