Patent US4280992A successfully refreshed from Google Patents.
US4280992A
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Immunologically active substance-glass conjugates, process for producing the same and diagnostic reagents containing the same
Assignee: Sanyo Chemical Industries Ltd
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Filing: 1979-01-23
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Granted: 1981-07-28
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Expires: 1999-01-23
Abstract
Abstract An immunologically active substance-glass conjugate comprising an immunologically active substance physically or chemically bound to the surface of a frosted glass makes it possible to assay physiologically active substances in body fluids with high sensitivity.
Inventors
- 👤 Masakuzu Sugiura
- 👤 Junichiro Kikutake
- 👤 Masaru Yoshida
- 👤 Shigeharu Kondo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 16:08
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/551; G01N33/552; A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; C; C12; C12N; C12N11/00; C12N11/14; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
16 words
An immunologically active substance-glass conjugate which comprises an immunologically active substance bound to a frosted glass.
Claim 2
Dependent Claim
16 words
An immunologically active substance-glass conjugate which comprises an immunologically active substance bound to a frosted glass.
Claim 3
Dependent Claim
15 words
The conjugate of claim 1, which has a minimum diameter of at least 1 mm.
Claim 4
Dependent Claim
31 words
The conjugate of claim 1, wherein the immunologically active substance and the frosted glass are bound together with the aid of a silane coupling agent with or without a crosslinking agent.
Claim 5
Dependent Claim
35 words
The conjugate of claim 3, wherein the silane coupling agent has both a functional group reactive with the frosted glass and a functional group reactive with the immunologically active substance, the crosslinking agent, or both.
Claim 6
Dependent Claim
32 words
The conjugate of claim 3, wherein the silane coupling agent has both a functional group reactive with a silanol group and a functional group reactive with an amino, carboxyl or thiol group.
Claim 7
Dependent Claim
20 words
The conjugate of claim 5, wherein the functional group reactive with a silianol group is an alkoxysilyl or halosilyl group.
Claim 8
Dependent Claim
31 words
The conjugate of claim 5, wherein the functional group reactive with an amino, carboxyl or thiol group is a carboxyl, epoxy, haloalkyl, aldehyde, amino, thiol, isocyanate, carboxylate, imino or nitrile group.
Claim 9
Dependent Claim
22 words
The conjugate of claim 5, wherein the functional group reactive with the amino group is a carboxyl, epoxy, haloalkyl or aldehyde group.
Claim 10
Dependent Claim
20 words
The conjugate of claim 5, wherein the functional group reactive with a carboxyl group is an amino or epoxy group.
Claim 11
Dependent Claim
22 words
The conjugate of claim 5, wherein the functional group reactive with a thiol group is a thiol, epoxy, haloalkyl or aldehyde group.
Claim 12
Dependent Claim
26 words
The conjugate of claim 3, wherein the silane coupling agent is an alkoxysilyl group-containing silane coupling agent having an amino, thiol, epoxy, carboxyl or haloalkyl group.
Claim 13
Dependent Claim
24 words
The conjugate of claim 3, wherein the crosslinking agent is a compound which can crosslink the silane coupling agent and the immunologically active substance.
Claim 14
Dependent Claim
39 words
The conjugate of claim 3, wherein the crosslinking agent is a compound which can crosslink between an amino, carboxyl or thiol group of the silane coupling agent and an amino, carboxyl or thiol group of the immunologically active substance.
Claim 15
Dependent Claim
40 words
The conjugate of claim 3, wherein the crosslinking agent is a compound which can crosslink between amino groups, a compound which can crosslink between thiol groups or a compound which can crosslink between an amino group and a thiol group.
Claim 16
Dependent Claim
21 words
The conjugate of claim 14, wherein the compound which can crosslink between amino groups is an aliphatic dialdehyde or a dichlorotriazine.
Claim 17
Dependent Claim
18 words
The conjugate of claim 14, wherein the compound which can crosslink between thiol groups is a dimaleimide compound.
