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Patent US4252715A successfully refreshed from Google Patents.
US4252715A
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Chromogenic enzyme substrates
Assignee: Phadia AB
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Filing: 1979-07-02
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Granted: 1981-02-24
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Expires: 1999-07-02
Abstract
Abstract New chromogenic substrates suitable for serine proteases, specially suitable for diagnostic determination of factor Xa, which are represented by the following formula: R1-A1-A2-Gly-Arg-NH-R2 or its salts, where R1 is selected from the group of hydrogen, alkanoyl having from 1 to 12 carbon atoms, cyclohexylcarbonyl, benzoyl, benzoyl substituted with one or two halogen atoms, methylamine or phenyl groups, benzene sulphonyl and toluenesulphonyl; R2 is a chromophoric group; A1 is selected from the group of a single bond, and the amino acids selected from the group of Gly, Ala, Val, Leu, Ileu, Pro, Met, Phe and Tyr; and A2 is selected from the group of the amino acids Glu, Gln, Asp, and Asn.
Inventors
- 👤 Leif E. Aurell
- 👤 Karl G. Claeson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 04:04
CPC Classifications
C; C07; C07K; C07K5/00; C07K5/04; C07K5/08; C07K5/0819; C07K5/10; C07K5/1002; C07K5/1005; C07K5/101; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C12Q1/56; C12Q2337/00; C12Q2337/10; C12Q2337/12; C12Q2337/30; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/95; G01N2333/964; G01N2333/96425; G01N2333/96427; G01N2333/9643; G01N2333/96433; G01N2333/96441; G01N2333/96444; G01N2333/968; G01N2333/974; G01N2333/976; Y; Y10; Y10S; Y10S530/00; Y10S530/802; Y10S930/00; Y10S930/01; Y10S930/28
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
118 words
New chromogenic substrates suitable for serine proteases, specially suitable for diagnostic determination of factor Xa, which are represented by the following formula: R.sub.1 -A.sub.1 -A.sub.2 -Gly-Arg-NH-R.sub.2 or its salts, where R 1 is selected from the group of hydrogen, alkanoyl having from 1 to 12 carbon atoms, cyclohexylcarbonyl, benzoyl, benzoyl substituted with one or two halogen atoms, methylamine or phenyl groups, benzene sulphonyl and toluenesulphonyl; R 2 is a chromophoric group A 1 is selected from the group of a single bond, and the amino acids selected from the group of Gly, Ala, Val, Leu, Ileu, Pro, Met, Phe and Tyr; and A 2 is selected from the group of the amino acids Glu, Gln, Asp, and Asn.
Claim 2
Dependent Claim
118 words
New chromogenic substrates suitable for serine proteases, specially suitable for diagnostic determination of factor Xa, which are represented by the following formula: R.sub.1 -A.sub.1 -A.sub.2 -Gly-Arg-NH-R.sub.2 or its salts, where R 1 is selected from the group of hydrogen, alkanoyl having from 1 to 12 carbon atoms, cyclohexylcarbonyl, benzoyl, benzoyl substituted with one or two halogen atoms, methylamine or phenyl groups, benzene sulphonyl and toluenesulphonyl; R 2 is a chromophoric group A 1 is selected from the group of a single bond, and the amino acids selected from the group of Gly, Ala, Val, Leu, Ileu, Pro, Met, Phe and Tyr; and A 2 is selected from the group of the amino acids Glu, Gln, Asp, and Asn.
Claim 3
Dependent Claim
10 words
The substrate of claim 1 wherein R 1 is benzoyl.
Claim 4
Dependent Claim
10 words
The substrate of claim 1 wherein R 1 is H-Phe.
Claim 5
Dependent Claim
10 words
The substrate of claim 1 wherein A 1 is Ileu.
Claim 6
Dependent Claim
12 words
The substrate of claim 1 wherein A 1 is a single bond.
Claim 7
Dependent Claim
10 words
The substrate of claim 1 wherein A 1 is Leu.
Claim 8
Dependent Claim
10 words
The substrate of claim 1 wherein A 1 is Val.
Claim 9
Dependent Claim
20 words
The substrate of any one of claims 1, 2, 3, 4, 5, 6 or 7 wherein A 2 is Glu.
Claim 10
Dependent Claim
20 words
The substrate of any one of claims 1, 2, 3, 4, 5, 6, or 7 wherein A 2 is Asp.
Claim 11
Dependent Claim
20 words
The substrate of any one of claims 1, 2, 3, 4, 5, 6 or 7 wherein A 2 is Gln.
References Cited (Backward Citations)
US4028318A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-02-24 | 1984-08-25 | 1985-02-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-02-24 | 1988-08-25 | 1989-02-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-02-24 | 1992-08-25 | 1993-02-24 | $7700.00 | Window Closed / Lapsed |