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Patent US4294866A successfully refreshed from Google Patents.
US4294866A
Expired / Lapsed
US
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Method for producing monocellular layers of cell-containing biological fluid
Assignee: Bayer Corp
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Filing: 1980-11-06
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Granted: 1981-10-13
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Expires: 2000-11-06
Abstract
Abstract An improved method is provided for producing monocellular layers of cell-containing biological fluid, such as blood, by spinning a sample slide bearing a sample to be analyzed. In the practice of the improved method, the slide is positioned with the sample-bearing surface thereof facing downwardly so that the force of gravity aids, rather than hinders, monocellular layer formation. Such layer formation is further facilitated by spinning of the slide in a holder provided with integral wall means shielding the sample from the buffeting effect of circumambient air to thereby avoid premature drying of the sample, minimize cell distortion and prevent aerosol formation.
Inventors
- 👤 Leighton C. Johnson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:06
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/28; G01N1/2813
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
64 words
A method of producing a monocellular layer of cell-containing biological fluid on a microscope slide or the like, which method comprises the steps of depositing a sample of the fluid on a surface of a microscope slide or the like, inverting said slide so that the sample-bearing surface thereof faces downwardly, and then spinning said slide about a vertical axis normal to said surface.
Claim 2
Independent Claim
64 words
A method of producing a monocellular layer of cell-containing biological fluid on a microscope slide or the like, which method comprises the steps of depositing a sample of the fluid on a surface of a microscope slide or the like, inverting said slide so that the sample-bearing surface thereof faces downwardly, and then spinning said slide about a vertical axis normal to said surface.
Claim 3
Dependent Claim
23 words
The method of claim 1 wherein, prior to spinning, the sample is spread over said surface of the slide by the wedge technique.
Claim 4
Dependent Claim
11 words
The method of claim 1 wherein said biological fluid is blood.
Claim 5
Dependent Claim
19 words
The method of claim 1 wherein said sample is prevented from contact by buffeting air currents during said spinning.
Claim 6
Dependent Claim
31 words
The method of claim 1 wherein said slide is spun while in a holder therefor having wall means for preventing contact of said sample by buffeting air currents during said spinning.
Claim 7
Dependent Claim
35 words
The method of claim 1 wherein said slide is spun while in a holder therefor and sample portions spun off from said slide during spinning are collected in well means forming part of said holder.
Claim 8
Dependent Claim
50 words
The method of claim 1 wherein said slide is spun while in a holder therefor having wall means for preventing contact of said sample by buffeting air currents during spinning, and sample portions spun off from said slide during spinning are collected in well means forming part of said holder.
Claim 9
Independent Claim
63 words
A method of producing a monocellular layer of a cell-containing biological fluid on a microscope slide or the like, which method comprises the steps of depositing a sample of the fluid on a surface of microscope slide or the like, and then spinning said slide about a vertical axis normal to said surface while preventing contact of said sample by buffeting air currents.
Claim 10
Independent Claim
63 words
A method of producing a monocellular layer of a cell-containing biological fluid on a microscope slide or the like, which method comprises the steps of depositing a sample of the fluid on a surface of microscope slide or the like, and then spinning said slide about a vertical axis normal to said surface while preventing contact of said sample by buffeting air currents.
Claim 11
Dependent Claim
23 words
The method of claim 8 wherein, prior to spinning, the sample is spread over said surface of the slide by the wedge technique.
Claim 12
Dependent Claim
11 words
The method of claim 8 wherein said biological fluid is blood.
Claim 13
Dependent Claim
31 words
The method of claim 8 wherein said slide is spun while in a holder therefor having wall means for preventing contact of said sample by buffeting air currents during said spinning.
Claim 14
Dependent Claim
35 words
The method of claim 8 wherein said slide is spun while in a holder therefor and sample portions spun off from said slide during spinning are collected in well means forming part of said holder.
Claim 15
Dependent Claim
51 words
The method of claim 8 wherein said slide is spun while in a holder therefor having wall means for preventing contact of said sample by buffeting air currents during said spinning, and sample portions spun off from said slide during spinning are collected in well means forming part of said holder.
References Cited (Backward Citations)
US3705048A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-12 | 1985-04-13 | 1985-10-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-12 | 1989-04-13 | 1989-10-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-12 | 1993-04-13 | 1993-10-13 | $7700.00 | Window Closed / Lapsed |