US4278438A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for analysis of saccharides
Assignee: Individual
•
Filing: 1979-04-17
•
Granted: 1981-07-14
•
Expires: 1999-04-17
Abstract
Abstract An accurate new method and a new reagent for the chromatographic preparation and analysis of mixtures of both reducing and non-reducing saccharides is given, the mixture of saccharides being first separated, the oligosaccharides present in the obtained eluate fractions being hydrolyzed to monosaccharides and with the present monosaccharides being reacted with the new reagent to photometricably detectable compounds.
Inventors
- 👤 Willem Walraven
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 12:14
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/02; G01N33/14; G01N33/143; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
101 words
A process for separating and analyzing saccharides said process comprising, forming a second mixture comprising a mixture of saccharides possibly including oligosaccharides and at least one buffer and a reagent, flowing said second mixture through a separating column, and maintaining the temperature of said second mixture below 80° C. whereby premature reactions between said saccharides and said reagents are inhibited, hydrolyzing any oligosaccharides present in said second mixture to form monosaccharides, reacting with said reagent any monosaccharides originally present in said first mixture and also monosaccharides formed from said hydrolyzing step, and detecting the reaction products of said step of reacting.
Claim 2
Dependent Claim
101 words
A process for separating and analyzing saccharides said process comprising, forming a second mixture comprising a mixture of saccharides possibly including oligosaccharides and at least one buffer and a reagent, flowing said second mixture through a separating column, and maintaining the temperature of said second mixture below 80° C. whereby premature reactions between said saccharides and said reagents are inhibited, hydrolyzing any oligosaccharides present in said second mixture to form monosaccharides, reacting with said reagent any monosaccharides originally present in said first mixture and also monosaccharides formed from said hydrolyzing step, and detecting the reaction products of said step of reacting.
Claim 3
Dependent Claim
21 words
The process of claim 1 wherein said buffer is a borate having a pH in the range from 7.0 to 10.0.
Claim 4
Dependent Claim
19 words
The process of claim 1 wherein said reagent comprises an alkylene polyamine having at least two adjacent amino groups.
Claim 5
Dependent Claim
12 words
The process of claim 3 wherein said alkylene polyamine is ethylene diamine.
Claim 6
Dependent Claim
44 words
The process of claim 1 or 3 wherein said step of maintaining said second mixture below 80° C. further comprises maintaining a temperature of said second mixture above at least 25° C. during said step of flowing said second mixture through the separating column.
Claim 7
Dependent Claim
24 words
The process of claim 5 comprising maintaining the temperature of said second mixture between 120° C. and 180° C. during said step of reacting.
Claim 8
Dependent Claim
117 words
A process for separating and analyzing saccharides, said process comprising, forming a second mixture comprising a first mixture of saccharides possibly including oligosaccharides and at least one buffer and a reagent, said reagent comprising an alkylene polyamine having at least two adjacent amino groups, flowing said second mixture through a separating column, and maintaining the temperature of said second mixture below a selected maximum temperature whereby premature reactions between said saccharides and said reagents are inhibited, hydrolyzing any oligosaccharides present in said second mixture to form monosaccharides, reacting with said reagent any monosaccharides originally present in said first mixture and also monosaccharides formed from said hydrolyzing step, and detecting the reaction products of said step of reacting.
Claim 9
Dependent Claim
117 words
A process for separating and analyzing saccharides, said process comprising, forming a second mixture comprising a first mixture of saccharides possibly including oligosaccharides and at least one buffer and a reagent, said reagent comprising an alkylene polyamine having at least two adjacent amino groups, flowing said second mixture through a separating column, and maintaining the temperature of said second mixture below a selected maximum temperature whereby premature reactions between said saccharides and said reagents are inhibited, hydrolyzing any oligosaccharides present in said second mixture to form monosaccharides, reacting with said reagent any monosaccharides originally present in said first mixture and also monosaccharides formed from said hydrolyzing step, and detecting the reaction products of said step of reacting.
Claim 10
Dependent Claim
21 words
The process of claim 7 wherein said buffer is a borate having a pH in the range from 7.0 to 10.0.
Claim 11
Dependent Claim
12 words
The process of claim 7 wherein said alkylene polyamine is ethylene diamine.
Claim 12
Dependent Claim
50 words
The process of claim 7 or 9 wherein said step of maintaining said second mixture below a selected maximum temperature further comprises maintaining the temperature of said second mixture in the range from 25° C. to 80° C. during said step of flowing said second mixture through the separating column.
Claim 13
Dependent Claim
24 words
The process of claim 10 comprising maintaining the temperature of said second mixture between 120° C. and 180° C. during said step of reacting.
References Cited (Backward Citations)
US3375080A
Cited
US3630681A
Cited
US3694158A
Cited
US3694160A
Cited
US3785864A
Cited
US3806363A
Cited
US3817787A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-07-13 | 1985-01-12 | 1985-07-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-07-13 | 1989-01-12 | 1989-07-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-07-13 | 1993-01-12 | 1993-07-14 | $7700.00 | Window Closed / Lapsed |