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Patent US4254257A successfully refreshed from Google Patents.
US4254257A
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Amine salts of acidic microbial polysaccharides and their use in recovery of such polysaccharides
Assignee: Lubrizol Corp
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Filing: 1979-02-21
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Granted: 1981-03-03
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Expires: 1999-02-21
Abstract
Abstract Acidic polysaccharides of the type obtainable by microbial fermentation of an organic material such as a carbohydrate (e.g., xanthan gum) form novel amine salts with aliphatic or alicyclic polyamines having at least three amino nitrogen atoms and a molecular weight of at least 150. The amine salts may be used for isolation of the microbial polysaccharide from its fermentation broth by a process comprising the steps of acidifying, forming the amine salt by adding the amine or a salt thereof, and reducing the inorganic salt concentration as necessary (e.g., by dilution).
Inventors
- 👤 Calvin W. Schroeck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:00
CPC Classifications
C; C08; C08B; C08B37/00; C08B37/0006; C08B37/0024; C08B37/0033
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
39 words
A methanol-insoluble amine salt of an acidic polysaccharide produced by microbial fermentation, wherein the amine is at least one aliphatic or alicyclic polyamine having a molecular weight of at least 150 and containing at least three amino nitrogen atoms.
Claim 2
Dependent Claim
39 words
A methanol-insoluble amine salt of an acidic polysaccharide produced by microbial fermentation, wherein the amine is at least one aliphatic or alicyclic polyamine having a molecular weight of at least 150 and containing at least three amino nitrogen atoms.
Claim 3
Dependent Claim
54 words
An amine salt according to claim 1 wherein the amine is at least one alkylene polyamine of the formula ##STR2## wherein n is an integer which is at least 1, each R 1 is independently a divalent hydrocarbon radical having about 2-18 carbon atoms, and each R 2 is independently hydrogen or lower alkyl.
Claim 4
Dependent Claim
13 words
An amine salt according to claim 2 wherein the polysaccharide is xanthan gum.
Claim 5
Dependent Claim
32 words
An amine salt according to claim 3 wherein each R 1 is the same and is an alkylene radical having 2-6 carbon atoms and each R 2 is independently hydrogen or methyl.
Claim 6
Dependent Claim
13 words
An amine salt according to claim 4 wherein each R 2 is hydrogen.
Claim 7
Dependent Claim
16 words
An amine salt according to claim 5 wherein the amine is at least one ethylene polyamine.
Claim 8
Independent Claim
115 words
A method for recovering an acidic polysaccharide produced by microbial fermentation from an aqueous mixture comprising a fermentation broth which comprises the steps, performed in any order, of: (A) Adjusting the mixture to a final pH within the range of about 2.5-5.5; (B) Adding to the mixture at least one aliphatic or alicyclic polyamine having a molecular weight of at least 150 and containing at least three amino nitrogen atoms, or a salt of said amine, whereby an amine salt according to claims 1, 4, 5 or 6 is ultimately formed; and (C) Reducing the inorganic salt concentration of the mixture as necessary to effect precipitation of the amine salt of said acidic polysaccharide therefrom.
Claim 9
Dependent Claim
16 words
A method according to claim 7 wherein step A comprises adding an acid to the mixture.
Claim 10
Dependent Claim
22 words
A method according to claim 8 wherein the amount of acid added is such as to attain a final pH of 2.8-4.2.
Claim 11
Dependent Claim
14 words
A method according to claim 9 wherein the acid is a monobasic inorganic acid.
Claim 12
Dependent Claim
12 words
A method according to claim 10 wherein the acid is hydrochloric acid.
Claim 13
Dependent Claim
13 words
A method according to claim 11 wherein the acid is a monocarboxylic acid.
Claim 14
Dependent Claim
12 words
A method according to claim 12 wherein the acid is lactic acid.
Claim 15
Dependent Claim
17 words
A method according to claim 9 wherein step C is effected by diluting the mixture with water.
Claim 16
Dependent Claim
24 words
A method according to claim 14 wherein the amount of water used for dilution is about 0.1-5.0 parts by weight per part of broth.
Claim 17
Dependent Claim
18 words
A method according to claim 15 wherein the acid used in step A is a monobasic inorganic acid.
Claim 18
Dependent Claim
12 words
A method according to claim 16 wherein the acid is hydrochloric acid.
Claim 19
Dependent Claim
13 words
A method according to claim 15 wherein the acid is a monocarboxylic acid.
Claim 20
Dependent Claim
12 words
A method according to claim 16 wherein the acid is lactic acid.
Claim 21
Dependent Claim
18 words
A method according to claim 15 wherein the acid used in step A is a polybasic inorganic acid.
Claim 22
Dependent Claim
15 words
A method according to claim 9 wherein the final pH after step A is 3.7-3.8.
Claim 23
Independent Claim
102 words
A method for recovering xanthan gum from an aqueous mixture comprising a fermentation broth containing Xanthomonas campestris NRRL B-1459 which comprises the steps, performed in any order, of (A) adding hydrochloric acid to the mixture in an amount to attain a final pH of 3.7-3.8; (B) adding a commercially available amine mixture consisting predominantly of ethylene polyamines having a molecular weight of at least 150, or a salt of said amine mixture, whereby an amine salt of said xanthan gum is ultimately formed; (C) diluting with about 0.5-5.0 parts by weight of water per part of broth, and recovering the precipitated product.
Claim 24
Independent Claim
102 words
A method for recovering xanthan gum from an aqueous mixture comprising a fermentation broth containing Xanthomonas campestris NRRL B-1459 which comprises the steps, performed in any order, of (A) adding hydrochloric acid to the mixture in an amount to attain a final pH of 3.7-3.8; (B) adding a commercially available amine mixture consisting predominantly of ethylene polyamines having a molecular weight of at least 150, or a salt of said amine mixture, whereby an amine salt of said xanthan gum is ultimately formed; (C) diluting with about 0.5-5.0 parts by weight of water per part of broth, and recovering the precipitated product.
References Cited (Backward Citations)
US3232929A
Cited
US3119812A
Cited
US3281329A
Cited
US3244695A
Cited
US3280102A
Cited
US3349077A
Cited
US3316241A
Cited
US3328262A
Cited
US3422085A
Cited
US3598730A
Cited
US3632570A
Cited
US3729460A
Cited
US3711462A
Cited
US3773752A
Cited
US3928316A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-02 | 1984-09-01 | 1985-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-02 | 1988-09-01 | 1989-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-02 | 1992-09-01 | 1993-03-03 | $7700.00 | Window Closed / Lapsed |