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US4292162A
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Buffer composition and method for the electrophoretic separation of proteins
Assignee: Pall Corp
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Filing: 1980-10-16
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Granted: 1981-09-29
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Expires: 2000-10-16
Abstract
Abstract A buffer composition for use in the electrophoretic separation of proteins into fractions, the buffer composition consisting essentially of Tris; an acid having the formula R 1 --CO--NH--R 2 --COOH where R 1 is NH 2 or an alkyl or aryl group, preferably NH 2 , C 6 H 5 or C 6 H 4 NH 2 and where R 2 is an alkyl group, preferably CH 2 , CH 2 --CH 2 or CH(CH 3 ); and a water soluble salt of the acid; the acid and salt being present in amounts and in a ratio to maintain the pH of an aqueous solution of the composition at from 8.2 to 9.0. The preferred acid is hippuric acid.
Inventors
- 👤 Jeffrey Ambler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 22:41
CPC Classifications
B; B01; B01D; B01D57/00; B01D57/02; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/108331
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
30 words
A composition for the preparation of a buffer solution for use in the electrophoretic separation of proteins, said composition consisting essentially of 2-amino-2-(hydroxymethyl)-1,3-propanediol, hippuric acid and a water soluble hippurate.
Claim 2
Dependent Claim
30 words
A composition for the preparation of a buffer solution for use in the electrophoretic separation of proteins, said composition consisting essentially of 2-amino-2-(hydroxymethyl)-1,3-propanediol, hippuric acid and a water soluble hippurate.
Claim 3
Dependent Claim
16 words
A composition as set forth in claim 1 which additionally includes a water soluble inorganic salt.
Claim 4
Dependent Claim
41 words
A composition as set forth in claim 1 wherein specified ingredients are present in amounts sufficient to provide the solution with a 2-amino-2-(hydroxymethyl)-1,3-propanediol concentration of from about 5 to 8 grams per liter, and a pH of from 8.2 to 9.0.
Claim 5
Dependent Claim
18 words
A composition as set forth in claim 1 wherein the water soluble hippurate is an alkali metal hippurate.
Claim 6
Dependent Claim
73 words
A composition as set forth in claim 1 wherein the Tris is present in an amount to provide a concentration thereof from 5 to 8 grams per liter, wherein the hippurate is present in an amount to provide a concentration thereof of from 6 to 10 grams per liter and wherein the hippuric acid is present in an amount to provide a concentration thereof of from one-fourth to one-half that of the hippurate.
Claim 7
Dependent Claim
49 words
A composition as set forth in claim 1 containing the specified ingredients in amounts sufficient to provide the solution with a 2-amino-2-(hydroxymethyl)-1,3-propanediol concentration of about 6 grams per liter, a hippurate concentration of about 8 grams per liter and a hippuric acid concentration of about 2.5 grams per liter.
Claim 8
Dependent Claim
32 words
A buffer solution for use in the electrophoretic separation of proteins, said buffer solution being made with a composition set forth in any of claims 1 2, 3, 4, 5 or 6.
Claim 9
Dependent Claim
63 words
A composition for the preparation of a buffer solution for use in the electrophoretic separation of proteins, said composition consisting essentially of 2-amino-2-(hydroxymethyl)-1,3-propanediol; an acid having the formula R 1 --CO--NH--R 2 --COOH where R 1 is NH 2 , an alkyl group or an aryl group and where R 2 is an alkyl group; and a water soluble salt of the acid.
Claim 10
Dependent Claim
63 words
A composition for the preparation of a buffer solution for use in the electrophoretic separation of proteins, said composition consisting essentially of 2-amino-2-(hydroxymethyl)-1,3-propanediol; an acid having the formula R 1 --CO--NH--R 2 --COOH where R 1 is NH 2 , an alkyl group or an aryl group and where R 2 is an alkyl group; and a water soluble salt of the acid.
Claim 11
Dependent Claim
42 words
A composition as set forth in claim 8 wherein R 1 is NH 2 , C 6 H 5 or C 6 H 4 NH 2 and where R 2 is CH 2 , CH 2 --CH 2 or CH(CH 3 ).
Claim 12
Dependent Claim
16 words
A composition as set forth in claim 8 which additionally includes a water soluble inorganic salt.
