US4281233A
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Hydrophilic macroporous three dimensional copolymers of hydroxyalkyl acrylates or methacrylates with crosslinking agents and the method of their manufacturing
Abstract The invention pertains to hydrophilic macroporous three dimensional copolymers of hydroxyalkyl methacrylates or hydroxyalkyl acrylates (C 1 -C 4 hydroxyalkyl) with crosslinking monomers which contain covalently attached saccharides via an O-glycosidic bond. The invention further pertains to the method of manufacturing of these copolymers by the direct reaction of the basic three dimensional copolymer containing hydroxyl groups with saccharides or their derivatives in an inert organic solvent under catalysis of HCl, BF 3 , or their mixtures at 20°-100° C. Saccharides and their derivatives are selected from the group comprising monosaccharides, oligosaccharides, deoxy sugars, amino saccharides, acylated saccharides, ether or halogen derivatives of monosaccharides and oligosaccharides, and the like. The invention also pertains to the application of these macroporous three dimensional copolymers with saccharides as sorbents and materials for the affinity chromatography for isolation of physiologically effective materials which contain free bonding sites for saccharides and are able to form specific complexes with saccharides.
An affinity chromatography process for the isolation of physiologically effective materials from active protein comprises: (a) applying a physiologic saline solution of said protein to a column of hydrophilic macroporous three dimensional copolymer of a hydroxyalkyl acrylate or a hydroxyalkyl methacrylate, said hydroxyalkyl containing 1 to 4 carbon atoms, and containing a crosslinking agent in the amount of 15 to 50 weight percent selected from the group consisting of ethylene diacrylate and ethylene dimethacrylate, wherein said copolymer contains, after glycosylation reaction at the surface, saccharides or their derivatives, selected from the group consisting of monosaccharides, oligosaccharides, deoxy sugars, amino sugars, acylated saccharides, either or halogen derivatives of monosaccharides and oligosaccharides, equilibrated with physiologic saline solution; (b) eluting said column with physiologic saline solution; and (c) recovering fractions active in haemaglutination.
Claim 2
Dependent Claim
130 words
An affinity chromatography process for the isolation of physiologically effective materials from active protein comprises: (a) applying a physiologic saline solution of said protein to a column of hydrophilic macroporous three dimensional copolymer of a hydroxyalkyl acrylate or a hydroxyalkyl methacrylate, said hydroxyalkyl containing 1 to 4 carbon atoms, and containing a crosslinking agent in the amount of 15 to 50 weight percent selected from the group consisting of ethylene diacrylate and ethylene dimethacrylate, wherein said copolymer contains, after glycosylation reaction at the surface, saccharides or their derivatives, selected from the group consisting of monosaccharides, oligosaccharides, deoxy sugars, amino sugars, acylated saccharides, either or halogen derivatives of monosaccharides and oligosaccharides, equilibrated with physiologic saline solution; (b) eluting said column with physiologic saline solution; and (c) recovering fractions active in haemaglutination.