US4307188A
Expired / Lapsed
US
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Precursor indicator compositions
Assignee: Bayer Corp
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Filing: 1979-09-06
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Granted: 1981-12-22
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Expires: 1999-09-06
Abstract
Abstract A composition, test device, method of making a test device and process for determination of an analyte in a liquid sample are disclosed. More particularly, there is provided a composition for detecting an analyte in a liquid sample comprising an analyte-responsive component and an indicator composition having a plurality of components comprising at least one nonresponsive precursor component and at least one component effective in a liquid milieu to alter said precursor component so as to release at least one substance effective to permit said indicator composition, in combination with said analyte-responsive component, to produce a detectable response to said analyte. The composition can optionally be incorporated with a carrier to form a device.
Inventors
- 👤 William I. White
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 06:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q1/34; C12Q1/42; C12Q1/44; C12Q1/46; C12Q1/54; C12Q1/61; G01N31/00; G01N31/22; C12Q2326/00; C12Q2326/32; C12Q2334/00; C12Q2334/30; C12Q2334/50; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
79 words
A composition for detecting an analyte in a liquid sample comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to cleave the ester or amide linkage so as to release a substance effective to permit said indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte.
Claim 2
Dependent Claim
79 words
A composition for detecting an analyte in a liquid sample comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to cleave the ester or amide linkage so as to release a substance effective to permit said indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte.
Claim 3
Dependent Claim
36 words
The composition of claim 1 wherein the nonresponsive precursor component is indoxyl acetate and the component effective to alter said nonresponsive precursor component is a catalyst capable of cleaving the acetate group from the indoxyl acetate.
Claim 4
Dependent Claim
17 words
The composition of claim 2 wherein the component effective to alter said nonresponsive precursor component is cholinesterase.
Claim 5
Dependent Claim
17 words
The composition of claim 2 wherein the component effective to alter said nonresponsive precursor component is imidazole.
Claim 6
Dependent Claim
56 words
The composition of claim 1 wherein the nonresponsive precursor component is a compound having the structure ##STR8## in which Y is lower alkyl carbonyl, lower alkoxy carbonyl or a phosphate ester of the structure ##STR9## in which R and R' are independently H or lower alkyl, and, each X is independently selected from bromine and chlorine.
Claim 7
Dependent Claim
40 words
The composition of claim 5 wherein the component effective to alter said nonresponsive precursor component is an esterase when Y is alkyl carbonyl, a carbonic anhydrase when Y is lower alkoxy carbonyl or a phosphatase when Y is phosphate ester.
Claim 8
Dependent Claim
26 words
The composition of claim 1 wherein the nonresponsive precursor component is a compound having the structure ##STR10## in which R is independently hydrogen or lower alkyl.
Claim 9
Dependent Claim
17 words
The composition of claim 7 wherein the component effective to alter said nonresponsive precursor component is β-glucuronidase.
Claim 10
Dependent Claim
34 words
The composition of claim 1 wherein the indicator comprises a component resulting from the alteration of the precursor component and an interreactive coupling compound selected from the group consisting of 3-loweralkykl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinonehydrazone.
Claim 11
Dependent Claim
12 words
The composition of claim 9 wherein the nonresponsive precursor component is (γ-glutamyl)-1-naphthylamine.
Claim 12
Dependent Claim
18 words
The composition of claim 10 wherein the component effective to alter said nonresponsive precursor component is γ-glutamyl transferase.
Claim 13
Dependent Claim
37 words
The composition of claim 9 wherein the nonresponsive precursor component is a lower peptide ester having the structure ##STR11## in which R and R' are independently hydrogen, alkyl, alkoxy, halogen, or together form an aromatic hydrocarbon ring.
Claim 14
Dependent Claim
18 words
The composition of claim 12 wherein the component effective to alter said nonresponsive precursor component is an esterase.
Claim 15
Dependent Claim
27 words
The composition of claim 9 wherein the nonresponsive precursor component is a component having the structure ##STR12## wherein R and R' are independently H or lower alkyl.
Claim 16
Dependent Claim
18 words
The composition of claim 14 wherein the component effective to alter said nonresponsive precursor component is a phosphatase.
Claim 17
Dependent Claim
26 words
The composition of claim 9 wherein the nonresponsive precursor component is a compound having the structure ##STR13## wherein R is H, lower alkyl, or lower alkoxy.
Claim 18
Dependent Claim
17 words
The composition of claim 16 wherein the component effective to alter said nonresponsive precursor component is β-glucosidase.
