Cell culture medium supplement

Claim 2

A cell culture medium supplement comprising a solution of: approximately 0.336% by dry weight of potassium chloride; approximately 0.336% by dry weight of monobasic potassium phosphate; approximately 13.4% by dry weight of sodium chloride; approximately 1.93% by dry weight of dibasic sodium phosphate; and approximately 84.0% by dry weight of amino acids of such composition sufficient to support growth in the cells to be cultured, said amino acids comprising the following in approximate amounts: L-cystine-trace L-tyrosine 1.26% Lysine 6.25% Histidine 2.62% Arginine 3.45% Aspartic Acid 8.96% Threonine 4.30% Serine 4.75% Glutamic Acid 11.08% Proline 4.65% Glycine 5.98% Alanine 6.78% Valine 6.30% Methionine 1.93% Isoleucine 3.19% Leucine 7.26% Phenylalanine 4.35% Tryptophan 0.99% whereby the cell culture medium properties of blood serum are simulated.

Claim 3

The cell culture medium supplement of claim 1 wherein at least one said amino acid is radioactive.

Claim 4

The cell culture medium supplement of claim 1 further comprising a lipid whereby a lipidized serum is simulated.

Claim 5

A cell culture medium supplement comprising a solution of: approximately 0.336% by dry weight of potassium chloride; approximately 0.336% by dry weight of monobasic potassium phosphate; approximately 13.4% by dry weight of sodium chloride; approximately 1.93% by dry weight of dibasic sodium phosphate; and approximately 84.0% by dry weight of amino acids of such composition sufficient to suppport growth in the cells to be cultured, said amino acids comprising the following in approximate amounts: L-cystine-trace L-tyrosine 0.69% Lysine 3.79% Histidine 0.89% Arginine 7.76% Aspartic Acid 6.20% Threonine 1.82% Serine 3.73% Glutamic Acid 9.77% Proline 12.18% Glycine 19.37% Alanine 8.43% Valine 2.21% Methionine 1.01% Isoleucine 1.40% Leucine 2.90% Phenylalanine 1.88% Tryptophan 0.05% whereby the cell culture medium properties of blood serum are simulated.

Claim 6

A cell culture medium supplement comprising a solution of: approximately 0.336% by dry weight of potassium chloride; approximately 0.336% by dry weight of monobasic potassium phosphate; approximately 13.4% by dry weight of sodium chloride; approximately 1.93% by dry weight of dibasic sodium phosphate; and approximately 84.0% by dry weight of amino acids of such composition sufficient to suppport growth in the cells to be cultured, said amino acids comprising the following in approximate amounts: L-cystine-trace L-tyrosine 0.69% Lysine 3.79% Histidine 0.89% Arginine 7.76% Aspartic Acid 6.20% Threonine 1.82% Serine 3.73% Glutamic Acid 9.77% Proline 12.18% Glycine 19.37% Alanine 8.43% Valine 2.21% Methionine 1.01% Isoleucine 1.40% Leucine 2.90% Phenylalanine 1.88% Tryptophan 0.05% whereby the cell culture medium properties of blood serum are simulated.

Claim 7

A cell culture medium supplement comprising a solution of: approximately 0.336% by dry weight of potassium chloride; approximately 0.336% by dry weight of monobasic potassium phosphate; approximately 13.4% by dry weight of sodium chloride; approximately 1.93% by dry weight of dibasic sodium phosphate; and approximately 84.0% by dry weight of amino acids of such composition sufficient to support growth in the cells to be cultured, said amino acids comprising the following in approximate amounts: L-cystine-trace L-tyrosine 1.30% Lysine 6.49% Histidine 1.73% Arginine 2.79% Aspartic Acid 8.38% Threonine 3.94% Serine 4.27% Glutamic Acid 13.23% Proline 6.38% Glycine 8.38% Alanine 6.68% Valine 4.67% Methionine 1.96% Isoleucine 3.33% Leucine 6.97% Phenylalanine 3.22% Tryptophan 0.42% whereby the cell culture medium properties of blood serum are simulated.

Claim 8

A cell culture medium supplement comprising a solution of: approximately 0.336% by dry weight of potassium chloride; approximately 0.336% by dry weight of monobasic potassium phosphate; approximately 13.4% by dry weight of sodium chloride; approximately 1.93% by dry weight of dibasic sodium phosphate; and approximately 84.0% by dry weight of amino acids of such composition sufficient to support growth in the cells to be cultured, said amino acids comprising the following in approximate amounts: L-cystine-trace L-tyrosine 1.30% Lysine 6.49% Histidine 1.73% Arginine 2.79% Aspartic Acid 8.38% Threonine 3.94% Serine 4.27% Glutamic Acid 13.23% Proline 6.38% Glycine 8.38% Alanine 6.68% Valine 4.67% Methionine 1.96% Isoleucine 3.33% Leucine 6.97% Phenylalanine 3.22% Tryptophan 0.42% whereby the cell culture medium properties of blood serum are simulated.