Patent US3174890A successfully refreshed from Google Patents.
Patent US4246222A successfully refreshed from Google Patents.
US4246222A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Gel slab casting
Assignee: Bio Rad Laboratories Inc
•
Filing: 1979-10-22
•
Granted: 1981-01-20
•
Expires: 1999-10-22
Abstract
Abstract A method and apparatus for casting gel slabs useful in electrophoretic procedures is provided. Glass plates are horizontally positioned in parallel a capillary action-inducing distance apart to form an unsealed space. Gel-forming liquid, e.g. polyacrylamide is introduced between the plates and fills the space by capillary action. The surface tension of the gel-forming liquid is utilized to constrain the liquid between the plates at the unsealed or open edges. Once the desired sized space is filled the gel is formed by polymerization and/or cooling. Where an open faced gel slab is desired one of the plates is separated from the gel layer.
Inventors
- 👤 James F. Monthony
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 18:29
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; B; B29; B29C; B29C39/00; B29C39/02; B29C39/10; B29C39/22; B29C39/24; B29C39/26; B29C39/265; B29C70/00; B29C70/68; B29C70/78; B29K; B29K2033/00; B29K2105/00; B29K2105/0002; B29K2105/0058; B29K2105/0061; B29K2909/00; B29K2909/08
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
138 words
A method of casting a gel slab useful in electrophoretic procedures, comprising the steps of (a) forming a space having first and second opposing open edges between the facing surfaces of parallel horizontally positioned upper and lower plates separated a predetermined capillary action-inducing distance apart; (b) introducing a gel forming liquid into said space at one of said open edges whereby said liquid is an amount substantially equal in volume to the volume of said space along the entirety of said first opposing edge in a manner which produces a front of said liquid, said front being drawn into said space by capillary action thereby displacing and expelling air out said second opposing open edge and is contained therein by surface tension; and (c) forming from said liquid a gel adhered to at least one of said surfaces.
Claim 2
Independent Claim
138 words
A method of casting a gel slab useful in electrophoretic procedures, comprising the steps of (a) forming a space having first and second opposing open edges between the facing surfaces of parallel horizontally positioned upper and lower plates separated a predetermined capillary action-inducing distance apart; (b) introducing a gel forming liquid into said space at one of said open edges whereby said liquid is an amount substantially equal in volume to the volume of said space along the entirety of said first opposing edge in a manner which produces a front of said liquid, said front being drawn into said space by capillary action thereby displacing and expelling air out said second opposing open edge and is contained therein by surface tension; and (c) forming from said liquid a gel adhered to at least one of said surfaces.
Claim 3
Dependent Claim
30 words
The method of claim 1 wherein one of said surfaces is relatively non-adherent to said gel; and further comprising the step of (d) releasing said gel from said non-adherent surface.
Claim 4
Dependent Claim
30 words
A method according to claim 1 wherein said step of forming further comprises forming said space between one lower plate and at least first, second and third contiguous upper plates.
Claim 5
Dependent Claim
42 words
A method according to claim 3 wherein said first and said third upper plates, each contiguous to said second upper plate have relatively non-gel-adherent surfaces opposing said lower plate, and further comprising the step of removing said non-adherent surfaces from said gel.
Claim 6
Dependent Claim
32 words
A method according to claim 4 wherein said first upper plate is a sample well forming bar, and said gel-forming step further comprises forming a gel having at least one sample well.
Claim 7
Dependent Claim
17 words
A method according to claim 4 wherein said third upper plate is a wick area forming bar.
Claim 8
Independent Claim
101 words
A method of casting a gel slab useful in electrophoretic procedures, comprising the steps of (a) forming a space having first and second opposing open edges between the facing surfaces of parallel horizontally positioned upper and lower plates separated a predetermined capillary action-inducing distance of from 0.25-3.5 mm apart; (b) introducing an acrylamide monomer solution into said space at one of said open edges whereby said solution is drawn into and fills said space by capillary action and is contained therein by surface tension; and (c) forming from said solution a polyacrylamide gel adhered to at least one of said surfaces.
Claim 9
Independent Claim
101 words
A method of casting a gel slab useful in electrophoretic procedures, comprising the steps of (a) forming a space having first and second opposing open edges between the facing surfaces of parallel horizontally positioned upper and lower plates separated a predetermined capillary action-inducing distance of from 0.25-3.5 mm apart; (b) introducing an acrylamide monomer solution into said space at one of said open edges whereby said solution is drawn into and fills said space by capillary action and is contained therein by surface tension; and (c) forming from said solution a polyacrylamide gel adhered to at least one of said surfaces.
Claim 10
Independent Claim
155 words
A method of casting a plurality of gel salbs useful in electrophoretic procedures, comprising the steps of: (a) forming at least two spaces, each of said spaces having first and second opposing open edges between the facing surfaces of parallel horizontally positioned upper and lower plates separated a predetermined capelliary action-inducing distance apart, (b) introducing a gel forming liquid into each of said spaces at one of said open edges whereby said liquid is an amount substantially equal in volume to the volume of each said space along the entirety of each said first opposing edge in a manner which produces a front of said liquid, said front being drawn into each said space by capillary action thereby displacing and expelling air out of each said second opposing open edge and is contained therein by surface tension; and (c) forming from said liquid a gel adhered to at least of the surfaces of said spaces.
Claim 11
Independent Claim
155 words
A method of casting a plurality of gel salbs useful in electrophoretic procedures, comprising the steps of: (a) forming at least two spaces, each of said spaces having first and second opposing open edges between the facing surfaces of parallel horizontally positioned upper and lower plates separated a predetermined capelliary action-inducing distance apart, (b) introducing a gel forming liquid into each of said spaces at one of said open edges whereby said liquid is an amount substantially equal in volume to the volume of each said space along the entirety of each said first opposing edge in a manner which produces a front of said liquid, said front being drawn into each said space by capillary action thereby displacing and expelling air out of each said second opposing open edge and is contained therein by surface tension; and (c) forming from said liquid a gel adhered to at least of the surfaces of said spaces.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-01-20 | 1984-07-21 | 1985-01-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-01-20 | 1988-07-21 | 1989-01-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-01-20 | 1992-07-21 | 1993-01-20 | $7700.00 | Window Closed / Lapsed |