Patent US4302544A successfully refreshed from Google Patents.
US4302544A
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Asporogenous mutant of B. subtilis for use as host component of HV1 system
Assignee: University of Rochester
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Filing: 1979-10-15
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Granted: 1981-11-24
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Expires: 1999-10-15
Abstract
Abstract Asporogenous mutant B. subtilis RUB 331 (ATCC 31578) and a process for using such mutant are disclosed. The asporogenous mutant desirably reverts to sporeformers with a frequency not greater than 10-7 reversions per bacterium per generation and meets the requirements of the NIH "Guidelines for Research Involving Recombinant DNA Molecules" for a B. subtilis host component of a Host-Vector 1 system.
Inventors
- 👤 Frank E. Young
- 👤 Gary A. Wilson
- 👤 Susan L. Mottice
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 05:17
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/75; C12R; C12R2001/00; C12R2001/01; C12R2001/07; C12R2001/125; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
96 words
A biologically pure culture of asporgenous B. subtilis RUB 331 (ATCC 31578) suitable for use as a host component in a host-vector system having the following characteristics: translucent phenotype on tryptose blood agar plates but not on Spizizen's minimal agar supplemented with glucose; a frequency of transformation with linear or covalently closed circular DNA of up to 2 percent; lyses in a complex medium; viability reduced to 0 CFU/ml after drying at room temperature for about 12 hours and a frequency of reversion to sporeformers of less than 10 -7 , under conditions of minimal aeration.
Claim 2
Dependent Claim
96 words
A biologically pure culture of asporgenous B. subtilis RUB 331 (ATCC 31578) suitable for use as a host component in a host-vector system having the following characteristics: translucent phenotype on tryptose blood agar plates but not on Spizizen's minimal agar supplemented with glucose; a frequency of transformation with linear or covalently closed circular DNA of up to 2 percent; lyses in a complex medium; viability reduced to 0 CFU/ml after drying at room temperature for about 12 hours and a frequency of reversion to sporeformers of less than 10 -7 , under conditions of minimal aeration.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-23 | 1985-05-25 | 1985-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-23 | 1989-05-25 | 1989-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-23 | 1993-05-25 | 1993-11-24 | $7700.00 | Window Closed / Lapsed |