Patent US3980029A successfully refreshed from Google Patents.
Patent US4265880A successfully refreshed from Google Patents.
US4265880A
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US
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Microbial insecticide
Assignee: Washington State University
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Filing: 1980-03-26
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Granted: 1981-05-05
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Expires: 2000-03-26
Abstract
Abstract An improved microbial insecticide composition, and methods for the production and utilization thereof, are disclosed. The disclosed composition comprises a microbial insect pathogen of viral, bacterial, or fungal origin which is susceptible to sunlight-induced inactivation embedded in a coacervate microbead which is comprised of a nucleic acid, typically RNA, and a proteinaceous material, whereby the microbead structure itself effectively shields the agent from sunlight-induced inactivation. The microbead is typically stabilized by chemical crosslinking.
Inventors
- 👤 Kemet D. Spence
- 👤 Robert E. Andrews
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 11:43
CPC Classifications
A; A01; A01N; A01N25/00; A01N25/26; A01N25/28; A01N63/00; A01N63/20; A01N63/22; A01N63/23; A01N63/27; A01N63/40
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
79 words
A method of making a microbial insecticide composition, comprising (a) preparing a mixture comprising (i) nucleic acid particles, (ii) proteinaceous material particles, (iii) microbial insect pathogens of viral, bacterial, or fungal origin, and (iv) an amount of water sufficient to wet substantially the entire mixture and give it a paste-like consistency; and (b) agitating the paste-like mixture in a manner adapted to break up the mixture into discrete microbeads, whereby the microbial insect pathogens are embedded in the microbeads.
Claim 2
Independent Claim
79 words
A method of making a microbial insecticide composition, comprising (a) preparing a mixture comprising (i) nucleic acid particles, (ii) proteinaceous material particles, (iii) microbial insect pathogens of viral, bacterial, or fungal origin, and (iv) an amount of water sufficient to wet substantially the entire mixture and give it a paste-like consistency; and (b) agitating the paste-like mixture in a manner adapted to break up the mixture into discrete microbeads, whereby the microbial insect pathogens are embedded in the microbeads.
Claim 3
Dependent Claim
19 words
A method as in claim 1, wherein the discrete microbeads are stabilized by treatment with a chemical crosslinking agent.
Claim 4
Dependent Claim
15 words
A method as in claim 2, wherein the crosslinking agent comprises tannic acid or glutaraldehyde.
Claim 5
Dependent Claim
23 words
A method as in claim 2, wherein the agitation of the paste-like mixture takes place in a solution containing the chemical crosslinking agent.
Claim 6
Dependent Claim
38 words
A method as in claim 1, wherein the paste-like mixture is prepared by mixing the nucleic acid particles and the proteinaceous particles, separately mixing the microbial insect pathogens and the water, and then mixing these two mixtures together.
Claim 7
Dependent Claim
35 words
A method as in claim 1, wherein the paste-like mixture is prepared by first mixing the nucleic acid particles, the proteinaceous particles, and the water, and then mixing the microbial insect pathogens into this mixture.
Claim 8
Independent Claim
28 words
A method as in claims 1 or 9, wherein the nucleic acid comprises ribonucleic acid and the proteinaceous material comprises protamine, hemoglobin, or a synthetic amino acid polymer.
Claim 9
Dependent Claim
14 words
A method as in claim 1, wherein the discrete microbeads are subsequently freeze dried.
Claim 10
Independent Claim
67 words
A method of making a microbial insecticide composition, comprising: (a) preparing an aqueous solution containing a nucleic acid; (b) preparing an aqueous solution containing a proteinaceous material; (c) preparing an aqueous suspension of strongly positively or negatively surface-charged microbial insect pathogens; and (d) mixing the aqueous solutions and suspension prepared in steps (a), (b), and (c) together, thereby spontaneously forming microbeads having the insect pathogens embedded therein.
Claim 11
Independent Claim
67 words
A method of making a microbial insecticide composition, comprising: (a) preparing an aqueous solution containing a nucleic acid; (b) preparing an aqueous solution containing a proteinaceous material; (c) preparing an aqueous suspension of strongly positively or negatively surface-charged microbial insect pathogens; and (d) mixing the aqueous solutions and suspension prepared in steps (a), (b), and (c) together, thereby spontaneously forming microbeads having the insect pathogens embedded therein.
Claim 12
Dependent Claim
36 words
A method as in claim 9, wherein the suspension prepared in step (c) is first mixed with the solution prepared in step (a), and then this mixture is mixed with the solution prepared in step (b).
Claim 13
Dependent Claim
36 words
A method as in claim 9, wherein the suspension prepared in step (c) is first mixed with the solution prepared in step (b), and then this mixture is mixed with the solution prepared in step (a).
Claim 14
Dependent Claim
18 words
A method as in claim 9, wherein the microbeads are stabilized by treatment with a chemical crosslinking agent.
Claim 15
Independent Claim
43 words
A method as in claims 1 or 9, wherein prior to being embedded in the microbeads, the surface charge of the pathogens is made strongly negative or strongly positive by the addition of a protein-modifying agent to an aqueous suspension of the pathogens.
Claim 16
Dependent Claim
20 words
A method as in claim 9, wherein prior to treatment with the protein-modifying agent, the pathogens are cleaned by washing.
Claim 17
Dependent Claim
12 words
The insecticide composition made by the process of claims 1 or 8.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-05-04 | 1984-11-03 | 1985-05-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-05-04 | 1988-11-03 | 1989-05-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-05-04 | 1992-11-03 | 1993-05-05 | $7700.00 | Window Closed / Lapsed |