US3785864A
Expired / Lapsed
US
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Process for the chromatographic separation of multi-component mixtures containing glucose
Assignee: Roche Diagnostics GmbH
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Filing: 1971-06-14
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Granted: 1974-01-15
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Expires: 1991-06-14
Abstract
Abstract Multi-component mixtures are chromatographically separated into their components on a large scale by using a separation column divided into two sections, passing the feed stream into the first section, removing the fractions containing impurities from the end of the first section, simultaneously passing an equivalent amount of elution agent into the second section, thereupon connecting the two column sections with each other until there appears at the end of the first section the fraction containing impurities from the next cycle of feed passed into the first section, and recovering the separated main components at the end of the second section.
Inventors
- 👤 K Lauer
- 👤 H Budka
- 👤 G Stoeck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:39
CPC Classifications
C; C13; C13K; C13K11/00; B; B01; B01D; B01D15/00; B01D15/08; B01D15/10; B01D15/18; B01D15/1864; B01D15/1871; C13B; C13B20/00; C13B20/12; C13K1/00; C13K1/06; C13K1/08
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
15 words
Process as claimed in claim 1 wherein said glucose syrup consists essentially of starch hydrolysates.
Claim 2
Dependent Claim
14 words
Process as claimed in claim 1 wherein said glucose syrup comprises non-epimerized starch syrup.
Claim 3
Dependent Claim
20 words
Process as claimed in claim 1 wherein said second period is about three times as long as said first period.
Claim 4
Dependent Claim
19 words
Process as claimed in claim 1 wherein said second column is about twice as long as said first column.
Claim 5
Dependent Claim
21 words
Process as claimed in claim 1 wherein the glucose syrup and the elution agent are supplied by means of dosing pumps.
Claim 6
Dependent Claim
62 words
Process as claimed in claim 1 wherein the constitution of the glucose product obtained at the end of said second column is analyzed by measuring the angle of rotation and the refractive index of the glucose product leaving the said second column and using such measurements in an analog calculator to direct the separation of said product into glucose and the contaminants.
Claim 7
Dependent Claim
35 words
Process as claimed in claim 1 wherein the removal of the fractions containing oligosaccharide impurities from the end of the first column is effected when the take-off from said first column contains substantially only oligosaccharides.
Claim 8
Dependent Claim
12 words
Process as claimed in claim 1 wherein the elution agent is water.
Claim 9
Dependent Claim
15 words
Process as claimed in claim 1 wherein said glucose syrup is an epimerized starch syrup.
Claim 10
Dependent Claim
73 words
Process as claimed in claim 10 wherein the ratio of the length of said first section of the total length of both column sections is substantially the same as the ratio of (a) the difference of the migration velocity of the glucose and the fructose to be recovered at the end of the second section to (b) the difference of the migration velocity of the glucose and the migration velocity of the impurities.
Claim 11
Dependent Claim
17 words
Process as claimed in claim 1 wherein said columns contain an ion exchanger loaded with calcium ions.
Claim 12
Dependent Claim
27 words
Process as claimed in claim 12 wherein at least one of the columns is loaded with polystyrene sulfonate resin cross-linked with divinylbenzene as a chromatographic separating medium.
Claim 13
Dependent Claim
40 words
Process as claimed in claim 1 wherein the separation of the contaminated fractions at the end of said first section is carried out by means of a valve controlled by a concentration analyzer and a control device operatively connected therewith.
Claim 14
Dependent Claim
22 words
Process as claimed in claim 14 wherein said concentration analyzer comprises a refractometer with a flow-through cuvette which provides a proportional voltage.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-01-14 | 1977-07-16 | 1978-01-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-01-14 | 1981-07-16 | 1982-01-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-01-14 | 1985-07-16 | 1986-01-15 | $7700.00 | Window Closed / Lapsed |