Claim 13
Dependent Claim
42 words
A composition as set forth in claim 8 wherein the specified ingredients are present in amounts sufficient to provide the solution with a 2-amino-2-(hydroxymethyl)-1,3-propanediol concentration of from about 5 to 8 grams per liter, and a pH of from 8.2 to 9.0.
Claim 14
Dependent Claim
18 words
A composition as set forth in claim 8 wherein the water soluble salt is an alkali metal salt.
Claim 15
Dependent Claim
82 words
A composition as set forth in claim 8 wherein the 2-amino-2-(hydroxymethyl)-1,3-propanediol is present in an amount to provide a concentration thereof within a range of from 5 to 8 grams per liter, and wherein the concentration of the sum of the acid and the salt is from about 0.03 to 0.08 moles per liter, the ratio of the acid and salt and the concentration of the 2-amino-2-(hydroxymethyl)-1,3-propanediol within said range being such as to provide a pH of from 8.2 to 9.0.
Claim 16
Dependent Claim
13 words
A composition as set forth in claim 8 wherein the acid is N-benzoyl-α-alanine.
Claim 17
Dependent Claim
13 words
A composition as set forth in claim 8 wherein the acid is N-benzoyl-β-alanine.
Claim 18
Dependent Claim
14 words
A composition as set forth in claim 8 wherein the acid is hydantoic acid.
Claim 19
Dependent Claim
14 words
A composition as set forth in claim 8 wherein the acid is p-aminohippuric acid.
Claim 20
Dependent Claim
62 words
An aqueous electrically conductive solution for use as a buffer solution in the electrophoretic separation of proteins, said aqueous solution containing 2-amino-2-(hydroxymethyl)-1,3-propanediol, an acid having the formula R 1 --CO--NH--R 2 --COOH where R 1 is NH 2 , an alkyl group or an aryl group and where R 2 is an alkyl group; and a water soluble salt of the acid.
Claim 21
Dependent Claim
62 words
An aqueous electrically conductive solution for use as a buffer solution in the electrophoretic separation of proteins, said aqueous solution containing 2-amino-2-(hydroxymethyl)-1,3-propanediol, an acid having the formula R 1 --CO--NH--R 2 --COOH where R 1 is NH 2 , an alkyl group or an aryl group and where R 2 is an alkyl group; and a water soluble salt of the acid.
Claim 22
Dependent Claim
42 words
An aqueous solution set forth in claim 18 wherein R 1 is NH 2 , C 6 H 5 or C 6 H 4 NH 2 and where R 2 is CH 2 , CH 2 --CH 2 or CH(CH 3 ).
Claim 23
Dependent Claim
17 words
An aqueous solution as set forth in claim 18 which additionally includes a water soluble inorganic salt.
Claim 24
Dependent Claim
41 words
An aqueous solution as set forth in claim 18 wherein the specified ingredients present in amounts sufficient to provide the solution with 2-amino-2-(hydroxymethyl)-1,3-propanediol concentration of from about 5 to 8 grams per liter, and a pH of from 8.2 to 9.0.
Claim 25
Dependent Claim
83 words
An aqueous solution as set forth in claim 18 wherein the a-amino-2-(hydroxymethyl)-1,3-propanediol is present in an amount to provide a concentration thereof within a range of from 5 to 8 grams per liter, and wherein the concentration of the sum of the acid and the salt is from about 0.03 to 0.08 moles per liter, the ratio of the acid and salt and the concentration of the a-amino-2-(hydroxymethyl)-1,3-propanediol within said range being such as to provide a pH of from 8.2 to 9.0.
Claim 26
Dependent Claim
14 words
An aqueous solution as set forth in claim 18 wherein the acid is N-benzoyl-α-alanine.
Claim 27
Dependent Claim
14 words
An aqueous solution as set forth in claim 18 wherein the acid is N-benzoyl-β-alanine.
Claim 28
Dependent Claim
15 words
An aqueous solution as set forth in claim 18 wherein the acid is hydantoic acid.
Claim 29
Dependent Claim
15 words
An aqueous solution as set forth in claim 18 wherein the acid is p-aminohippuric acid.
References Cited (Backward Citations)
US4243749A
Cited
US4246084A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-28 | 1985-03-30 | 1985-09-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-28 | 1989-03-30 | 1989-09-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-28 | 1993-03-30 | 1993-09-29 | $7700.00 | Window Closed / Lapsed |