US4302533A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Novel assays for interferon
Assignee: Yeda Research and Development Co Ltd
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Filing: 1980-04-11
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Granted: 1981-11-24
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Expires: 2000-04-11
Abstract
Abstract The present invention relates to an assay for the quantitative determination of interferon which comprises extracting a cell previously exposed to said interferon with an extractant, and determining in such extract the quantity of an enzyme the content of which in said cell is a function of the quantity of interferon to which said cell had been previously exposed and to a kit for carrying out such assay.
Inventors
- 👤 Michel Revel
- 👤 Adi Kimchi
- 👤 Lester Shulman
- 👤 David Wallach
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 13:05
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6863; G01N33/6866; C; C12; C12Q; C12Q1/00; C12Q1/25; C12Q1/34; C12Q1/44; C12Q1/48; G01N2333/00; G01N2333/435; G01N2333/52; G01N2333/555; Y; Y10; Y10S; Y10S435/00; Y10S435/811
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
72 words
An assay for the quantitative determination of interferon, without the necessity of viral infection, consisting essentially of the steps of extracting a cell previously exposed to said interferon by means of a non-ionic surfactant, and determining in such extract the quantity of protein kinase (PK-i), oligo-isoadenylate synthetase, or phosphodiesterase, the content of which in said cell is a function of the quantity of interferon to which said cell has been previously exposed.
Claim 2
Dependent Claim
72 words
An assay for the quantitative determination of interferon, without the necessity of viral infection, consisting essentially of the steps of extracting a cell previously exposed to said interferon by means of a non-ionic surfactant, and determining in such extract the quantity of protein kinase (PK-i), oligo-isoadenylate synthetase, or phosphodiesterase, the content of which in said cell is a function of the quantity of interferon to which said cell has been previously exposed.
Claim 3
Dependent Claim
64 words
An assay according to claim 1, further including, prior to said extractant step, adding a substrate, which is to be assayed as to its interferon content, to a cell culture and using said cell culture, after a predetermined period of time, as said cells being extracted, the quantity of enzyme determined by said determining step being indicative of the interferon content of the substrate.
Claim 4
Dependent Claim
22 words
An assay according to claim 2 wherein the cell culture used is one of leukocytes, lymphocytes, human fibroblasts, or mouse L cells.
Claim 5
Dependent Claim
24 words
An assay according to claim 1 or 2 wherein the enzyme assay is carried out by incorporation of radioactive markers and determination of these.
Claim 6
Dependent Claim
26 words
An assay according to claim 2, wherein the cells are extracted after from 3 to 24 hours of exposure of the cell culture to said substrate.
References Cited (Backward Citations)
US4061538A
Cited
US3910824A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-23 | 1985-05-25 | 1985-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-23 | 1989-05-25 | 1989-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-23 | 1993-05-25 | 1993-11-24 | $7700.00 | Window Closed / Lapsed |