US4252899A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for immobilizing glucose isomerase and a method of continuous isomerization of glucose
Assignee: Unilever Bestfoods North America
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Filing: 1979-01-15
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Granted: 1981-02-24
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Expires: 1999-01-15
Abstract
Abstract Glucose isomerase is immobilized in an active form by adsorbing the glucose isomerase onto a colloidal silica. The enzyme is contacted with the colloidal silica and the resulting composite solidified by freezing. Optionally, the composite may be gelatinized prior to freezing. The composite is then used for the isomerization of glucose to fructose.
Inventors
- 👤 Shigehiro Enokizono
- 👤 Soichiro Ushiro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 06:55
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12P; C12P19/00; C12P19/24; Y; Y10; Y10S; Y10S435/00; Y10S435/813
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
94 words
A continuous process for isomerizing glucose comprising contacting a glucose-containing solution having a pH of about 7 to about 9 with an immobilized enzyme composite comprising immobilized, active glucose isomerase on solid granules or flakes of colloidal silica having a particle size of 20 to 100 mesh and containing about 30 to 50 percent moisture, said immobilized enzyme composite being produced by adsorbing glucose isomerase enzyme onto colloidal silica by contacting enzyme with said colloidal silica in aqueous medium, solidifying the glucose isomerase adsorbed silica by freezing and then thawing the resultant frozen composition.
Claim 2
Dependent Claim
94 words
A continuous process for isomerizing glucose comprising contacting a glucose-containing solution having a pH of about 7 to about 9 with an immobilized enzyme composite comprising immobilized, active glucose isomerase on solid granules or flakes of colloidal silica having a particle size of 20 to 100 mesh and containing about 30 to 50 percent moisture, said immobilized enzyme composite being produced by adsorbing glucose isomerase enzyme onto colloidal silica by contacting enzyme with said colloidal silica in aqueous medium, solidifying the glucose isomerase adsorbed silica by freezing and then thawing the resultant frozen composition.
Claim 3
Dependent Claim
19 words
The process of claim 1 wherein the glucose isomerase adsorbed silica is treated with bifunctional reagent prior to freezing.
Claim 4
Dependent Claim
22 words
The process of claim 2 wherein said composite is comprised of colloidal silica of about 10 to about 30 mμ in diameter.
References Cited (Backward Citations)
US3669841A
Cited
US3796634A
Cited
US3933587A
Cited
US3935068A
Cited
US3956065A
Cited
US3930951A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-02-24 | 1984-08-25 | 1985-02-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-02-24 | 1988-08-25 | 1989-02-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-02-24 | 1992-08-25 | 1993-02-24 | $7700.00 | Window Closed / Lapsed |