Fluorescent labels

Claim 2

A composition comprising protein adsorbed or covalently bound to an aqueous-stabilized fluorescent label comprising a fluorescent rare earth chelate incorporated into a polymeric bead which is derived from a loaded latex wherein said rare earth chelate comprises up to about 7.5 percent by weight of said beads and said loaded latex has a polymeric discontinuous phase which consists essentially of polymer polymerized from one or more ethenic monomers and has an aqueous continuous phase and exhibits essentially no visible coagulation or settling out when 250 ml of the latex containing from about 10 to about 20 weight percent dispersed phase is stirred at 25° C. into an equal volume of acetone over a 1 minute period and subsequently allowed to stand at about 25° C. for 10 minutes.

Claim 3

The composition of claim 1 wherein said protein is selected from the group consisting of antibodies, antigens, and enzymes.

Claim 4

The composition of claim 1 wherein said protein is selected from the group consisting of antigens and antibodies.

Claim 5

The composition of claim 3 wherein the polymeric bead comprises a member selected from the group consisting of poly(n-butyl acrylate-co-styrene-co-2-acrylamido-2-methylpropanesulfonic acid, poly(n-butyl acrylate-co-2-acrylamido-2-methylpropanesulfonic acid, poly[styrene-co-(2-chloroethylsulfonylmethyl)styrene-co-2-acrylamido-2-methylpropanesulfonic acid], poly[styrene-co-acrylamido-co-(2-chloroethylsulfonylmethyl)styrene-co-2-acrylamido-2-methylpropanesulfonic acid], poly(n-butyl acrylate-co-styrene-co-m+p-chloromethylstyrene-co-2-acrylamido-2-methylpropanesulfonic acid, poly[n-butyl acrylate-co-styrene-co-m+p-chloromethylstyrene-co-2-(methacryloyloxy)ethyltrimethylammonium methosulfate], and poly[styrene-co-acrylamide-co-(2-chloroethylsulfonylmethyl)styrene].

Claim 6

The composition of claim 3 wherein the fluorescent rare earth chelate comprises a rare earth and a chelating agent selected from the group consisting of 1,3-diketones; phthalates, naphthalates; dipyridines and terpyridines; p-benzoylbenzoates, p-benzoylacetonates, and phenanthralines.

Claim 7

In a method for performing an immunoassay with labeled immunoreagents, the improvement wherein the labeled immunoreagent is an antigen or antibody absorbed or covalently bound to an aqueous-stabilized fluorescent label comprising fluorescent rare earth chelate incorporated into a polymeric bead which is derived from a loaded latex, wherein said rare earth chelate comprises up to about 7.5 percent by weight of said beads and said loaded latex has a polymeric discontinuous phase which consists essentially of polymer polymerized from one or more ethenic monomers and has an aqueous continuous phase and exhibits essentially no visible coagulation or settling out when 250 ml of the latex containing from about 10 to about 20 weight percent dispersed phase is stirred at 25° C. into an equal volume of acetone over a 1 minute period and subsequently allowed to stand at about 25° C. for 10 minutes.

Claim 8

In a method for performing an immunoassay with labeled immunoreagents, the improvement wherein the labeled immunoreagent is an antigen or antibody absorbed or covalently bound to an aqueous-stabilized fluorescent label comprising fluorescent rare earth chelate incorporated into a polymeric bead which is derived from a loaded latex, wherein said rare earth chelate comprises up to about 7.5 percent by weight of said beads and said loaded latex has a polymeric discontinuous phase which consists essentially of polymer polymerized from one or more ethenic monomers and has an aqueous continuous phase and exhibits essentially no visible coagulation or settling out when 250 ml of the latex containing from about 10 to about 20 weight percent dispersed phase is stirred at 25° C. into an equal volume of acetone over a 1 minute period and subsequently allowed to stand at about 25° C. for 10 minutes.