Patent US4302386A successfully refreshed from Google Patents.
US4302386A
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Antigenic modification of polypeptides
Assignee: The Ohio State University
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Filing: 1980-01-16
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Granted: 1981-11-24
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Expires: 2000-01-16
Abstract
Abstract Modified hormones or fragments of hormones are useful in producing antibodies when administered to an animal. Said antibodies in turn cause neutralization of endogenous natural protein hormones. The modification may be accomplished by attaching various kinds of modifying groups to the hormone or fragment. Modification may, for example, be achieved by chemically coupling diazosulfanilic acid groups to the hormone or fragment. The protein hormones to which this procedure can be applied are mammalian protein reproductive hormones such as, for example, Follicle Stimulating Hormone (FSH), or Human Chorionic Gonadotropin (HGG). These modified hormone or fragment may be administered to animals for the purpose of contraception, abortion, or treatment of hormone related disease states and disorders.
Inventors
- 👤 Vernon C. Stevens
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:29
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/0005; A61K39/0006; A61K2039/555; A61K2039/55511; A61K2039/5555; A61K2039/60; A61K2039/6012; A61K38/00; Y; Y10; Y10S; Y10S424/00; Y10S424/811; Y10S930/00; Y10S930/01; Y10S930/11
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
128 words
A modified polypeptide for isoimmuniogically controlling biological action in a mammal by antibody formation, consisting of a protein hormone, a non-hormonal protein, or a fragment of either which has been chemically modified outside the body of said mammal, said protein hormone, non-hormonal protein or fragment having the properties of: (a) in unmodified form, being non-immunogenic to said mammal and having a moleculr structure similar to an endogenous protein hormone or a non-hormonal protein, the biological function of which it is desired to inhibit, or fragment of either and (b) in modified form, causing antibodies to be formed in the body of the mammal which inhibit the biological function of said endogenous protein hormone or non-hormonal protein following administration of the modified form into the body of said mammal.
Claim 2
Dependent Claim
128 words
A modified polypeptide for isoimmuniogically controlling biological action in a mammal by antibody formation, consisting of a protein hormone, a non-hormonal protein, or a fragment of either which has been chemically modified outside the body of said mammal, said protein hormone, non-hormonal protein or fragment having the properties of: (a) in unmodified form, being non-immunogenic to said mammal and having a moleculr structure similar to an endogenous protein hormone or a non-hormonal protein, the biological function of which it is desired to inhibit, or fragment of either and (b) in modified form, causing antibodies to be formed in the body of the mammal which inhibit the biological function of said endogenous protein hormone or non-hormonal protein following administration of the modified form into the body of said mammal.
Claim 3
Dependent Claim
25 words
A modified polypeptide according to claim 1 to which there has been chemically added 1-40 modifying groups per protein hormone, non-hormnal protein, or fragment thereof.
Claim 4
Dependent Claim
25 words
A modified polypeptide according to claim 1 to which there has been chemically added 5-30 modifying groups per protein hormone, non-hormonal protein, or fragment thereof.
Claim 5
Dependent Claim
25 words
A modified polypeptide according to claim 1 to which there has been chemically added 10-26 modifying groups per protein hormone, non-hormonal protein, or fragment thereof.
Claim 6
Dependent Claim
16 words
A modified polypeptide according to claim 1 wherein said polypeptide is a natural protein reproductive hormone.
Claim 7
Dependent Claim
17 words
A modified polypeptide according to claim 1 wherein said polypeptide is FSH, HCG, LH, HPL, or prolactin.
Claim 8
Dependent Claim
18 words
A modified polypeptide according to claim 1 wherein said polypeptide is gastrin, angiotension II, growth hormone, or somatomedian.
Claim 9
Dependent Claim
18 words
A modified polypeptide according to claim 1 wherein said polypeptide is HCG, FSH, or a beta subunit thereof.
Claim 10
Dependent Claim
16 words
A modified polypeptide according to claim 3 wherein said polypeptide is the beta subunit of HCG.
Claim 11
Dependent Claim
16 words
A modified polypeptide according to claim 4 wherein said polypeptide is the beta subunit of HCG.
Claim 12
Dependent Claim
16 words
A modified polypeptide according to claim 2 wherein said polypeptide is the beta subunit of FSH.
Claim 13
Dependent Claim
22 words
A modified polypeptide according to claim 1 wherein said polypeptide is of the chemical configuration: Asp-His-Pro-Leu-Thr-Aba-Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Ser-Ser-Ser-Ser-Lys-Als-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln-Pro-Pro-Pro-Pro-Pro-Pro-Cys; Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln-Pro-Pro-Pro-Pro-Pro-Pro-Cys; Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln-Cys; Thr-Cys-Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln; Asp-His-Pro-Leu-Thr-Aba-Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln-Cys; or Cys-Pro-Pro-Pro-Pro-Pro-Pro-Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln.
Claim 14
Dependent Claim
24 words
A modified polypeptide according to claim 1 wherein said polypeptide is of the chemical configuration: Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln; Gln-Aso-Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln; Cys-Pro-Pro-Pro-Pro-Pro-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln, Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Pro-Pro-Pro-Pro-Pro-Pro-Cys; Phe-Gln-Asp-Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln; Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Ser-Ser-Ser-Lys-Als-Pro-Pro-Pro-Ser-Leu-Pro-Ser; Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Pro-Pro-Pro-Cys-Pro-Pro-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln; or Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Pro-Pro-Pro-Pro-Pro-Pro-Cys-Pro-Pro-Pro-Pro-Pro-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln.