Claim 19
Dependent Claim
24 words
The composition of claim 9 wherein the nonresponsive precursor component is a compound having the structure ##STR14## wherein R is H or lower alkyl.
Claim 20
Dependent Claim
17 words
The composition of claim 18 wherein the component effective to alter said nonresponsive precursor component is β-glucuronidase.
Claim 21
Independent Claim
101 words
A device for detecting a sample analyte which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte.
Claim 22
Independent Claim
101 words
A device for detecting a sample analyte which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte.
Claim 23
Independent Claim
142 words
A device for detecting a sample analyte which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage; (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; (3) a component resulting from the alteration of the precursor component; and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone.
Claim 24
Independent Claim
142 words
A device for detecting a sample analyte which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage; (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; (3) a component resulting from the alteration of the precursor component; and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone.
Claim 25
Dependent Claim
119 words
A process for preparing a device for detecting a sample analyte which comprises the steps of: (a) incorporating a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage; (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; and thereafter (b) drying the carrier which has been so incorporated.
Claim 26
Dependent Claim
119 words
A process for preparing a device for detecting a sample analyte which comprises the steps of: (a) incorporating a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage; (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; and thereafter (b) drying the carrier which has been so incorporated.
Claim 27
Dependent Claim
161 words
A process for preparing a device for detecting a sample analyte which comprises the steps of: (a) incorporating a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage; (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; (3) a component resulting from the alteration of the precursor component; and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone; and thereafter (b) drying the carrier which has been so incorporated.
Claim 28
Dependent Claim
161 words
A process for preparing a device for detecting a sample analyte which comprises the steps of: (a) incorporating a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage; (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; (3) a component resulting from the alteration of the precursor component; and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone; and thereafter (b) drying the carrier which has been so incorporated.
Claim 29
Independent Claim
92 words
A method for detecting an analyte in a sample which comprises the steps of: (a) contacting the sample with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a component effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; and thereafter (b) observing any detectable response.
Claim 30
Independent Claim
92 words
A method for detecting an analyte in a sample which comprises the steps of: (a) contacting the sample with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a component effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; and thereafter (b) observing any detectable response.
Claim 31
Independent Claim
138 words
A method for detecting an analyte in a sample which comprises the steps of: (a) contacting the sample with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; (3) a component resulting from the alteration of the precursor component; and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone; and thereafter (b) observing any detectable response.
Claim 32
Independent Claim
138 words
A method for detecting an analyte in a sample which comprises the steps of: (a) contacting the sample with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; (3) a component resulting from the alteration of the precursor component; and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone; and thereafter (b) observing any detectable response.
Claim 33
Independent Claim
122 words
A method for detecting an analyte in a sample which comprises the steps of: (a) contacting the sample with a device which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; and thereafter (b) observing any detectable response.
Claim 34
Independent Claim
122 words
A method for detecting an analyte in a sample which comprises the steps of: (a) contacting the sample with a device which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage and (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; and thereafter (b) observing any detectable response.
Claim 35
Independent Claim
163 words
A method for detecting an analyte in a sample which comprises the steps of: (a) contacting the sample with a device which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage; (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; (3) a component resulting from the alteration of the precursor component; and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone; and thereafter (b) observing any detectable response.
Claim 36
Independent Claim
163 words
A method for detecting an analyte in a sample which comprises the steps of: (a) contacting the sample with a device which comprises a carrier, which carrier is insoluble in and maintains its structural integrity when exposed to the sample to be tested, incorporated with a composition comprising an analyte-responsive component and an indicator composition comprising (1) a nonresponsive precursor component having an ester or amide linkage; (2) a hydrolytic catalyst in an amount effective in a liquid milieu to alter the nonresponsive precursor component so as to release a substance effective to permit the indicator composition, through reaction with a product of the analyte-responsive component, to produce a detectable response to the analyte; (3) a component resulting from the alteration of the precursor component; and (4) a coupling compound, which is interreactive with the component resulting from the alteration of the precursor component and selected from the group consisting of 3-loweralkyl-2-benzothiazolinone hydrazone and 1-loweralkyl-2-quinolinone hydrazone; and thereafter (b) observing any detectable response.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-12-21 | 1985-06-22 | 1985-12-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-12-21 | 1989-06-22 | 1989-12-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-12-21 | 1993-06-22 | 1993-12-22 | $7700.00 | Window Closed / Lapsed |