Patent US4248971A successfully refreshed from Google Patents.
US4248971A
Expired / Lapsed
US
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Instant culture media and method of sterilizing same
Assignee: Individual
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Filing: 1978-06-08
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Granted: 1981-02-03
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Expires: 1998-06-08
Abstract
Abstract Disclosed are culture media comprising an aqueous solution of a gelling agent, a compound such as a bleach capable of sterilizing the gelling agent and its container. Also disclosed is a method for sterilizing a culture medium without autoclaving same by dissolving a gelling agent in boiling water; adding cool water to the aqueous gelling agent solution; dissolving a sterilizing agent in the cooled solution adding nutrients and then growing the culture.
Inventors
- 👤 Kamal A. Youssef
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:58
CPC Classifications
A; A61; A61L; A61L2/00; A61L2/16; A61L2/18; C; C12; C12N; C12N1/00; C12N5/00; A61L2103/00; A61L2103/05; Y; Y10; Y10S; Y10S435/00; Y10S435/80
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
69 words
A culture medium comprising: from about 0.1 to about 4.0 percent of a gelling agent selected from the group consisting of agar agar, pectin, silica gel and carageenan; from about 0.015 to about 0.03 weight percent of at least one halogen-liberating sterilizing compound compatible with said gelling agent and capable of sterilizing said gelling agent in aqueous medium; and from about 7 to about 50 weight percent of nutrients.
Claim 2
Dependent Claim
69 words
A culture medium comprising: from about 0.1 to about 4.0 percent of a gelling agent selected from the group consisting of agar agar, pectin, silica gel and carageenan; from about 0.015 to about 0.03 weight percent of at least one halogen-liberating sterilizing compound compatible with said gelling agent and capable of sterilizing said gelling agent in aqueous medium; and from about 7 to about 50 weight percent of nutrients.
Claim 3
Dependent Claim
21 words
The medium of claim 1, wherein said gelling agent, said sterilizing compound and said nutrients are provided in separate additive packages.
Claim 4
Dependent Claim
20 words
The medium of claim 1, wherein said sterilizing compound is sodium dichloro-s-triazinetrione dihydrate, sodium dichloro-isocyanurate dihydrate, trichloro-s-triazinetrione or trichloroisocyanuric acid.
Claim 5
Independent Claim
126 words
The method for forming and sterilizing a culture medium which comprises the steps of: dissolving from about 0.1 to about 4.0 percent by weight of a gelling agent in boiling water then adding cooler water at a temperature ranging from 15° to 25° C. to the resulting solution; mixing with the cooled solution from about 0.015 to 0.03 percent by weight of at least one halogen-liberating sterilizing compound compatible with said gelling agent and having a sterilizing effect thereon, said gelling agent being selected from the group of agar agar, pectin silica gel and carageenan; and adding from 7.0 to about 50 percent by weight of a concentrated solution of nutrients; pouring the culture to be grown into suitable sterile containers and cooling to room temperature.
Claim 6
Independent Claim
126 words
The method for forming and sterilizing a culture medium which comprises the steps of: dissolving from about 0.1 to about 4.0 percent by weight of a gelling agent in boiling water then adding cooler water at a temperature ranging from 15° to 25° C. to the resulting solution; mixing with the cooled solution from about 0.015 to 0.03 percent by weight of at least one halogen-liberating sterilizing compound compatible with said gelling agent and having a sterilizing effect thereon, said gelling agent being selected from the group of agar agar, pectin silica gel and carageenan; and adding from 7.0 to about 50 percent by weight of a concentrated solution of nutrients; pouring the culture to be grown into suitable sterile containers and cooling to room temperature.
Claim 7
Dependent Claim
24 words
The method of claim 4 wherein said solution after adding of said cooler water is brought to a temperature of about 45 degrees C.
Claim 8
Dependent Claim
20 words
The method of claim 4, wherein said sterilizing compound is sodium dichloro-s-triazinetrione dihydrate, sodium dichloro-isocyanurate dihydrate, trichloro-s-triazinetrione or trichloroisocyanuric acid.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-02-03 | 1984-08-04 | 1985-02-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-02-03 | 1988-08-04 | 1989-02-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-02-03 | 1992-08-04 | 1993-02-03 | $7700.00 | Window Closed / Lapsed |