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US4255454A
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Thermal destabilization of microbial rennet
Assignee: Novozymes AS
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Filing: 1978-12-28
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Granted: 1981-03-10
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Expires: 1998-12-28
Abstract
Abstract Microbial rennet having reduced thermal stability is produced by acylating the rennet with a monocarboxylic acid acyl radial of between 1 and 6 carbon atoms. Making cheese with the microbial rennet having reduced thermal stability enables the production of pasteurized whey having no residual rennet activity for use in human foods.
Inventors
- 👤 Sven Branner-Jorgensen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 15:00
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/96; A; A23; A23C; A23C19/00; A23C19/02; A23C19/032; A23C19/0326; C12N9/14; C12N9/48; C12N9/50; C12N9/58; C12N9/99; Y; Y10; Y10S; Y10S426/00; Y10S426/801
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
90 words
A method for producing microbial rennet having reduced thermal stability for use in making cheese comprising reacting microbial Mucor miehei rennet or Mucor pusillus rennet with a monocarboxylic acid acyl radial of between 1 and 6 carbon atoms in an aqueous medium at a pH between about 6 and about 11 and at a temperature between about 0° C. and about 40° C. to produce an acylated microbial rennet having reduced thermal stability of at least about 3° C. and having at least about 50% of the activity before acylating.
Claim 2
Independent Claim
90 words
A method for producing microbial rennet having reduced thermal stability for use in making cheese comprising reacting microbial Mucor miehei rennet or Mucor pusillus rennet with a monocarboxylic acid acyl radial of between 1 and 6 carbon atoms in an aqueous medium at a pH between about 6 and about 11 and at a temperature between about 0° C. and about 40° C. to produce an acylated microbial rennet having reduced thermal stability of at least about 3° C. and having at least about 50% of the activity before acylating.
Claim 3
Dependent Claim
17 words
Method according to claim 1 wherein the source of the monocarboxylic acid acyl radial is acetic anhydride.
Claim 4
Dependent Claim
17 words
Method according to claim 1 wherein the source of the monocarboxylic acid acyl radial is propionic anhydride.
Claim 5
Dependent Claim
28 words
Method according to claim 1 wherein the weight proportion of the monocarboxylic acid acyl radial to the total amount of microbial rennet protein is between 0.01 and 10.
Claim 6
Dependent Claim
28 words
Method according to claim 4 wherein the weight proportion of the monocarboxylic acid acyl radial to the total amount of microbial rennet protein is between 0.1 and 1.
Claim 7
Dependent Claim
34 words
A method for making cheese comprising coagulating milk with the rennet modified by acylation according to claim 1 to produce cheese and whey, pasteurizing the whey and using the whey to produce human food.
References Cited (Backward Citations)
US3549390A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-09 | 1984-09-08 | 1985-03-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-09 | 1988-09-08 | 1989-03-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-09 | 1992-09-08 | 1993-03-10 | $7700.00 | Window Closed / Lapsed |