US4301115A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Test device resistant to cross contamination between reactant areas and process for making it
Assignee: Bayer Corp
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Filing: 1979-06-22
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Granted: 1981-11-17
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Expires: 1999-06-22
Abstract
Abstract A test device for detecting the presence of constituents in a liquid test sample, and method for preparing it are disclosed. The device comprises a base support member coated with a hydrophobic barrier layer onto which is affixed a plurality of spaced apart reagents respectively responsive to different constituents of the test sample. The barrier layer comprises finely divided silica particles, to the surface of which are randomly covalently bound groups having the structure -O-SiR3 wherein the R substituents, same or different, are hydrogen, lower alkyl or aryl.
Inventors
- 👤 Myron C. Rapkin
- 👤 David L. Tabb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 11:56
CPC Classifications
G; G01; G01N; G01N31/00; G01N31/22; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
94 words
In a test device for analyzing a test sample for the presence of one or more constituents, the device comprising a base support member, a hydrophobic layer affixed to the support member, and two or more reagent compositions affixed to the hydrophobic layer, each respectively responsive to a particular sample constituent or concentration thereof, the improvement wherein said hydrophobic layer comprises finely divided silica particles having covalently affixed to the surfaces thereof groups having the structure --O--SiR.sub.3 wherein said R substituents, same or different, are hydrogen, lower alkyl, or aryl; and a suitable binder.
Claim 2
Dependent Claim
94 words
In a test device for analyzing a test sample for the presence of one or more constituents, the device comprising a base support member, a hydrophobic layer affixed to the support member, and two or more reagent compositions affixed to the hydrophobic layer, each respectively responsive to a particular sample constituent or concentration thereof, the improvement wherein said hydrophobic layer comprises finely divided silica particles having covalently affixed to the surfaces thereof groups having the structure --O--SiR.sub.3 wherein said R substituents, same or different, are hydrogen, lower alkyl, or aryl; and a suitable binder.
Claim 3
Dependent Claim
13 words
The improvement of claim 1 wherin the R substituents are all lower alkyl.
Claim 4
Dependent Claim
12 words
The improvement of claim 1 wherein the R substituents are all methyl.
Claim 5
Dependent Claim
26 words
The improvement of any of claims 1-3 wherein each reagent is incorporated, respectively, with a hydrophilic carrier matrix, each matrix being affixed to the hydrophobic layer.
Claim 6
Dependent Claim
26 words
The improvement of any of claims 1-3 wherein each reagent, respectively, is incorporated with a paper carrier matrix, each matrix being affixed to the hydrophobic layer.
Claim 7
Dependent Claim
14 words
The improvement of any of claims 1-3 wherein the binder is an acrylic polymer.
Claim 8
Dependent Claim
41 words
The improvement of claim 1 wherein the base support member is a polystyrene film, the R substituents in the hydrophobic layer are lower alkyl, the binder is an acrylic polymer, and the reagent compositions are incorporated, respectively, with hydrophilic carrier matrices.
Claim 9
Dependent Claim
26 words
The improvement of claim 7 wherein the reagent compositions are responsive, respectively, to albumin, bilirubin, glucose, hydrogen ion, ketone, nitrite, occult blood, urobilinogen or combinations thereof.
Claim 10
Dependent Claim
21 words
The improvement of claim 8 wherein reagent compositions are additionally provided which ae responsive to ascorbic acid, specific gravity or both.
Claim 11
Independent Claim
118 words
A method for preparing a test device capable of determining the presence of a constituent in a test sample wherein the device has the property of eliminating the occurrence of runover, the method comprising the steps of preparing a hydrophobic coating material comprising (a) finely divided silica particles having covalently affixed thereto groups having the structure -O-SiR 3 , wherein the R substituents, same or different, are hydrogen, lower alkyl or aryl, and (b) a suitable binder, affixing the hydrophobic coating material to a base support member to form a hydrophobic layer on the support member, and affixing to the hydrophobic layer a reagent capable of producing a detectable response in the presence of the test sample constituent.
Claim 12
Independent Claim
118 words
A method for preparing a test device capable of determining the presence of a constituent in a test sample wherein the device has the property of eliminating the occurrence of runover, the method comprising the steps of preparing a hydrophobic coating material comprising (a) finely divided silica particles having covalently affixed thereto groups having the structure -O-SiR 3 , wherein the R substituents, same or different, are hydrogen, lower alkyl or aryl, and (b) a suitable binder, affixing the hydrophobic coating material to a base support member to form a hydrophobic layer on the support member, and affixing to the hydrophobic layer a reagent capable of producing a detectable response in the presence of the test sample constituent.
Claim 13
Dependent Claim
22 words
The method of claim 10 wherein said reagent is incorporated with a hydrophilic carrier matrix which is affixed to said hydrophobic layer.
Claim 14
Dependent Claim
22 words
The method of claim 10 wherein said reagent is incorporated with a paper carrier matrix which is affixed to said hydrophobic layer.
Claim 15
Dependent Claim
41 words
The method of claim 10 which includes the additional steps of affixing to the hydrophobic layer additional reagents respectively responsive to other constituents of a test sample, said reagents being affixed to the hydrophobic layer in spaced relation to each other.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-16 | 1985-05-18 | 1985-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-16 | 1989-05-18 | 1989-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-16 | 1993-05-18 | 1993-11-17 | $7700.00 | Window Closed / Lapsed |