Patent US4273875A successfully refreshed from Google Patents.
US4273875A
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US
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Plasmid and process of isolating same
Assignee: Pharmacia and Upjohn Co
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Filing: 1979-03-05
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Granted: 1981-06-16
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Expires: 1999-03-05
Abstract
Abstract A novel chemical compound, essentially pure plasmid pUC6, which is obtainable from a biologically pure culture of the microorganism Streptomyces espinosus biotype 23724a, NRRL 11439. The pUC6 plasmid is useful as a cloning vehicle in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC6 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.
Inventors
- 👤 Jack J. Manis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:46
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/76; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/465; Y; Y10; Y10S; Y10S435/00; Y10S435/82; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
26 words
Essentially pure plasmid pUC6 which is characterized by a molecular weight of approximately 6.0 megadaltons, and a restriction endonuclease cleavage map as shown in the drawing.
Claim 2
Dependent Claim
26 words
Essentially pure plasmid pUC6 which is characterized by a molecular weight of approximately 6.0 megadaltons, and a restriction endonuclease cleavage map as shown in the drawing.
Claim 3
Dependent Claim
31 words
A biologically pure culture of Streptomyces espinosus biotype 23724a, having the deposit accession number NRRL 11439, and which also contains about 20 to about 40 copies of plasmid pUC6 per cell.
Claim 4
Dependent Claim
31 words
A biologically pure culture of Streptomyces espinosus biotype 23724a, having the deposit accession number NRRL 11439, and which also contains about 20 to about 40 copies of plasmid pUC6 per cell.
Claim 5
Dependent Claim
75 words
A process for isolating essentially pure pUC6 from Streptomyces espinosus biotype 23724a, NRRL 11439, which comprises: (a) growing S. espinosus biotype 23724a, NRRL 11439, on a suitable S. espinosus growth medium until sufficient mycelial growth is obtained; (b) fragmenting said mycelia; (c) incubating said fragmented mycelia in a suitable growth medium, as above; (d) harvesting the culture after a suitable time; (e) lysing the harvested mycelia; and (f) isolating essentially pure pUC6 from the lysate.
Claim 6
Dependent Claim
75 words
A process for isolating essentially pure pUC6 from Streptomyces espinosus biotype 23724a, NRRL 11439, which comprises: (a) growing S. espinosus biotype 23724a, NRRL 11439, on a suitable S. espinosus growth medium until sufficient mycelial growth is obtained; (b) fragmenting said mycelia; (c) incubating said fragmented mycelia in a suitable growth medium, as above; (d) harvesting the culture after a suitable time; (e) lysing the harvested mycelia; and (f) isolating essentially pure pUC6 from the lysate.
Claim 7
Dependent Claim
32 words
A process, according to claim 3, which comprises cultivating Streptomyces espinosus biotype 23724a, NRRL 11439, in a nutrient medium at a temperature of about 37° C. for about 36 to 48 hours.
Claim 8
Dependent Claim
20 words
A process, according to claim 3, wherein said fragmented mycelia is incubated in a growth medium containing sucrose and glycine.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-15 | 1984-12-15 | 1985-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-15 | 1988-12-15 | 1989-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-15 | 1992-12-15 | 1993-06-16 | $7700.00 | Window Closed / Lapsed |