US4302384A
Expired / Lapsed
US
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Purification of gammaglobulin derivative
Assignee: Chemo Sero Therapeutic Research Institute Kaketsuken
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Filing: 1979-11-30
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Granted: 1981-11-24
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Expires: 1999-11-30
Abstract
Abstract Process for the production of a highly purified S-sulfonated gammaglobulin by treating an S-sulfonated gammaglobulin obtained by sulfonating a conventional gammaglobulin with an ion exchanger in a buffer solution for development, and thereby absorbing single molecular S-sulfonated gammaglobulin thereto, and eluting the single molecular S-sulfonated gammaglobulin with a buffer solution for elution. This process can give the desired S-sulfonated gammaglobulin having a high content of single molecule and an extremely small anticomplementary activity (e.g. CH50 of 10% or less) and also an excellent shaking stability from the conventional gammaglobulin in an industrial scale.
Inventors
- 👤 Akinobu Funatsu
- 👤 Shuzoh Oyama
- 👤 Yoshinori Akimoto
- 👤 Komei Ohashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 12:43
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/06; C07K16/065; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
67 words
A process for the production of a purified S-sulfonated gammaglobulin which comprises treating an S-sulfonated gammaglobulin with an anion exchanger in a buffer solution for development having a pH level of 7 to 8 and an ionic strength of 0.01 to 0.15 and thereby absorbing single molecular S-sulfonated gammaglobulin on the anion exchanger, and then eluting the single molecular S-sulfonated gammaglobulin with a buffer solution for elution.
Claim 2
Dependent Claim
67 words
A process for the production of a purified S-sulfonated gammaglobulin which comprises treating an S-sulfonated gammaglobulin with an anion exchanger in a buffer solution for development having a pH level of 7 to 8 and an ionic strength of 0.01 to 0.15 and thereby absorbing single molecular S-sulfonated gammaglobulin on the anion exchanger, and then eluting the single molecular S-sulfonated gammaglobulin with a buffer solution for elution.
Claim 3
Dependent Claim
71 words
A process according to claim 1, wherein the anion exchanger is a member selected from the group consisting of agarose gel introduced with an anionic substituent, dextran gel introduced with an anionic substituent, cellulose gel introduced with an anionic substituent, polyvinyl gel introduced with an anionic substituent, and amylose gel introduced with an anionic substituent, said anionic substituent being a member selected from the group consisting of diethylaminoethyl, triethylaminoethyl and diethyl-(2-hydroxypropyl)aminoethyl.
Claim 4
Dependent Claim
43 words
A process according to claim 1 or 2 therefor, wherein a buffer solution having a pH level of 3 to 4 and an ionic strength of higher than that of the buffer solution for development is used as the buffer solution for elution.
Claim 5
Dependent Claim
24 words
A process according to claim 3, wherein the ionic strength of the buffer solution for elution is in the range of 0.05 to 0.8.
Claim 6
Dependent Claim
67 words
A process for the production of a purified S-sulfonated gammaglobulin which comprises treating an S-sulfonated gammaglobulin with a cation exchanger in a buffer solution for development having a pH level of 4 to 6.5 and an ionic strength of 0.01 to 0.1 and thereby absorbing single molecular S-sulfonated gammaglobulin onto the cation exchanger, and then eluting the single molecular S-sulfonated gammaglobulin with a buffer solution for elution.
Claim 7
Dependent Claim
67 words
A process for the production of a purified S-sulfonated gammaglobulin which comprises treating an S-sulfonated gammaglobulin with a cation exchanger in a buffer solution for development having a pH level of 4 to 6.5 and an ionic strength of 0.01 to 0.1 and thereby absorbing single molecular S-sulfonated gammaglobulin onto the cation exchanger, and then eluting the single molecular S-sulfonated gammaglobulin with a buffer solution for elution.
Claim 8
Dependent Claim
71 words
A process according to claim 5, wherein the cation exchanger is a member selected from the group consisting of agarose gel introduced with a cationic substituent, dextran gel introduced with a cationic substituent, cellulose gel introduced with a cationic substituent, polyvinyl gel introduced with a cationic substituent, and amylose gel introduced with a cationic substituent, said cationic substituent being a member selected from the group consisting of carboxymethyl, sulfopropyl and sulfoethyl.
Claim 9
Dependent Claim
46 words
A process according to any one of claim 5, 8 or 9, wherein a buffer solution having a pH level of 6 to 9 and an ionic strength of higher than that of the buffer solution for development is used as the buffer solution for elution.
Claim 10
Dependent Claim
24 words
A process according to claim 7, wherein the ionic strength of the buffer solution for development is in the range of 0.05 to 0.8.
Claim 11
Dependent Claim
49 words
A process for separating single molecules from aggregated molecules of S-sulfonated gammaglobulin which comprises preferentially absorbing the said single molecules on an ion exchanger from a buffered solution having a pH which is compatable with the ion exchanger and eluting the ion exchanger to remove the absorbed gamma globulin.
Claim 12
Dependent Claim
49 words
A process for separating single molecules from aggregated molecules of S-sulfonated gammaglobulin which comprises preferentially absorbing the said single molecules on an ion exchanger from a buffered solution having a pH which is compatable with the ion exchanger and eluting the ion exchanger to remove the absorbed gamma globulin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-23 | 1985-05-25 | 1985-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-23 | 1989-05-25 | 1989-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-23 | 1993-05-25 | 1993-11-24 | $7700.00 | Window Closed / Lapsed |