Patent US1097632A successfully refreshed from Google Patents.
Patent US4266031A successfully refreshed from Google Patents.
US4266031A
Expired / Lapsed
US
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Protease product of reduced allergenicity
Assignee: Novo Nordisk AS
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Filing: 1979-07-03
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Granted: 1981-05-05
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Expires: 1999-07-03
Abstract
Abstract A novel protease product suitable for admixture to washing compositions and exhibiting substantially attenuated allergenic properties is prepared by cultivating strains of Bacillus licheniformis which have been mutated to block their synthesis of protease other than Subtilopeptidase A (subtilisin). The commercial Bacillus licheniformis derived protease products are mixtures of subtilisin and a non-serine protease of lower stability and greater allergenicity than subtilisin.
Inventors
- 👤 Peter Tang
- 👤 Grethe C. Nielsen
- 👤 Keith Gibson
- 👤 Knud Aunstrup
- 👤 Hans Schiff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:01
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C12N9/96; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/836
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
64 words
A protease preparation having therein a B. licheniformis derived protease concentrate, wherein said protease concentrate: is stabilized by the presence therein of at least 0.5 percent by weight thereof of non-proteolytic peptides from the culture broth wherein the protease was generated; is essentially free of non-serine protease; and, exhibits substantially attenuated allergenic properties as compared to a non-serine protease containing B. licheniformis protease concentrate.
Claim 2
Dependent Claim
64 words
A protease preparation having therein a B. licheniformis derived protease concentrate, wherein said protease concentrate: is stabilized by the presence therein of at least 0.5 percent by weight thereof of non-proteolytic peptides from the culture broth wherein the protease was generated; is essentially free of non-serine protease; and, exhibits substantially attenuated allergenic properties as compared to a non-serine protease containing B. licheniformis protease concentrate.
Claim 3
Dependent Claim
29 words
The protease preparation of claim 1, in which at least 99 percent of the proteolytic activity of the protease concentrate incorporated therein is derived from the subtilisin isoenzyme system.
Claim 4
Dependent Claim
23 words
The protease preparation of claim 1, in which the protease concentrate contains therein 2-15 percent by weight of nonproteolytic culture broth derived peptides.
Claim 5
Dependent Claim
28 words
The protease preparation of claim 1, in which the proteolytic activity of the protease concentrate is in the range of from 2 to 20 Anson units per g.
Claim 6
Dependent Claim
92 words
A process for preparing a protease concentrate suitable for incorporation into the protease preparations adapted for admixture into washing compositions, said protease concentrate being essentially free of non-serine protease and exhibiting substantially attenuated allergenic properties as compared with a non-serine protease containing protease concentrate, characterized in that a strain of B. licheniformis, mutated to essentially block its capability for synthesizing other proteases than subtilisin, is cultivated in a nutrient medium containing assimilable sources of carbon, nitrogen and phosphorus, followed by recovery of the protease concentrate comprising subtilisin and culture broth derived peptides.
Claim 7
Dependent Claim
92 words
A process for preparing a protease concentrate suitable for incorporation into the protease preparations adapted for admixture into washing compositions, said protease concentrate being essentially free of non-serine protease and exhibiting substantially attenuated allergenic properties as compared with a non-serine protease containing protease concentrate, characterized in that a strain of B. licheniformis, mutated to essentially block its capability for synthesizing other proteases than subtilisin, is cultivated in a nutrient medium containing assimilable sources of carbon, nitrogen and phosphorus, followed by recovery of the protease concentrate comprising subtilisin and culture broth derived peptides.
Claim 8
Dependent Claim
26 words
The process of claim 5, in which the mutated B. licheniformis strain is selected from strains having been assigned deposit numbers NRRL B-11301, B-11302, and B-11303.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-05-04 | 1984-11-03 | 1985-05-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-05-04 | 1988-11-03 | 1989-05-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-05-04 | 1992-11-03 | 1993-05-05 | $7700.00 | Window Closed / Lapsed |