Claim 18
Dependent Claim
23 words
The conjugate of claim 14, wherein the compound which can crosslink between an amino group and a thiol group is a maleimidocarboxyl-N-hydroxysuccinimide ester.
Claim 19
Dependent Claim
13 words
The conjugate of claim 1, wherein the immunologically active substance is an antigen.
Claim 20
Dependent Claim
20 words
The conjugate of claim 18, wherein the antigen is a hormone, a protein, or an antigenic component of a pathogen.
Claim 21
Dependent Claim
19 words
The conjugate of claim 19, wherein the antigenic component of a pathogen is a pathogenic bacterium, virus or protozoon.
Claim 22
Dependent Claim
13 words
The conjugate of claim 1, wherein the immunologically active substance is an antibody.
Claim 23
Dependent Claim
19 words
The conjugate of claim 21, wherein the antibody is an antiserum obtained by immunizing a mammal with an antigen.
Claim 24
Dependent Claim
14 words
The conjugate of claim 1, wherein the frosted glass is a physically frosted glass.
Claim 25
Dependent Claim
18 words
The conjugate of claim 23, wherein the physically frosted glass is a glass abraded with an abrasive material.
Claim 26
Dependent Claim
14 words
The conjugate of claim 1, wherein the frosted glass is a chemically frosted glass.
Claim 27
Dependent Claim
28 words
The conjugate of claim 25, wherein the chemically frosted glass is a glass frosted by treatment with a frosting agent containing hydrofluoric acid, ammonium fluoride or an alkali.
Claim 28
Dependent Claim
20 words
The conjugate of claim 1, wherein the frosted glass has a shape of cylindrical, spherical, cubic or tubular glass body.
Claim 29
Dependent Claim
31 words
The conjugate of claim 1, wherein the frosted glass comprises a glass body having the maximum diameter of 1 to 20 mm and the minimum diameter of 1 mm or more.
Claim 30
Dependent Claim
17 words
The conjugate of claim 1, wherein the frosted glass has a frosting- degree of 1.5 to 10.0.
Claim 31
Dependent Claim
31 words
A process for producing an immunologically active substance-glass conjugate, which comprises reacting an immunologically active substance and a frosted glass with a silane coupling agent with or without a crosslinking agent.
Claim 32
Dependent Claim
31 words
A process for producing an immunologically active substance-glass conjugate, which comprises reacting an immunologically active substance and a frosted glass with a silane coupling agent with or without a crosslinking agent.
Claim 33
Dependent Claim
33 words
The process of claim 30, which comprises reacting a frosted glass with a silane coupling agent, and then reacting the resulting product with an immunologically active substance, with or without a crosslinking agent.
Claim 34
Dependent Claim
27 words
A process for immobilizing an immunologically active substance which comprises reacting an immunologically active substance with silane coupling agent-treated frosted glass, with or without a crosslinking agent.
Claim 35
Dependent Claim
27 words
A process for immobilizing an immunologically active substance which comprises reacting an immunologically active substance with silane coupling agent-treated frosted glass, with or without a crosslinking agent.
Claim 36
Dependent Claim
44 words
In a process for assaying a physiologically active substance in a body fluid by using a solid phase system, the improvement which comprises using as the solid phase system an immunologically active substance-glass conjugate comprising an immunologically active substance bound to a frosted glass.
Claim 37
Dependent Claim
44 words
In a process for assaying a physiologically active substance in a body fluid by using a solid phase system, the improvement which comprises using as the solid phase system an immunologically active substance-glass conjugate comprising an immunologically active substance bound to a frosted glass.
Claim 38
Dependent Claim
15 words
The process of claim 33, wherein assaying is carried out by enzyme-immunoassay or by radio-immunoassay.
Claim 39
Dependent Claim
13 words
The process of claim 33, wherein assaying is carried out by enzyme-immunoassay method.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-07-27 | 1985-01-26 | 1985-07-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-07-27 | 1989-01-26 | 1989-07-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-07-27 | 1993-01-26 | 1993-07-28 | $7700.00 | Window Closed / Lapsed |