Patent US4292161A successfully refreshed from Google Patents.
US4292161A
Expired / Lapsed
US
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Vertical gel slab electrophoresis apparatus
Assignee: Hoefer Scientific Instruments
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Filing: 1980-09-22
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Granted: 1981-09-29
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Expires: 2000-09-22
Abstract
Abstract A vertical gel electrophoresis apparatus includes a U-shaped structure consisting of a container for upper buffer solution and extending parallel plate sample units which form the legs of the U. In the casting mode the U-shaped structure is inverted with the tank resting on the ground surface and with the bottom ends of the sample units being sealed against the sealing strip on the top of the container. After the sample units are filled to the top and the gel polymerized (with the use of a comblike well forming structure if needed) the sample units are flipped and reinserted. However because of the offset of the plates of the sample units in the lateral direction the formerly sealed end of the sample plates is now exposed to a slot or aperture which extends into the upper buffer solution container. The entire U-shaped structure rests in a tank of lower buffer solution on a slab type heat exchanger where cooling can occur on both sides of the sample units.
Inventors
- 👤 Peter S. Hoefer
- 👤 Michael Whitesides
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 12:06
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
156 words
Vertical gel slab electrophoresis apparatus comprising: a sample unit comprising, a pair of spaced plates, a pair of vertical guide means each having an internal groove for accommodating opposed edges of said spaced plates each of said guide means having at least one external horizontal channel, and a plurality of screw means in said vertical guide means for pressing said plates together and against said internal grooves; means for supporting said sample unit in a vertical orientation; longitudinal resilient sealing means interposed between said sample unit and said supporting means; said supporting means including opposed means extending from said supporting means for receiving said pair of vertical guide means, a pair of cam means mounted for rotation in said extending means, said cam means having an off-center nub which mates with said horizontal channel of each vertical guide means, rotation of said cam means pressing said sample unit against said sealing means and said supporting means.
Claim 2
Dependent Claim
156 words
Vertical gel slab electrophoresis apparatus comprising: a sample unit comprising, a pair of spaced plates, a pair of vertical guide means each having an internal groove for accommodating opposed edges of said spaced plates each of said guide means having at least one external horizontal channel, and a plurality of screw means in said vertical guide means for pressing said plates together and against said internal grooves; means for supporting said sample unit in a vertical orientation; longitudinal resilient sealing means interposed between said sample unit and said supporting means; said supporting means including opposed means extending from said supporting means for receiving said pair of vertical guide means, a pair of cam means mounted for rotation in said extending means, said cam means having an off-center nub which mates with said horizontal channel of each vertical guide means, rotation of said cam means pressing said sample unit against said sealing means and said supporting means.
Claim 3
Dependent Claim
16 words
Apparatus as in claim 1 including a pair of first vertical longitudinal spacers between said plates.
Claim 4
Dependent Claim
27 words
Apparatus as in claim 2 including a pair of second vertical spacers between a plate and said screw means, such screw means pressing against said second spacers.
Claim 5
Dependent Claim
18 words
Apparatus as in claim 1 where said horizontal channels are located at end portions of said guide means.
Claim 6
Dependent Claim
15 words
Apparatus as in claim 1 where said supporting means forms an upper buffer solution container.
Claim 7
Dependent Claim
124 words
Vertical gel slab electrophoresis apparatus comprising: a sample unit including a pair of spaced plates held together at opposed edges by a pair of vertical guide means each having at least one external horizontal channel; means for supporting said sample unit in a vertical orientation; longitudinal resilient sealing means interposed between said sample unit and said supporting means; said supporting means including opposed means extending from said supporting means for receiving said pair of vertical guide means, a pair of cam means mounted for rotation in said extending means, said cam means having an off-center nub which mates with said horizontal channel of each vertical guide means, rotation of said cam means pressing said sample unit against said sealing means and said supporting means.
Claim 8
Dependent Claim
124 words
Vertical gel slab electrophoresis apparatus comprising: a sample unit including a pair of spaced plates held together at opposed edges by a pair of vertical guide means each having at least one external horizontal channel; means for supporting said sample unit in a vertical orientation; longitudinal resilient sealing means interposed between said sample unit and said supporting means; said supporting means including opposed means extending from said supporting means for receiving said pair of vertical guide means, a pair of cam means mounted for rotation in said extending means, said cam means having an off-center nub which mates with said horizontal channel of each vertical guide means, rotation of said cam means pressing said sample unit against said sealing means and said supporting means.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-28 | 1985-03-30 | 1985-09-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-28 | 1989-03-30 | 1989-09-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-28 | 1993-03-30 | 1993-09-29 | $7700.00 | Window Closed / Lapsed |