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US4259233A
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β-Galactosyl-umbelliferone-labeled protein and polypeptide conjugates
Assignee: Bayer Corp
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Filing: 1979-10-23
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Granted: 1981-03-31
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Expires: 1999-10-23
Abstract
Abstract A beta -galactosyl-umbelliferone-labeled conjugate of the formula: <IMAGE> +TR <IMAGE> wherein -NH)L is a protein or polypeptide, such as an immunoglobulin, bound through an amino group thereof, n is an integer from 2 through 10, m is an integer from 1 through 10, and p is on the average from 1 to the number of available amino groups in L. An intermediate in the synthesis of such labeled conjugate is also provided. The labeled conjugates are useful as reagents in specific binding assays (e.g., immunoassays) for determining the conjugated protein or polypeptide, or a specific binding analog or partner thereof, in liquid media.
Inventors
- 👤 Robert J. Carrico
- 👤 That T. Ngo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 09:47
CPC Classifications
C; C07; C07H; C07H21/00; C07H17/00; C07H17/04; C07H17/06; C07H17/065; C07H17/075; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/861; Y10S530/863
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
69 words
A β-galactosyl-umbelliferone-labeled conjugate of the formula: ##STR5## wherein --NH)L is a protein or polypeptide bound through an amino group thereof whereby said protein or polypeptide in the conjugate is capable of being bound by an antibody, n is an integer from 2 through 10, m is an integer from 1 through 10, and p is on the average from 1 to the number of available amino groups in L.
Claim 2
Dependent Claim
69 words
A β-galactosyl-umbelliferone-labeled conjugate of the formula: ##STR5## wherein --NH)L is a protein or polypeptide bound through an amino group thereof whereby said protein or polypeptide in the conjugate is capable of being bound by an antibody, n is an integer from 2 through 10, m is an integer from 1 through 10, and p is on the average from 1 to the number of available amino groups in L.
Claim 3
Dependent Claim
19 words
The conjugate of claim 1 wherein --NH)L is a protein or polypeptide of molecular weight between 130 and 10,000,000.
Claim 4
Dependent Claim
19 words
The conjugate of claim 1 wherein --NH)L is a protein or polypeptide of molecular weight between 1,000 and 1,000,000.
Claim 5
Dependent Claim
10 words
The conjugate of claim 1 wherein --NH)L is an immunoglobulin.
Claim 6
Dependent Claim
10 words
The conjugate of claim 4 wherein said immunoglobulin is IgG.
Claim 7
Dependent Claim
10 words
The conjugate of claim 4 wherein said immunoglobulin is IgM.
Claim 8
Dependent Claim
10 words
The conjugate of claim 4 wherein said immunoglobulin is IgA.
Claim 9
Dependent Claim
17 words
The conjugate of any of claims 1-7 wherein p is on the average from 1 to 100.
Claim 10
Dependent Claim
17 words
The conjugate of any of claims 1-7 wherein p is on the average from 1 to 20.
Claim 11
Dependent Claim
9 words
The conjugate of claim 9 wherein n=6 and m=4.
Claim 12
Dependent Claim
11 words
The conjugate of any of claims 1-7 wherein n=6 and m=4.
References Cited (Backward Citations)
US4182856A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-30 | 1984-09-29 | 1985-03-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-30 | 1988-09-29 | 1989-03-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-30 | 1992-09-29 | 1993-03-31 | $7700.00 | Window Closed / Lapsed |