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US4271269A
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Regeneration of an enzyme immobilizate
Assignee: Dynamit Nobel AG
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Filing: 1979-05-15
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Granted: 1981-06-02
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Expires: 1999-05-15
Abstract
Abstract An enzyme immobilizate comprising an amorphous siliceous support to which an enzyme is bonded by adsorption via an amino alkoxy silane is regenerated after the enzyme has become inactive by contacting the immobilizate containing the inactive enzyme with a solution of inorganic ammonium sulfate or a solution of primary, secondary, tertiary or quaternary C 1 to C 6 alkyl or hydroxy alkyl ammonium sulfate to desorb the enzyme and then contacting the support with a fresh enzyme solution to adsorb active enzyme to the support.
Inventors
- 👤 Giselher Franzmann
- 👤 Hans-Leo Hulsmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 19:01
CPC Classifications
C; C12; C12P; C12P1/00; C12P13/00; C12P13/04; C12P13/22
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
114 words
A method for regeneration of an enzyme immobilizate comprising an amorphous silicecous support to which there is bonded by adsorption via an amino alkoxy silane containing an amino alkyl or amino aryl group an inactivated enzyme which comprises desorbing said inactivated enzyme from said immobilizate by contacting said immobilizate with a solution of inorganic ammonium sulfate or a primary, secondary, tertiary or quaternary C 1 to C 6 alkyl or hydroxy alkyl ammonium sulfate having a concentration of 10 to 40 weight percent and a pH in the range of 5 to 11 and thereafter reactivating the so-treated immobilizate by conctacting the same with fresh enzyme solution to adsorb fresh enzyme to said support.
Claim 2
Independent Claim
114 words
A method for regeneration of an enzyme immobilizate comprising an amorphous silicecous support to which there is bonded by adsorption via an amino alkoxy silane containing an amino alkyl or amino aryl group an inactivated enzyme which comprises desorbing said inactivated enzyme from said immobilizate by contacting said immobilizate with a solution of inorganic ammonium sulfate or a primary, secondary, tertiary or quaternary C 1 to C 6 alkyl or hydroxy alkyl ammonium sulfate having a concentration of 10 to 40 weight percent and a pH in the range of 5 to 11 and thereafter reactivating the so-treated immobilizate by conctacting the same with fresh enzyme solution to adsorb fresh enzyme to said support.
Claim 3
Dependent Claim
20 words
A method according to claim 1 wherein the salt solution has a pH in the range of 7 to 9.
Claim 4
Dependent Claim
20 words
A method according to claim 1 wherein the concentration of said ammonium sulfate solution is 20 to 30 weight percent.
Claim 5
Dependent Claim
15 words
A method according to claim 1 wherein the enzyme of the immobilizate is an acylase.
Claim 6
Dependent Claim
26 words
A method according to claim 1 wherein said solution is a solution of said primary, secondary, tertiary, or quaternary C 1 -C 6 alkyl ammonium sulfate.
Claim 7
Dependent Claim
26 words
A method according to claim 1, wherein said inactivated enzyme is removed from said immobilizate by contacting said immobilizate with said solution of inorganic ammonium sulfate.
References Cited (Backward Citations)
US3519538A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-01 | 1984-12-01 | 1985-06-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-01 | 1988-12-01 | 1989-06-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-01 | 1992-12-01 | 1993-06-02 | $7700.00 | Window Closed / Lapsed |