Patent US4272433A successfully refreshed from Google Patents.
US4272433A
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Method for preparing antigen and antibody
Assignee: Otsuka Pharmaceutical Co Ltd
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Filing: 1979-09-20
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Granted: 1981-06-09
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Expires: 1999-09-20
Abstract
Abstract A method for preparing an antigen comprising a peptide-protein complex which comprises reacting a peptide represented by the general formula (I) ##STR1## wherein m is 0 or 1, as a hapten, with a protein as a carrier in the presence of a dialdehyde represented by the general formula (II) OHC--(CH.sub.2).sub.n --CHO (II) wherein n is an integer of 1 to 5 and a method for preparing an antibody specific to pancreatic glucagon comprising administering the above described antigen to a mammal and recovering the antibody produced.
Inventors
- 👤 Tomoyoshi Nishino
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 19:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; C; C07; C07K; C07K16/00; C07K16/18; C07K16/26; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/815; Y10S436/817; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
82 words
A peptide-protein complex antigen consisting essentially of a peptide of the formula (I) ##STR5## wherein m is 0 or 1, as a hapten, linked by a dialdehyde of the formula (II) OHC--(CH.sub.2).sub.n --CHO wherein n is an integer of 1 to 5, to a protein carrier selected from the group consisting of equine serum albumin, bovine serum albumin, rabbit serum albumin, human serum albumin, equine serum globulin, bovine serum globulin, rabbit serum globulin and human serum globulin conventionally used to prepare antigen.
Claim 2
Dependent Claim
82 words
A peptide-protein complex antigen consisting essentially of a peptide of the formula (I) ##STR5## wherein m is 0 or 1, as a hapten, linked by a dialdehyde of the formula (II) OHC--(CH.sub.2).sub.n --CHO wherein n is an integer of 1 to 5, to a protein carrier selected from the group consisting of equine serum albumin, bovine serum albumin, rabbit serum albumin, human serum albumin, equine serum globulin, bovine serum globulin, rabbit serum globulin and human serum globulin conventionally used to prepare antigen.
Claim 3
Dependent Claim
9 words
The antigen of claim 1, wherein m is 0.
Claim 4
Dependent Claim
9 words
The antigen of claim 1, wherein m is 1.
Claim 5
Dependent Claim
29 words
The antigen of claim 1, wherein there is present peptide of the formula (I), wherein m is 0 and also peptide of the formula (I) wherein m is 1.
Claim 6
Dependent Claim
14 words
The antigen of claim 1, wherein said dialdehyde is malonaldehyde, succinaldehyde, glutaraldehyde, or adipaldehyde.
Claim 7
Dependent Claim
22 words
The antigen of claim 1, wherein there is on the average 5 to 15 moles of peptide per mole of carrier protein.
Claim 8
Dependent Claim
20 words
The antigen of claim 6, containing on the average 9 to 12 moles of peptide per mole of carrier protein.
Claim 9
Dependent Claim
62 words
The antigen of claim 1, wherein the proportion by weight of said protein as a carrier to said peptide represented by the general formula (I) ranges from about 2:1 to about 6:1 and wherein the molar proportion of said dialdehyde represented by the general formula (II) to said peptide represented by the general formula (I) ranges from about 5:1 to about 10:1.
Claim 10
Dependent Claim
12 words
The antigen of claim 1, wherein the carrier is bovine serum albumin.
Claim 11
Dependent Claim
9 words
The antigen of claim 1, wherein n is 3.
Claim 12
Dependent Claim
12 words
The antigen of claim 10, wherein the carrier is bovine serum albumin.
Claim 13
Dependent Claim
9 words
The antigen of claim 10, wherein m is 0.
Claim 14
Dependent Claim
9 words
The antigen of claim 10, wherein m is 1.
References Cited (Backward Citations)
US3794630A
Cited
US4075194A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-08 | 1984-12-08 | 1985-06-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-08 | 1988-12-08 | 1989-06-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-08 | 1992-12-08 | 1993-06-09 | $7700.00 | Window Closed / Lapsed |