Macroporous polymeric carrier for covalently binding proteins, its preparation and its use for fixing active proteins

Claim 1

A macroporous crosslinked styrene resin, used as a carrier in which a biologically active protein is covalently bound via isocyanate, thiocyanate or aldehyde groups, wherein the sulfonic acid chloride groups of a sulfochlorinated macroporous crosslinked styrene resin, containing 1-4.5 milliequivalents/g of sulfonic acid chloride groups and consisting of 50-97% by weight of styrene and 3-50% by weight of divinylbenzene, with or without 1-25% by weight of ethylvinylbenzene and with or without 1-10% by weight, based on the total weight, of a further comonomer are reacted with an α,ω-diamino compound of the formula H.sub.2 N--(CH.sub.2).sub.n --NH.sub.2 where n is a number from 2 to 12, with hydrazine or with an α,ω-diamino-diether of the formula H.sub.2 N--(CH.sub.2).sub.3 --O--X--O--(CH.sub.2).sub.3 --NH.sub.2 where X is --(CH 2 ) n --, n being a number from 2 to 6, or where X is a 2,2-dimethyl-1,3-propylene or 3-methyl-1,5-pentylene radical, and any sulfonic acid chloride groups still remaining are hydrolyzed to the free sulfonic acid or saponified to its sodium salt or reacted with a primary or secondary amine to give sulfonamide groups, after which the terminal amino groups from the first process step are reacted with phosgene, thiophosgene, an aliphatic, aromatic or cycloaliphatic diisocyanate or an aliphatic dialdehyde of the formula OCH--(CH.sub.2).sub.n --CHO where n is a number from 1 to 8, to prepare the binding groups.