Patent US4074249A successfully refreshed from Google Patents.
Patent US4246342A successfully refreshed from Google Patents.
US4246342A
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Process for the manufacture of pyruvate oxidase, and analytical method and kit for the use of the same
Assignee: Asahi Kasei Corp
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Filing: 1979-03-21
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Granted: 1981-01-20
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Expires: 1999-03-21
Abstract
Abstract Pyruvate oxidase can be produced by culturing Pediococcus sp. B-0667, Streptococcus sp. B-0668, Aerococcus viridans IFO-12219 or Aerococcus viridans IFO-12317. It is useful for analysis for pyruvic acid, because it catalyzes the reaction of pyruvic acid, phosphate and oxygen to form acetylphosphate, carbon dioxide and hydrogen peroxide. A kit containing the various reagents for such analysis is also provided by this invention.
Inventors
- 👤 Hideo Misaki
- 👤 Kazuo Matsuura
- 👤 Saburo Harada
- 👤 Satoshi Takenaka
- 👤 Yoshifumi Horiuchi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 08:59
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0008; C12Q; C12Q1/00; C12Q1/26; C12Y; C12Y102/00; C12Y102/03; C12Y102/03003; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/8215; Y10S435/822; Y10S435/885
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
41 words
A process for the manufacture of pyruvate oxidase, which comprises culturing a pyruvate oxidase-producing microorganism belonging to a genus selected from Pediococcus, Streptococcus and Aerococcus, in a nutrient culture medium, and separating the pyruvate oxidase thus produced from the culture medium.
Claim 2
Dependent Claim
41 words
A process for the manufacture of pyruvate oxidase, which comprises culturing a pyruvate oxidase-producing microorganism belonging to a genus selected from Pediococcus, Streptococcus and Aerococcus, in a nutrient culture medium, and separating the pyruvate oxidase thus produced from the culture medium.
Claim 3
Dependent Claim
31 words
A process as claimed in claim 1, in which said microorganism is selected from the group consisting of Pediococcus sp. B-0667, Streptococcus sp. B-0668, Aerococcus viridans IFO-12219 and Aerococcus viridans IFO-12317.
Claim 4
Independent Claim
65 words
A method for the analysis of pyruvic acid in a sample containing pyruvic acid or a pyruvate-liberating system, comprising contacting said sample with pyruvate oxidase and measuring a consumed component or a generated component, in which said reaction system contains at least pyruvate oxidase, FAD, thiamine pyrophosphate, phosphate and a salt which liberates ions selected from the group consisting of calcium cobalt, magnesium and manganese.
Claim 5
Independent Claim
65 words
A method for the analysis of pyruvic acid in a sample containing pyruvic acid or a pyruvate-liberating system, comprising contacting said sample with pyruvate oxidase and measuring a consumed component or a generated component, in which said reaction system contains at least pyruvate oxidase, FAD, thiamine pyrophosphate, phosphate and a salt which liberates ions selected from the group consisting of calcium cobalt, magnesium and manganese.
Claim 6
Dependent Claim
18 words
A method as claimed in claim 3, in which said system also contains an indicator for hydrogen peroxide.
Claim 7
Dependent Claim
28 words
A method as claimed in claim 4, in which said indicator for hydrogen peroxide is selected from the group consisting of peroxidase, 4-aminoanitipyrin, phenol, N,N-dimethylaniline and homovanillic acid.
Claim 8
Dependent Claim
20 words
A method as claimed in claim 3, in which a consumed component is measured and said consumed component is oxygen.
Claim 9
Dependent Claim
21 words
A method as claimed in claim 3, in which a generated component is measured and said generated component is hydrogen peroxide.
Claim 10
Dependent Claim
51 words
A kit for the analysis of pyruvic acid, comprising a plurality of separate compositions one of which contains at least pyruvate oxidase, FAD, thiamine pyrophosphate and phosphate and another of which contains at least a salt which liberates an ion selected from the group consisting of calcium, cobalt, magnesium and manganese.
Claim 11
Dependent Claim
51 words
A kit for the analysis of pyruvic acid, comprising a plurality of separate compositions one of which contains at least pyruvate oxidase, FAD, thiamine pyrophosphate and phosphate and another of which contains at least a salt which liberates an ion selected from the group consisting of calcium, cobalt, magnesium and manganese.
Claim 12
Dependent Claim
14 words
A kit as claimed in claim 8, containing also an indicator for hydrogen peroxide.
Claim 13
Dependent Claim
30 words
A kit as claimed in claim 9, in which said indicator for hydrogen peroxide is a member selected from the group consisting of peroxidase, 4-aminoanitipyrin, phenol, N,N-dimethylaniline and homovanillic acid.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-01-20 | 1984-07-21 | 1985-01-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-01-20 | 1988-07-21 | 1989-01-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-01-20 | 1992-07-21 | 1993-01-20 | $7700.00 | Window Closed / Lapsed |