Patent US4273557A successfully refreshed from Google Patents.
US4273557A
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US
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Limulus lysate procedure for determining endotoxins
Assignee: Lonza Walkersville Inc
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Filing: 1980-03-31
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Granted: 1981-06-16
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Expires: 2000-03-31
Abstract
Abstract A method and reagent for determining endotoxin in various fluids. The reagent comprises Limulus amebocyte lysate, a substantially linear polymeric water soluble interference-reducing agent and either calcium or magnesium cations.
Inventors
- 👤 David L. Juranas
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 04:12
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/579; Y; Y10; Y10S; Y10S436/00; Y10S436/825
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
167 words
In an in vitro test method for determining endotoxin under endotoxin determining conditions by Limulus amebocyte lysate in certain fluids selected from the group consisting of lipid emulsions for injection, parenterally administered sodium chloride solutions, lactated Ringer's solution, normal serum albumin, plasma protein fraction and anti-hemophilic factor USP which interferes with the reaction between any endotoxin present and said lysate thereby giving an improper determination, the improvement comprising conducting said method in the presence of (1) an interference reducing amount of a substantially linear polymeric water soluble interference reducing agent to reduce said interference, said agent having a molecular weight of at least 1000 selected from the group consisting of acrylic acid-acrylamide copolymer, acrylic acid--acrylonitrile copolymer, polyacrylic acid, alkali metal salts of polyacrylic acid, hydrolyzed polyacrylamide, alkali metal salts of hydrolyzed polyacrylamide, hydrolyzed polyacrylonitrile and alkali metal salts of polyacrylonitrile and (2) a sufficient amount of divalent cation selected from the group consisting of calcium and magnesium to overcome the chelating capacity of said interference reducing agent.
Claim 2
Dependent Claim
167 words
In an in vitro test method for determining endotoxin under endotoxin determining conditions by Limulus amebocyte lysate in certain fluids selected from the group consisting of lipid emulsions for injection, parenterally administered sodium chloride solutions, lactated Ringer's solution, normal serum albumin, plasma protein fraction and anti-hemophilic factor USP which interferes with the reaction between any endotoxin present and said lysate thereby giving an improper determination, the improvement comprising conducting said method in the presence of (1) an interference reducing amount of a substantially linear polymeric water soluble interference reducing agent to reduce said interference, said agent having a molecular weight of at least 1000 selected from the group consisting of acrylic acid-acrylamide copolymer, acrylic acid--acrylonitrile copolymer, polyacrylic acid, alkali metal salts of polyacrylic acid, hydrolyzed polyacrylamide, alkali metal salts of hydrolyzed polyacrylamide, hydrolyzed polyacrylonitrile and alkali metal salts of polyacrylonitrile and (2) a sufficient amount of divalent cation selected from the group consisting of calcium and magnesium to overcome the chelating capacity of said interference reducing agent.
Claim 3
Dependent Claim
47 words
A method according to claim 1, wherein the interference reducing agent is present in an amount of from about 0.05% to about 3% w/v and the divalent cation is present in an amount from about 0.05%-1.0 mole per mole of carboxyl groups of said interference reducing agent.
Claim 4
Dependent Claim
55 words
A method according to claim 2, wherein the interference reducing agent is selected from the group consisting of polyacrylic acid, polyacrylic acid sodium salt, a copolymer of acrylic acid and acrylamide, hydrolyzed polyacrylamide, the sodium salt of hydrolyzed polyacrylamide, hydrolyzed polyacrylonitrile and the sodium salt of hydrolyzed polyacrylonitrile and wherein the divalent cation is calcium.
Claim 5
Dependent Claim
20 words
A method according to claim 3, wherein the fluid is selected from the group consisting of lipid emulsions for injection.
Claim 6
Dependent Claim
21 words
A method according to claim 3, wherein the fluid is selected from the group consisting of parenterally administered sodium chloride solutions.
Claim 7
Dependent Claim
25 words
A method according to claim 3, wherein the fluid is selected from the group consisting of normal serum albumin, plasma protein fraction and anti-hemophilic factor.
Claim 8
Dependent Claim
97 words
An in vitro test reagent for determining endotoxin comprising Limulus amebocyte lysate having a sensitivity of at least about 0.25 mg/ml with US reference endotoxin EC-2, an interference reducing amount of a substantially linear polymeric water soluble interference reducing agent having a molecular weight of at least 1000 selected from the group consisting of acrylic acid-acrylamide copolymer, acrylic acid-acrylonitrile copolymer, polyacrylic acid, alkali metal and ammonium ammonium salts of hydrolyzed polyacrylamide, hydrolyzed polyacrylonitrile and alkali metal and ammonium salts of polyacrylonitrile and a sufficient amount of divalent cation selected from the group consisting of calcium and magnesium.
Claim 9
Dependent Claim
97 words
An in vitro test reagent for determining endotoxin comprising Limulus amebocyte lysate having a sensitivity of at least about 0.25 mg/ml with US reference endotoxin EC-2, an interference reducing amount of a substantially linear polymeric water soluble interference reducing agent having a molecular weight of at least 1000 selected from the group consisting of acrylic acid-acrylamide copolymer, acrylic acid-acrylonitrile copolymer, polyacrylic acid, alkali metal and ammonium ammonium salts of hydrolyzed polyacrylamide, hydrolyzed polyacrylonitrile and alkali metal and ammonium salts of polyacrylonitrile and a sufficient amount of divalent cation selected from the group consisting of calcium and magnesium.
Claim 10
Dependent Claim
24 words
A reagent according to claim 7, wherein the interference reducing agent is present in an amount of from about 0.05% to about 3% w/v.
Claim 11
Dependent Claim
44 words
A reagent according to claim 8, wherein the interference reducing agent is selected from the group consisting of polyacrylic acid, the sodium salt thereof, a copolymer of acrylic acid and acrylamide, hydrolyzed polyacrylamide, the sodium salt thereof, hydrolyzed polyacrylonitrile and the sodium salt thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-15 | 1984-12-15 | 1985-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-15 | 1988-12-15 | 1989-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-15 | 1992-12-15 | 1993-06-16 | $7700.00 | Window Closed / Lapsed |