Claim 15
Dependent Claim
26 words
A modified polypeptide according to claim 1 wherein said polypeptide comprises an 8-41 amino acid chain consisting of the C-terminal residues of an HCG beta subunit.
Claim 16
Dependent Claim
26 words
A modified polypeptide according to claim 1 wherein said polypeptide comprises a 20-44 amino acid chain consisting of the C-terminal residues of an HCG beta subunit.
Claim 17
Dependent Claim
34 words
A modified polypeptide according to claim 1 wherein said polypeptide is modified by addition of at least one of a diazosulfanilic acid, dinitrophenol, trinitrophenol, S-acetomercaptosuccinic anhydride, (poly)tyrosine, (poly)alanine, (poly)dextran, thyroglobulin, or a combination thereof.
Claim 18
Dependent Claim
22 words
A modified polypeptide according to claim 1 wherein said polypeptide is modified by addition of a (poly)tryosine, (poly)alanine or a mixture thereof.
Claim 19
Dependent Claim
18 words
A modified polypeptide according to claim 1 wherein said polypeptide is modified by addition of a diazosulfanilic acid.
Claim 20
Dependent Claim
34 words
A modified polypeptide according to claim 6 wherein said polypeptide is modified by addition of at least one of a diazosulfanilic acid, dinitrophenol, trinitrophenol, S-acetomercaptosuccinic anhydride, (poly)tyrosine, (poly)analine, (poly)dextran, thryoglobulin, or a mixture thereof.
Claim 21
Dependent Claim
34 words
A modified polypeptide according to claim 7 wherein said polypeptide is modified by addition of at least one of a diazosulfanilic acid, dinitrophenol, trinitrophenol, S-acetomercaptosuccinic anhydride, (poly)tyrosine, (poly)alanine, (poly)dextran, thryoglobulin, or a mixture thereof.
Claim 22
Dependent Claim
18 words
A modified polypeptide according to claim 8 wherein said polypeptide is modified by addition of a diazosulfanilic acid.
Claim 23
Dependent Claim
18 words
A modified polypeptide according to claim 10 wherein said polypeptide is modified by addition of a diazosulfanilic acid.
Claim 24
Dependent Claim
18 words
A modified polypeptide according to claim 11 wherein said polypeptide is modified by addition of a diazosulfanilic acid.
Claim 25
Dependent Claim
18 words
A modified polypeptide according to claim 12 wherein said polypeptide is modified by addition of a diazosulfanilic acid.
Claim 26
Dependent Claim
39 words
A modified polypeptide according to claim 1 wherein said polypeptide is modified by addition of diazosulfanilic acid, dinitrophenol, trinitrophenol, S-acetomercaptosuccinic anhydride, (poly)tyrosine, (poly)alanine, (poly)dextran, thyroglobulin, biodegradable polydextran, natural proteins, polymerized sugars, serum protein, a virus or a mixture thereof.
Claim 27
Dependent Claim
19 words
A modified polypeptide according to claim 1 wherein said polypeptide is modified by addition of sucrose copolymerized with epichlorohydrin.
Claim 28
Dependent Claim
39 words
A modified polypeptide according to claim 8 wherein said polypeptide is modified by addition of diazosulfanilic acid dinitrophenol, trinitrophenol, S-acetomercaptosuccinic anhydride, (poly)tyrosine, (poly)alanine, (poly)dextran, thyroglobulin, biodegradable polydextran, natural proteins, polymerized sugar, serum protein, a virus or a mixture thereof.
Claim 29
Dependent Claim
19 words
A modified polypeptide according to claim 8 wherein said polypeptide is modified by addition of sucrose copolymerized with epichlorohydrin.
Claim 30
Dependent Claim
39 words
A modified polypeptide according to claim 12 wherein said polypeptide is modified by addition of diazosulfanilic acid, dinitrophenol, trinitrophenol, S-acetomercaptosuccinic anhydride, (poly)tyrosine, (poly)alanine, (poly)dextran, thyroglobulin, biodegradable polydextran, natural proteins, polymerized sugar, serum protein, a virus or a mixture thereof.
Claim 31
Dependent Claim
39 words
A modified polypeptide according to claim 13 wherein said polypeptide is modified by addition of diazosulfanilic acid, dinitrophenol, trinitrophenol, S-acetomercaptosuccinic anhydride, (poly)tyrosine, (poly)alanine, (poly)dextran, thyroglobulin, biodegradable polydextran, natural proteins, polymerized sugars, serum protein, a vuris or a mixture thereof.
Claim 32
Dependent Claim
13 words
A modified polypeptide according to claim 25 wherein said virus is diphtheria virus.
Claim 33
Dependent Claim
17 words
A modified polypeptide according to claim 6 wherein said polypeptide is modified by addition of diphtheria toxoid.
Claim 34
Dependent Claim
16 words
A modified polypeptide according to claim 1 wherein said polypeptide is HCG or a sub-unit thereof.
Claim 35
Dependent Claim
12 words
A modified polypeptide according to claim 33 wherein said sub-unit is Thr-Cys-Asp-Asp-Pro-Arg-Phe-Gln-Asp-Ser-Ser-Ser-Ser-Lys-Ala-Pro-Pro-Pro-Ser-Leu-Pro-Ser-Pro-Ser-Arg-Leu-Pro-Gly-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln.
Claim 36
Dependent Claim
19 words
A modified polypeptide according to claim 33 or 34 wherein said polypeptide is modified by addition of diptheria toxoid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-23 | 1985-05-25 | 1985-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-23 | 1989-05-25 | 1989-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-23 | 1993-05-25 | 1993-11-24 | $7700.00 | Window Closed / Lapsed |