US4254257A
Expired / Lapsed
US
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Amine salts of acidic microbial polysaccharides and their use in recovery of such polysaccharides
Assignee: Lubrizol Corp
•
Filing: 1979-02-21
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Granted: 1981-03-03
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Expires: 1999-02-21
Abstract
Abstract Acidic polysaccharides of the type obtainable by microbial fermentation of an organic material such as a carbohydrate (e.g., xanthan gum) form novel amine salts with aliphatic or alicyclic polyamines having at least three amino nitrogen atoms and a molecular weight of at least 150. The amine salts may be used for isolation of the microbial polysaccharide from its fermentation broth by a process comprising the steps of acidifying, forming the amine salt by adding the amine or a salt thereof, and reducing the inorganic salt concentration as necessary (e.g., by dilution).
Inventors
- 👤 Calvin W. Schroeck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 02:17
CPC Classifications
C; C08; C08B; C08B37/00; C08B37/0006; C08B37/0024; C08B37/0033
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
39 words
A methanol-insoluble amine salt of an acidic polysaccharide produced by microbial fermentation, wherein the amine is at least one aliphatic or alicyclic polyamine having a molecular weight of at least 150 and containing at least three amino nitrogen atoms.
Claim 2
Dependent Claim
39 words
A methanol-insoluble amine salt of an acidic polysaccharide produced by microbial fermentation, wherein the amine is at least one aliphatic or alicyclic polyamine having a molecular weight of at least 150 and containing at least three amino nitrogen atoms.
Claim 3
Dependent Claim
54 words
An amine salt according to claim 1 wherein the amine is at least one alkylene polyamine of the formula ##STR2## wherein n is an integer which is at least 1, each R 1 is independently a divalent hydrocarbon radical having about 2-18 carbon atoms, and each R 2 is independently hydrogen or lower alkyl.
Claim 4
Dependent Claim
13 words
An amine salt according to claim 2 wherein the polysaccharide is xanthan gum.
Claim 5
Dependent Claim
32 words
An amine salt according to claim 3 wherein each R 1 is the same and is an alkylene radical having 2-6 carbon atoms and each R 2 is independently hydrogen or methyl.
Claim 6
Dependent Claim
13 words
An amine salt according to claim 4 wherein each R 2 is hydrogen.
Claim 7
Dependent Claim
16 words
An amine salt according to claim 5 wherein the amine is at least one ethylene polyamine.
Claim 8
Independent Claim
115 words
A method for recovering an acidic polysaccharide produced by microbial fermentation from an aqueous mixture comprising a fermentation broth which comprises the steps, performed in any order, of: (A) Adjusting the mixture to a final pH within the range of about 2.5-5.5; (B) Adding to the mixture at least one aliphatic or alicyclic polyamine having a molecular weight of at least 150 and containing at least three amino nitrogen atoms, or a salt of said amine, whereby an amine salt according to claims 1, 4, 5 or 6 is ultimately formed; and (C) Reducing the inorganic salt concentration of the mixture as necessary to effect precipitation of the amine salt of said acidic polysaccharide therefrom.
Claim 9
Dependent Claim
16 words
A method according to claim 7 wherein step A comprises adding an acid to the mixture.
Claim 10
Dependent Claim
22 words
A method according to claim 8 wherein the amount of acid added is such as to attain a final pH of 2.8-4.2.
Claim 11
Dependent Claim
14 words
A method according to claim 9 wherein the acid is a monobasic inorganic acid.
Claim 12
Dependent Claim
12 words
A method according to claim 10 wherein the acid is hydrochloric acid.
Claim 13
Dependent Claim
13 words
A method according to claim 11 wherein the acid is a monocarboxylic acid.
Claim 14
Dependent Claim
12 words
A method according to claim 12 wherein the acid is lactic acid.
Claim 15
Dependent Claim
17 words
A method according to claim 9 wherein step C is effected by diluting the mixture with water.
Claim 16
Dependent Claim
24 words
A method according to claim 14 wherein the amount of water used for dilution is about 0.1-5.0 parts by weight per part of broth.
Claim 17
Dependent Claim
18 words
A method according to claim 15 wherein the acid used in step A is a monobasic inorganic acid.
Claim 18
Dependent Claim
12 words
A method according to claim 16 wherein the acid is hydrochloric acid.
Claim 19
Dependent Claim
13 words
A method according to claim 15 wherein the acid is a monocarboxylic acid.
Claim 20
Dependent Claim
12 words
A method according to claim 16 wherein the acid is lactic acid.
Claim 21
Dependent Claim
18 words
A method according to claim 15 wherein the acid used in step A is a polybasic inorganic acid.
Claim 22
Dependent Claim
15 words
A method according to claim 9 wherein the final pH after step A is 3.7-3.8.
Claim 23
Independent Claim
102 words
A method for recovering xanthan gum from an aqueous mixture comprising a fermentation broth containing Xanthomonas campestris NRRL B-1459 which comprises the steps, performed in any order, of (A) adding hydrochloric acid to the mixture in an amount to attain a final pH of 3.7-3.8; (B) adding a commercially available amine mixture consisting predominantly of ethylene polyamines having a molecular weight of at least 150, or a salt of said amine mixture, whereby an amine salt of said xanthan gum is ultimately formed; (C) diluting with about 0.5-5.0 parts by weight of water per part of broth, and recovering the precipitated product.
Claim 24
Independent Claim
102 words
A method for recovering xanthan gum from an aqueous mixture comprising a fermentation broth containing Xanthomonas campestris NRRL B-1459 which comprises the steps, performed in any order, of (A) adding hydrochloric acid to the mixture in an amount to attain a final pH of 3.7-3.8; (B) adding a commercially available amine mixture consisting predominantly of ethylene polyamines having a molecular weight of at least 150, or a salt of said amine mixture, whereby an amine salt of said xanthan gum is ultimately formed; (C) diluting with about 0.5-5.0 parts by weight of water per part of broth, and recovering the precipitated product.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-02 | 1984-09-01 | 1985-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-02 | 1988-09-01 | 1989-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-02 | 1992-09-01 | 1993-03-03 | $7700.00 | Window Closed / Lapsed |