Patent US4264739A successfully refreshed from Google Patents.
US4264739A
Expired / Lapsed
US
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Sparger for cell culture system
Assignee: Merck and Co Inc
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Filing: 1979-01-05
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Granted: 1981-04-28
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Expires: 1999-01-05
Abstract
Abstract A sparger for mass cell culture systems is formed from an elongated tube having a plurality of ports.
Inventors
- 👤 Roy Grabner
- 👤 Edgar Scattergood
- 👤 Miguel O. Villarejos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 18:45
CPC Classifications
C; C12; C12M; C12M29/00; C12M29/06; Y; Y10; Y10S; Y10S435/00; Y10S435/818
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
149 words
In a gas sparger for a mass cell culture system, the improvement wherein the sparger comprises an elongated tube open at one end and sealed at the other end, the open end comprising a gas inlet means, the elongated tube having a plurality of indentations along its longitudinal axis, each indentation alternating with an unindented area of the elongated tube thereby providing the appearance of merlons, and two gas outlet channels originating beneath each unindented area and passing through the wall of the elongated tube wherein one of the two gas outlet channels passes angularly to the intended area on one side of the unindented area beneath which it orginates, and the other of the two gas outlet channels passes angularly to the indented area on the other side of the unindented area beneath which it originates thereby forming an angle between the two channels which is substantially V-shaped.
Claim 2
Dependent Claim
149 words
In a gas sparger for a mass cell culture system, the improvement wherein the sparger comprises an elongated tube open at one end and sealed at the other end, the open end comprising a gas inlet means, the elongated tube having a plurality of indentations along its longitudinal axis, each indentation alternating with an unindented area of the elongated tube thereby providing the appearance of merlons, and two gas outlet channels originating beneath each unindented area and passing through the wall of the elongated tube wherein one of the two gas outlet channels passes angularly to the intended area on one side of the unindented area beneath which it orginates, and the other of the two gas outlet channels passes angularly to the indented area on the other side of the unindented area beneath which it originates thereby forming an angle between the two channels which is substantially V-shaped.
Claim 3
Dependent Claim
22 words
A sparger according to claim 1 wherein the outlet ports have a diameter of from about 0.04 cm to about 0.14 cm.
Claim 4
Dependent Claim
17 words
A sparger according to claim 2 wherein the outlet ports have a diameter of about 0.09 cm.
Claim 5
Dependent Claim
37 words
A sparger according to claim 1 wherein the elongated tube containing the plurality of outlet ports has a threaded section adapted to be screwed into a threaded opening in the wall of the mass cell culture system.
Claim 6
Dependent Claim
31 words
A sparger according to claim 1 wherein some of the channels form an acute angle and some of the channels form an obtuse angle with the longitudinal axis of the tube.
Claim 7
Dependent Claim
25 words
A sparger according to claim 1 wherein the angles which each channel forms with the longitudinal axis of the tube are alternately acute and obtuse.
Claim 8
Dependent Claim
37 words
A method for aerating a mass cell culture system comprising passing the aerating gas to a sparger according to claim 1 and forcing the gas along the longitudinal axis of the tube and through the outlet channels.
Claim 9
Dependent Claim
22 words
A method according to claim 7 wherein the outlet ports have a diameter of from about 0.04 cm to about 0.14 cm.
Claim 10
Dependent Claim
17 words
A method according to claim 7 wherein the outlet ports have a diameter of about 0.09 cm.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-04-27 | 1984-10-27 | 1985-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-04-27 | 1988-10-27 | 1989-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-04-27 | 1992-10-27 | 1993-04-28 | $7700.00 | Window Closed / Lapsed |