Patent US4283383A successfully refreshed from Google Patents.
US4283383A
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Analysis of biological fluids
Assignee: Bayer Corp
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Filing: 1978-09-27
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Granted: 1981-08-11
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Expires: 1998-09-27
Abstract
Abstract Biological fluids, such as serum, are analyzed for the presence, nature and/or amount of antibodies, antigens and antibody:antigen complexes therein using as a reagent, insolubilized rheumatoid factor or insolubilized Clq. These reagents are themselves novel. They bind to antibody:antigen complexes, but not to free antibodies or antigens, and complexes can thus be removed from mixtures thereof with other materials, such as antibodies and antigens.
Inventors
- 👤 Pierre L. Masson
- 👤 Joseph F. Heremans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:02
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; G01N33/564; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/816; Y10S436/821
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
24 words
A reagent useful in biological analysis which comprises RF or Clq covalently bonded to a solid phase substrate which is insoluble in aqueous fluids.
Claim 2
Dependent Claim
24 words
A reagent useful in biological analysis which comprises RF or Clq covalently bonded to a solid phase substrate which is insoluble in aqueous fluids.
Claim 3
Independent Claim
110 words
A method for quantitatively analyzing a fluid sample for an antibody -antigen complex therein, comprising: (a) forming a mixture of the sample with a substance selected from the group consisting of RF and Clq, said substance being absorbed onto a solid phase substrate which is insoluble in aqueous fluids; (b) allowing the substance on the substrate selectively to react with antibody -antigen complex present in the mixture thereby selectively to bind said complex to said adsorbed substance to form a second complex; (c) assaying the second complex to determine the amount of antibody -antigen complex contained therein and thereby determining the amount of antibody -antigen complex present in the sample.
Claim 4
Independent Claim
110 words
A method for quantitatively analyzing a fluid sample for an antibody -antigen complex therein, comprising: (a) forming a mixture of the sample with a substance selected from the group consisting of RF and Clq, said substance being absorbed onto a solid phase substrate which is insoluble in aqueous fluids; (b) allowing the substance on the substrate selectively to react with antibody -antigen complex present in the mixture thereby selectively to bind said complex to said adsorbed substance to form a second complex; (c) assaying the second complex to determine the amount of antibody -antigen complex contained therein and thereby determining the amount of antibody -antigen complex present in the sample.
Claim 5
Dependent Claim
15 words
A method according to claim 2 wherein the solid phase is a naturally occurring material.
Claim 6
Dependent Claim
14 words
A method according to claim 3 wherein the solid phase substrate is aggregated immunoglobulins.
Claim 7
Dependent Claim
15 words
A method according to claim 2 wherein the solid phase substrate is a synthetic material.
Claim 8
Dependent Claim
14 words
A method according to claim 5 wherein the synthetic solid phase substrate is latex.
Claim 9
Dependent Claim
13 words
A method according to claim 2 wherein the substrate is in particulate form.
Claim 10
Dependent Claim
17 words
A method according to claim 2 wherein the substrate is the inner surface of a hollow tube.
Claim 11
Dependent Claim
22 words
A method according to claim 8 wherein the substrate reacts with Ab:Ag complex by passing the fluid sample through the hollow tube.
Claim 12
Independent Claim
162 words
A method of competitive binding assay by which the presence, in a sample, of a particular antibody, antigen or antibody -antigen complex may be detected, and the amount thereof determined, which comprises the steps of: (a) adding to the sample to form a mixture a quantity of the specific antigen or antibody for the antibody or antigen, respectively, to be determined, or a quantity of the antibody -antigen complex to be determined, which said quantity carries an identifying label; (b) contacting the mixture with a reagent substance selected from the group consisting of RF and Clq, said substance being adsorbed onto a solid phase substrate which is insoluble in aqueous fluids; (c) separating the reagent substance from the mixture; and (d) measuring by the identifying label the amount of labelled antibody, antigen, or antibody -antigen complex remaining in the mixture or separated with the reagent, thereby determining the presence and amount of antibody, antigen or antibody -antigen complex in the original sample.
Claim 13
Independent Claim
162 words
A method of competitive binding assay by which the presence, in a sample, of a particular antibody, antigen or antibody -antigen complex may be detected, and the amount thereof determined, which comprises the steps of: (a) adding to the sample to form a mixture a quantity of the specific antigen or antibody for the antibody or antigen, respectively, to be determined, or a quantity of the antibody -antigen complex to be determined, which said quantity carries an identifying label; (b) contacting the mixture with a reagent substance selected from the group consisting of RF and Clq, said substance being adsorbed onto a solid phase substrate which is insoluble in aqueous fluids; (c) separating the reagent substance from the mixture; and (d) measuring by the identifying label the amount of labelled antibody, antigen, or antibody -antigen complex remaining in the mixture or separated with the reagent, thereby determining the presence and amount of antibody, antigen or antibody -antigen complex in the original sample.
Claim 14
Independent Claim
121 words
A method for quantitatively analyzing an aqueous biological fluid sample for antibody or antigen therein, comprising: (a) adding to the sample the appropriate antibody or antigen, respectively, to form antibody -antigen complex; (b) forming a mixture of the sample with a substance selected from the group consisting of RF and Clq, the substance being adsorbed onto a solid phase substrate which is insoluble in aqueous fluids; (c) allowing the substance on the substrate selectively to react with antibody -antigen complex present in the mixture thereby selectively to bind the complex to the substance; and (d) assaying the substance to determine the amount of antibody -antigen complex bound thereto and thereby determining the amount of antibody or antigen present in the sample.
Claim 15
Independent Claim
121 words
A method for quantitatively analyzing an aqueous biological fluid sample for antibody or antigen therein, comprising: (a) adding to the sample the appropriate antibody or antigen, respectively, to form antibody -antigen complex; (b) forming a mixture of the sample with a substance selected from the group consisting of RF and Clq, the substance being adsorbed onto a solid phase substrate which is insoluble in aqueous fluids; (c) allowing the substance on the substrate selectively to react with antibody -antigen complex present in the mixture thereby selectively to bind the complex to the substance; and (d) assaying the substance to determine the amount of antibody -antigen complex bound thereto and thereby determining the amount of antibody or antigen present in the sample.
Claim 16
Dependent Claim
15 words
A method according to claim 11 wherein the solid phase is a naturally occurring material.
Claim 17
Dependent Claim
14 words
A method according to claim 12 wherein the solid phase substrate is aggregated immunoglobulins.
Claim 18
Dependent Claim
15 words
A method according to claim 11 wherein the solid phase substrate is a synthetic material.
Claim 19
Dependent Claim
14 words
A method according to claim 14 wherein the synthetic solid phase substrate is latex.
Claim 20
Dependent Claim
13 words
A method according to claim 11 wherein the substrate is in particulate form.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-08-10 | 1985-02-09 | 1985-08-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-08-10 | 1989-02-09 | 1989-08-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-08-10 | 1993-02-09 | 1993-08-11 | $7700.00 | Window Closed / Lapsed |