Patent US4257774A successfully refreshed from Google Patents.
US4257774A
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Intercalation inhibition assay for compounds that interact with DNA or RNA
Assignee: BIOWHITTAKER, INC. A CORP. OF DELAWARE
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Filing: 1979-07-16
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Granted: 1981-03-24
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Expires: 1999-07-16
Abstract
Abstract A novel method for the detection, screening, the quantitation of compounds that interact with nucleic acids (DNA or RNA) is provided. The basis for this method is the inhibition of acridine orange binding to the nucleic acid. Measurement of binding inhibition utilizes the rapid, repeatable technique of fluorescence polarization.
Inventors
- 👤 Carol L. Richardson
- 👤 Gail E. Schulman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 22:58
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
146 words
A process for the quantitative determination of the extent of binding of a compound to a nucleic acid, which comprises the steps: (a) preparing a mixture containing said compound and an intercalator, said intercalator being selected from the group consisting of ethidium bromide, actinomycin D, proflavin and acridine orange, and determining the fluorescent polarization of said mixture; (b) adding the mixture of the compound and the intercalator to a nucleic acid under conditions suitable for the binding of both the compound and the intercalator to the nucleic acid, and determining the fluorescent polarization of the mixture containing the compound, the intercalator and the nucleic acid; and (c) determining the extent of binding of the compound to the nucleic acid from the difference in the fluorescent polarization of the mixture containing the compound and the intercalator and the mixture containing compound, the intercalator and the nucleic acid.
Claim 2
Dependent Claim
146 words
A process for the quantitative determination of the extent of binding of a compound to a nucleic acid, which comprises the steps: (a) preparing a mixture containing said compound and an intercalator, said intercalator being selected from the group consisting of ethidium bromide, actinomycin D, proflavin and acridine orange, and determining the fluorescent polarization of said mixture; (b) adding the mixture of the compound and the intercalator to a nucleic acid under conditions suitable for the binding of both the compound and the intercalator to the nucleic acid, and determining the fluorescent polarization of the mixture containing the compound, the intercalator and the nucleic acid; and (c) determining the extent of binding of the compound to the nucleic acid from the difference in the fluorescent polarization of the mixture containing the compound and the intercalator and the mixture containing compound, the intercalator and the nucleic acid.
Claim 3
Dependent Claim
17 words
A process according to claim 1 wherein the nucleic acid is a natural or synthetic nucleic acid.
Claim 4
Dependent Claim
14 words
A process according to claim 2 wherein the nucleic acid is DNA or RNA.
Claim 5
Dependent Claim
12 words
A process according to claim 3 wherein the intercalator is acridine orange.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-23 | 1984-09-22 | 1985-03-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-23 | 1988-09-22 | 1989-03-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-23 | 1992-09-22 | 1993-03-24 | $7700.00 | Window Closed / Lapsed |